PMID- 34374260
OWN - NLM
STAT- MEDLINE
DCOM- 20210811
LR  - 20210811
IS  - 1000-6834 (Print)
IS  - 1000-6834 (Linking)
VI  - 37
IP  - 4
DP  - 2021 Jul
TI  - [Protective effects of total saponins of Codonopsis on ulcerative colitis induced 
      by TNBS in rats and its mechanism].
PG  - 397-401
LID - 10.12047/j.cjap.6051.2021.032 [doi]
AB  - Objective: To study the protective effects and mechanisms of total saponins of 
      Codonopsis (TSC) on ulcerative colitis in rats. Methods: Fifty male Wistar rats 
      were randomly divided into 5 groups: control group, model group, 
      salazosulfadiazine (SASP) positive control group (0.3 g/kg), TSC high- and 
      low-dose experimental groups(1.2, 0.4 g/kg). UC rat model was established by 
      trinitrobenzene sulfonic acid (TNBS)/ ethanol enema. After administration for 21 
      days, the rats' symptoms and signs, disease activity index (DAI), colonic mucosal 
      injury index (CMDI) and colonic tissue morphology were observed. The contents of 
      superoxide dismutase (SOD), malondialdehyde (MDA), inflammatory cytokines 
      interleukin-6 (IL-6), interleukin-10 (IL-10) and tumor necrosis factor (TNF-alpha) in 
      colon tissues were determined. Protein expression of nuclear nuclear 
      transcription factor-kappaB (NF-kappaB) in colon tissues was detected. Finally, the 
      effect of TCS therapy was evaluated. Results: Compared with the control group, 
      the DAI and CMDI scores of the rats in the model group were increased 
      significantly, meanwhile the colonic mucosa was seriously damaged, indicating 
      that the model was successful. Compared with the model group, the TSC high and 
      low dose groups could significantly reduce the DAI and CMDI score (P＜0.05) and 
      improve the colonic mucosa form. TSC also could increase the SOD activity and 
      decrease MDA content in colon tissues(P＜0.05), while inhibit the levels of IL-6 
      and TNF-alpha mRNA in the colon tissues and promote the expression of IL-10 mRNA 
      (P＜0.01). At the same time, TSC reduced the expressions of NF-kappaB protein in the 
      colon (P＜0.01). The TSC high-dose group was superior to the low-dose group 
      (P＜0.05). Conclusion: TSC has significant protective effects on ulcerative 
      colonic mucosal damage in UC rats, and there is a dose-dependent relationship; 
      its mechanism may be related to anti-lipid peroxidation and inhibiting the NF-kappaB 
      signaling pathway to regulate the release of inflammatory factors.
FAU - Liu, Xue Feng
AU  - Liu XF
AD  - Pharmacy Collage of Gansu University of Chinese Medicine, Lanzhou 730000.
AD  - Key Laboratory of Chinese and Tibetan Medicine Chemistry and Quality Research in 
      Gansu Province, Lanzhou 730000.
FAU - Qiao, Jing
AU  - Qiao J
AD  - Pharmacy Collage of Gansu University of Chinese Medicine, Lanzhou 730000.
FAU - Gao, Jian de
AU  - Gao J
AD  - Pharmacy Collage of Gansu University of Chinese Medicine, Lanzhou 730000.
AD  - Key Laboratory of Traditional Chinese Medicine Quality and Standard in Gansu 
      Province, Lanzhou 730000, China.
FAU - Chen, Zheng Jun
AU  - Chen ZJ
AD  - Key Laboratory of Chinese and Tibetan Medicine Chemistry and Quality Research in 
      Gansu Province, Lanzhou 730000.
FAU - Liu, Xiong
AU  - Liu X
AD  - Pharmacy Collage of Gansu University of Chinese Medicine, Lanzhou 730000.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhongguo Ying Yong Sheng Li Xue Za Zhi
JT  - Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = 
      Chinese journal of applied physiology
JID - 9426407
RN  - 0 (NF-kappa B)
RN  - 0 (Saponins)
RN  - 8T3HQG2ZC4 (Trinitrobenzenesulfonic Acid)
SB  - IM
MH  - Animals
MH  - *Codonopsis
MH  - *Colitis, Ulcerative/chemically induced/drug therapy
MH  - Colon
MH  - Male
MH  - NF-kappa B
MH  - Rats
MH  - Rats, Wistar
MH  - *Saponins/pharmacology
MH  - Trinitrobenzenesulfonic Acid
OTO - NOTNLM
OT  - NF-kappaB
OT  - cytokine
OT  - rat
OT  - total saponins of codonopsis
OT  - ulcerative colitis
EDAT- 2021/08/11 06:00
MHDA- 2021/08/12 06:00
CRDT- 2021/08/10 07:01
PHST- 2021/08/10 07:01 [entrez]
PHST- 2021/08/11 06:00 [pubmed]
PHST- 2021/08/12 06:00 [medline]
AID - 10.12047/j.cjap.6051.2021.032 [doi]
PST - ppublish
SO  - Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2021 Jul;37(4):397-401. doi: 
      10.12047/j.cjap.6051.2021.032.