PMID- 34376787
OWN - NLM
STAT- MEDLINE
DCOM- 20211108
LR  - 20211108
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 11
IP  - 1
DP  - 2021 Aug 10
TI  - Rab1A promotes cell proliferation and migration by upregulating Gli1 in 
      colorectal cancer.
PG  - 16243
LID - 10.1038/s41598-021-95798-7 [doi]
LID - 16243
AB  - Rab1A, as a highly conserved small guanosine triphosphatase (GTPase), plays 
      contentious roles in different types of cancers. The role of Rab1A in colorectal 
      cancer (CRC) has been described in previous studies, but the molecular mechanisms 
      of Rab1A in CRC remain far from being addressed. In the present study, we found 
      that Rab1A expression was significantly upregulated in CRC tissues and increased 
      Rab1A expression correlated with tumor size, lymph node metastasis (LNM) and 
      tumor-node-metastasis (TNM) stage of CRC patients. We also found that Rab1A 
      exerts its promotive effect on CRC cell proliferation, migration and EMT 
      progress. Further mechanistic experiments showed that glioma-associated 
      oncogene-1 (Gli1), as a key transcriptional factor of the Hedgehog pathway, was 
      implicated in Rab1A-mediated regulation of CRC cell proliferation and migration. 
      In addition, Rab1A upregulated Gli1 expression through Smoothened homolog 
      (SMO)-independent pathway. Finally, Rab1A activated mechanistic target of 
      rapamycin (mTOR) signaling in CRC cells. Collectively, our results define Rab1A 
      as a novel regulator of Gli1 to promote CRC cell proliferation and migration, and 
      suggest that the Rab1A/mTOR/Gli1 axis may serve as a promising therapeutic target 
      for the treatment of CRC.
CI  - (c) 2021. The Author(s).
FAU - Peng, Chaozhong
AU  - Peng C
AD  - Department of General Surgery, Suzhou Wuzhong People's Hospital, No. 61 Dongwu 
      North Road, Suzhou, 215128, Jiangsu, China.
FAU - Li, Xiao
AU  - Li X
AD  - Department of General Surgery, Suzhou Wuzhong People's Hospital, No. 61 Dongwu 
      North Road, Suzhou, 215128, Jiangsu, China.
FAU - Ye, Zhixue
AU  - Ye Z
AD  - Department of General Surgery, Suzhou Wuzhong People's Hospital, No. 61 Dongwu 
      North Road, Suzhou, 215128, Jiangsu, China.
FAU - Wu, Wenqing
AU  - Wu W
AD  - Department of General Surgery, Suzhou Wuzhong People's Hospital, No. 61 Dongwu 
      North Road, Suzhou, 215128, Jiangsu, China. wuwenqing2021@163.com.
LA  - eng
PT  - Journal Article
DEP - 20210810
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (GLI1 protein, human)
RN  - 0 (RAB1A protein, human)
RN  - 0 (Zinc Finger Protein GLI1)
RN  - EC 3.6.5.2 (rab1 GTP-Binding Proteins)
SB  - IM
MH  - Apoptosis
MH  - Biomarkers, Tumor/genetics/*metabolism
MH  - *Cell Movement
MH  - *Cell Proliferation
MH  - Colorectal Neoplasms/genetics/metabolism/*pathology
MH  - *Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Prognosis
MH  - Survival Rate
MH  - Tumor Cells, Cultured
MH  - Zinc Finger Protein GLI1/genetics/*metabolism
MH  - rab1 GTP-Binding Proteins/genetics/*metabolism
PMC - PMC8355269
COIS- The authors declare no competing interests.
EDAT- 2021/08/12 06:00
MHDA- 2021/11/09 06:00
PMCR- 2021/08/10
CRDT- 2021/08/11 06:25
PHST- 2021/05/23 00:00 [received]
PHST- 2021/07/31 00:00 [accepted]
PHST- 2021/08/11 06:25 [entrez]
PHST- 2021/08/12 06:00 [pubmed]
PHST- 2021/11/09 06:00 [medline]
PHST- 2021/08/10 00:00 [pmc-release]
AID - 10.1038/s41598-021-95798-7 [pii]
AID - 95798 [pii]
AID - 10.1038/s41598-021-95798-7 [doi]
PST - epublish
SO  - Sci Rep. 2021 Aug 10;11(1):16243. doi: 10.1038/s41598-021-95798-7.