PMID- 34362270
OWN - NLM
STAT- MEDLINE
DCOM- 20220920
LR  - 20231031
IS  - 1708-539X (Electronic)
IS  - 1708-5381 (Linking)
VI  - 30
IP  - 5
DP  - 2022 Oct
TI  - The protective mechanism of folic acid on hyperhomocysteinemia-related arterial 
      injury in spontaneously hypertensive rats: Folic acid against arterial 
      inflammation.
PG  - 988-998
LID - 10.1177/17085381211036549 [doi]
AB  - BACKGROUND: Hypertension associated with hyperhomocysteinemia (HHcy) is 
      correlated with a high risk of vascular diseases. Studies found that folic acid 
      (FA) supplementation can reduce the risk of cardiovascular and cerebrovascular 
      events. The aim of the present study was to explore the potential mechanisms of 
      FA attenuating HHcy-related arterial injury in spontaneously hypertensive rats 
      (SHRs). METHODS: 24 SHRs were randomized into the control group, the HHcy group, 
      and the HHcy + FA group (8 per group). The SHRs in the HHcy group and the HHcy + 
      FA group were given DL-Hcy intraperitoneally to mimic hypertension associated 
      with HHcy. The SHRs in the HHcy + FA group were given FA by gavage to mimic an 
      FA-fortified diet. The histopathology and immunohistochemistry of rat aorta and 
      carotid artery were analyzed, and the relative expression levels of 
      immune/inflammation and oxidative stress molecules in arterial tissue were 
      detected by quantitative real-time polymerase chain reaction (qRT-PCR) and 
      Western blot. RESULTS: FA significantly reduced the expression levels of nuclear 
      factor-kappa-gene binding (NF-kappaB) p65/Rela and interleukin-6 (IL-6) in rat arterial 
      tissues, as well as the levels of plasma HHcy and serum malondialdehyde (MDA) in 
      hypertension associated with HHcy rats (p < 0.05). At the same time, FA 
      significantly increased the serum superoxide dismutase (SOD) level in 
      hypertension associated with HHcy rats, and even the SOD level of the HHcy + FA 
      group was higher than that of the control group (p < 0.05). However, HHcy induced 
      the opposite results of the above indicators in SHRs compared with the control 
      group (p < 0.05). CONCLUSIONS: The arterial protection mechanisms of FA are 
      related to reducing the concentration of HHcy to eliminate the tissue toxicity of 
      HHcy, inhibiting NF-kappaBp65/Rela/IL-6 pathway molecules to regulate inflammatory 
      response, and promoting the potential anti-oxidative stress pathway molecules to 
      reduce oxidative stress level.
FAU - Zhang, Lihua
AU  - Zhang L
AD  - Shandong Provincial Qianfoshan Hospital, 74738Shandong University, Jinan, 
      Shandong, China.
AD  - Department of Medicine, Jinan Maternity and Child Care Hospital Affiliated to 
      Shandong First Medical University, Jinan, China.
FAU - Li, Zhongliang
AU  - Li Z
AD  - Department of Women Healthcare, Jinan Maternity and Child Care Hospital 
      Affiliated to Shandong First Medical University, Jinan, China.
FAU - Xing, Changcheng
AU  - Xing C
AD  - 74738Shandong University of Traditional Chinese Medicine, Jinan, China.
FAU - Ma, Xiaoshan
AU  - Ma X
AD  - Department of Medicine, Jinan Maternity and Child Care Hospital Affiliated to 
      Shandong First Medical University, Jinan, China.
FAU - Xu, Rui
AU  - Xu R
AUID- ORCID: 0000-0002-6212-707X
AD  - Shandong Provincial Qianfoshan Hospital, 74738Shandong University, Jinan, 
      Shandong, China.
AD  - Department of Cardiology, 74738The First Affiliated Hospital of Shandong First 
      Medical University, Jinan, China.
LA  - eng
PT  - Journal Article
DEP - 20210806
PL  - England
TA  - Vascular
JT  - Vascular
JID - 101196722
RN  - 0 (Interleukin-6)
RN  - 0 (NF-kappa B)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - 935E97BOY8 (Folic Acid)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
SB  - IM
MH  - Animals
MH  - *Arteritis/complications
MH  - Folic Acid/pharmacology
MH  - *Hyperhomocysteinemia/complications/drug therapy
MH  - *Hypertension/complications/drug therapy
MH  - Interleukin-6
MH  - Malondialdehyde/metabolism
MH  - NF-kappa B
MH  - Rats
MH  - Rats, Inbred SHR
MH  - Superoxide Dismutase/metabolism
OTO - NOTNLM
OT  - Folic acid
OT  - NADPH oxidase
OT  - NF-kappaB
OT  - hypertension associated with hyperhomocysteinemia
OT  - immune/inflammation
OT  - oxidative stress
EDAT- 2021/08/08 06:00
MHDA- 2022/09/21 06:00
CRDT- 2021/08/07 05:22
PHST- 2021/08/08 06:00 [pubmed]
PHST- 2022/09/21 06:00 [medline]
PHST- 2021/08/07 05:22 [entrez]
AID - 10.1177/17085381211036549 [doi]
PST - ppublish
SO  - Vascular. 2022 Oct;30(5):988-998. doi: 10.1177/17085381211036549. Epub 2021 Aug 
      6.