PMID- 34364580
OWN - NLM
STAT- MEDLINE
DCOM- 20220105
LR  - 20220105
IS  - 1879-1344 (Electronic)
IS  - 0144-8617 (Linking)
VI  - 271
DP  - 2021 Nov 1
TI  - Cordyceps polysaccharide marker CCP modulates immune responses via highly 
      selective TLR4/MyD88/p38 axis.
PG  - 118443
LID - S0144-8617(21)00830-4 [pii]
LID - 10.1016/j.carbpol.2021.118443 [doi]
AB  - Cordyceps, one of the most expensive natural health supplements, is popularly 
      used to modulate immune function. However, little is known regarding the 
      underlying mechanism of its immunomodulatory activity. We newly reported a 
      Cordyceps quality marker CCP (Mw 433.778 kDa) which was characterized as a 1,4-alpha 
      glucan by chemical and spectral analysis and is able to induce significant immune 
      responses of macrophages. Herein, we further investigated the molecular mechanism 
      of CCP's immunomodulatory effects. The results indicate that CCP modulates the 
      TLR4/MyD88/p38 signaling pathway of macrophages, where TLR4 plays a crucial role 
      as verified on TLR4-deficient (TLR4(-/-)) bone marrow-derived macrophages (BMDMs) 
      and TLR4(-/-) mice. These findings provide a precise understanding of the 
      molecular mechanism of Cordyceps' immunomodulatory benefits.
CI  - Copyright (c) 2021. Published by Elsevier Ltd.
FAU - Zhang, Quanwei
AU  - Zhang Q
AD  - School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, China.
FAU - Liu, Man
AU  - Liu M
AD  - School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, China.
FAU - Li, Lifeng
AU  - Li L
AD  - School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, China.
FAU - Chen, Miaomiao
AU  - Chen M
AD  - School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, China.
FAU - Puno, Pema Tenzin
AU  - Puno PT
AD  - State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming 
      Institute of Botany, Chinese Academy of Sciences, Kunming, China.
FAU - Bao, Wanrong
AU  - Bao W
AD  - School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, China.
FAU - Zheng, Hongming
AU  - Zheng H
AD  - School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, China.
FAU - Wen, Xin
AU  - Wen X
AD  - School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, China.
FAU - Cheng, Huiyuan
AU  - Cheng H
AD  - School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, China.
FAU - Fung, Hauyee
AU  - Fung H
AD  - School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, China.
FAU - Wong, Tinlong
AU  - Wong T
AD  - School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, China.
FAU - Zhao, Zhongzhen
AU  - Zhao Z
AD  - School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, China.
FAU - Lyu, Aiping
AU  - Lyu A
AD  - School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, China. 
      Electronic address: aipinglu@hkbu.edu.hk.
FAU - Han, Quanbin
AU  - Han Q
AD  - School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, China. 
      Electronic address: simonhan@hkbu.edu.hk.
FAU - Sun, Handong
AU  - Sun H
AD  - State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming 
      Institute of Botany, Chinese Academy of Sciences, Kunming, China.
LA  - eng
PT  - Journal Article
DEP - 20210716
PL  - England
TA  - Carbohydr Polym
JT  - Carbohydrate polymers
JID - 8307156
RN  - 0 (Biomarkers)
RN  - 0 (Fungal Polysaccharides)
RN  - 0 (Immunologic Factors)
RN  - 0 (Interleukin-6)
RN  - 0 (Myd88 protein, mouse)
RN  - 0 (Myeloid Differentiation Factor 88)
RN  - 0 (Tlr2 protein, mouse)
RN  - 0 (Tlr4 protein, mouse)
RN  - 0 (Toll-Like Receptor 2)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (interleukin-6, mouse)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Animals
MH  - Biomarkers
MH  - Cordyceps/*chemistry
MH  - Fungal Polysaccharides/*pharmacology
MH  - Immunity/drug effects
MH  - Immunologic Factors/*pharmacology
MH  - Interleukin-6/metabolism
MH  - Macrophages/drug effects
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Moths
MH  - Myeloid Differentiation Factor 88/metabolism
MH  - RAW 264.7 Cells
MH  - Signal Transduction/*drug effects
MH  - Toll-Like Receptor 2/deficiency/metabolism
MH  - Toll-Like Receptor 4/deficiency
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - p38 Mitogen-Activated Protein Kinases/metabolism
OTO - NOTNLM
OT  - Cordyceps
OT  - Immune responses
OT  - Macrophage
OT  - Polysaccharide
OT  - TLR4/MyD88/p38
EDAT- 2021/08/09 06:00
MHDA- 2022/01/06 06:00
CRDT- 2021/08/08 20:24
PHST- 2021/06/03 00:00 [received]
PHST- 2021/06/28 00:00 [revised]
PHST- 2021/07/12 00:00 [accepted]
PHST- 2021/08/08 20:24 [entrez]
PHST- 2021/08/09 06:00 [pubmed]
PHST- 2022/01/06 06:00 [medline]
AID - S0144-8617(21)00830-4 [pii]
AID - 10.1016/j.carbpol.2021.118443 [doi]
PST - ppublish
SO  - Carbohydr Polym. 2021 Nov 1;271:118443. doi: 10.1016/j.carbpol.2021.118443. Epub 
      2021 Jul 16.