PMID- 34374249
OWN - NLM
STAT- MEDLINE
DCOM- 20210811
LR  - 20210811
IS  - 1000-6834 (Print)
IS  - 1000-6834 (Linking)
VI  - 37
IP  - 3
DP  - 2021 May
TI  - [Effects of ERalpha gene overexpression on bone mineral density and calcium and 
      phosphorus metabolism inovariectomized osteoporosis mice].
PG  - 332-336
LID - 10.12047/j.cjap.6028.2021.002 [doi]
AB  - Objective: To investigate the effects of estrogen receptor alpha (ERalpha) gene 
      overexpression on bone metabolism and calcium and phosphorus metabolism in 
      ovariectomized osteoporosis mice, and to provide experimental basis for targeted 
      gene therapy of osteoporosis. Methods: Thirty SPF female mice were randomly 
      divided into sham operation group, model group and ERalpha overexpression group with 
      10 mice in each group. After the model was established, the ERalpha overexpression 
      group was transfected with recombinant adenovirus vector carrying mouse ERalpha gene 
      by intraspinal injection. The model group was transfected with empty virus, and 
      the sham operation group was not treated. The expression of ERalpha gene in bone 
      tissue of mice was detected by quantitative Real-time PCR (qRT-PCR). Bone mineral 
      density (BMD) of mouse femur was measured after modeling. Trabecular number 
      (Tb.N), trabecular thickness (Tb.Th), trabecular segregation (Tb.Sp), bone volume 
      fraction (BV/TV) and biomechanical strength of femur were measured by micro-CT 
      scanning. Serum levels of calcium (Ca), phosphorus (P), osteocalcin (BGP) and 
      alkaline phosphatase (ALP) were measured by automatic biochemical analyzer. The 
      expressions of tissue inhibitor of metalloproteinases 1 (TIMP-1) and monocyte 
      chemotactic protein 1 (MCP-1) in bone homogenate were detected by 
      Immunohistochemistry. Results: Compared with sham operation group, the expression 
      level of ERalpha gene in bone tissue of model group was decreased significantly, the 
      levels of BMD, BV/TV, Tb. Th, maximum load, rigidity coefficient, Ca and P were 
      decreased, while the levels of Tb. Sp, BGP and ALP were increased significantly 
      (P＜0.05). Compared with the sham operation group, the expression level of TIMP-1 
      protein in the bone tissue of the model group was significantly decreased, while 
      that of MCP-1 protein was increased, while that of the ERalpha overexpression group 
      was increased while that of MCP-1 was decreased (P＜0.05).The levels of ERalpha gene 
      expression, BMD, BV/TV, TB. Th, maximum load, rigidity coefficient, Ca and P in 
      the ERalpha overexpression group were significantly higher than those in the model 
      group, while Tb. Sp, BGP and ALP were significantly lower (P＜0.05). Compared with 
      the sham operation group, mean optical density of TIMP-1 in the bone tissue of 
      the model group was significantly decreased, while that of MCP-1 was 
      significantly increased, and that of the ERalpha overexpression group was 
      significantly increased while that of MCP-1 was significantly decreased (P＜0.05). 
      Conclusion: ERalpha gene overexpression can improve osteoporosis by regulating bone 
      mineral density, bone parameters, bone metabolism, calcium and phosphorus 
      metabolic indicators and the expression levels of TIMP-1 and MCP-1 in tissues.
FAU - Wang, Shan Xi
AU  - Wang SX
AD  - Xiangnan University Medical Imaging Examination and Rehabilitation College, 
      Chenzhou 423000.
FAU - Xie, Ju Ying
AU  - Xie JY
AD  - Department of Rehabilitation Medicine, Affiliated Hospital of Xiangnan 
      University, Chenzhou 423000.
FAU - Xie, Xing
AU  - Xie X
AD  - Department of Cardiovascular Medicine, Affiliated Hospital of Xiangnan 
      University, Chenzhou 423000, China.
FAU - Li, Shu Zhen
AU  - Li SZ
AD  - Xiangnan University Medical Imaging Examination and Rehabilitation College, 
      Chenzhou 423000.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhongguo Ying Yong Sheng Li Xue Za Zhi
JT  - Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = 
      Chinese journal of applied physiology
JID - 9426407
RN  - 0 (Estrogen Receptor alpha)
RN  - 27YLU75U4W (Phosphorus)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - *Bone Density
MH  - Calcium
MH  - Estrogen Receptor alpha/genetics
MH  - Female
MH  - Humans
MH  - Mice
MH  - *Osteoporosis
MH  - Ovariectomy
MH  - Phosphorus
MH  - Rats
MH  - Rats, Sprague-Dawley
OTO - NOTNLM
OT  - bone metabolism
OT  - calcium and phosphorus metabolism
OT  - mice
OT  - ovariectomized osteoporosis
OT  - estrogen receptor alpha
EDAT- 2021/08/11 06:00
MHDA- 2021/08/12 06:00
CRDT- 2021/08/10 07:01
PHST- 2021/08/10 07:01 [entrez]
PHST- 2021/08/11 06:00 [pubmed]
PHST- 2021/08/12 06:00 [medline]
AID - 10.12047/j.cjap.6028.2021.002 [doi]
PST - ppublish
SO  - Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2021 May;37(3):332-336. doi: 
      10.12047/j.cjap.6028.2021.002.