PMID- 34366684
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220425
IS  - 1179-5549 (Print)
IS  - 1179-5549 (Electronic)
IS  - 1179-5549 (Linking)
VI  - 15
DP  - 2021
TI  - High expression of cluster of differentiation 276 indicates poor prognosis in 
      glioma.
PG  - 11795549211032330
LID - 10.1177/11795549211032330 [doi]
LID - 11795549211032330
AB  - BACKGROUND: Glioma is the central nervous system tumor with the highest incidence 
      rate and the molecular detection of gliomas has been the focus of research. This 
      study aimed to investigate the guiding effect of cluster of differentiation 276 
      (CD276) expression on the clinical prognosis of glioma. METHODS: The TCGA and 
      CGGA databases were used to study whether CD 276 can be used as an independent 
      prognostic factor for gliomas. Immunohistochemistry was used to detect the 
      expression of CD276, isocitrate dehydrogenase-1 (IDH1), matrix metallopeptidase 9 
      (MMP9), p53, and Ki-67, and 1p/19q co-deletion was detected by fluorescence in 
      situ hybridization (FISH). The effects of CD276 RNA interference (RNAi) on cell 
      invasion, cell cycle and the expression of beta-catenin, tumor necrosis factor 
      receptor 1 (TNFR1), and MMP9 were observed. Furthermore, the biological effects 
      of CD276 gene knockout on intracranial transplanted tumors in nude mice were 
      studied. RESULTS: CD276 expression was positively correlated with the 
      extracellular matrix, collagen decomposition, and cell adhesion molecules. 
      Immunohistochemistry and FISH showed that CD276 expression positively correlated 
      with the glioma grade, p53 mutation, Ki-67 proliferation, and MMP9 expression; 
      however, it negatively correlated with IDH1 mutation, 1p/19q co-deletion, and the 
      survival rate. CD276 RNAi in U87 cells inhibited cell proliferation, migration, 
      and invasion, but had no effect on the cell cycle. CD276 inhibited the expression 
      of beta-catenin, TNFR1, and MMP9 in U87 cells at the mRNA and protein levels. In 
      vivo experiments showed that the tumor formation and invasion of the CD276 small 
      interfering RNA glioma cell line in nude mice were reduced and the survival time 
      was prolonged. CONCLUSIONS: The present study demonstrated that high expression 
      of CD276 in gliomas indicates a poor prognosis.
CI  - (c) The Author(s) 2021.
FAU - Li, Linchen
AU  - Li L
AD  - Department of Children Rehabilitation, Third Affiliated Hospital of Zhengzhou 
      University, Zhengzhou, P.R. China.
FAU - Zhang, Min
AU  - Zhang M
AD  - Department of Pathology, Second Affiliated Hospital of Zhengzhou University, 
      Zhengzhou, P.R. China.
FAU - Zhu, Dengna
AU  - Zhu D
AD  - Department of Children Rehabilitation, Third Affiliated Hospital of Zhengzhou 
      University, Zhengzhou, P.R. China.
FAU - Wang, Xinjun
AU  - Wang X
AUID- ORCID: 0000-0001-8139-2154
AD  - Department of Neurology, The Fifth Affiliated Hospital of Zhengzhou University, 
      Zhengzhou, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20210721
PL  - United States
TA  - Clin Med Insights Oncol
JT  - Clinical Medicine Insights. Oncology
JID - 101525771
PMC - PMC8299877
OTO - NOTNLM
OT  - CD276 expression
OT  - Gioma
OT  - prognosis
COIS- Declaration Of Conflicting Interests: The author(s) declared no potential 
      conflicts of interest with respect to the research, authorship, and/or 
      publication of this article.
EDAT- 2021/08/10 06:00
MHDA- 2021/08/10 06:01
PMCR- 2021/07/21
CRDT- 2021/08/09 06:26
PHST- 2020/11/16 00:00 [received]
PHST- 2021/06/23 00:00 [accepted]
PHST- 2021/08/09 06:26 [entrez]
PHST- 2021/08/10 06:00 [pubmed]
PHST- 2021/08/10 06:01 [medline]
PHST- 2021/07/21 00:00 [pmc-release]
AID - 10.1177_11795549211032330 [pii]
AID - 10.1177/11795549211032330 [doi]
PST - epublish
SO  - Clin Med Insights Oncol. 2021 Jul 21;15:11795549211032330. doi: 
      10.1177/11795549211032330. eCollection 2021.