PMID- 34378805
OWN - NLM
STAT- MEDLINE
DCOM- 20220504
LR  - 20220504
IS  - 1097-4652 (Electronic)
IS  - 0021-9541 (Linking)
VI  - 237
IP  - 1
DP  - 2022 Jan
TI  - Merlin functions as a critical regulator in Staphylococcus aureus-induced 
      osteomyelitis.
PG  - 815-823
LID - 10.1002/jcp.30550 [doi]
AB  - Merlin is known as a tumor suppressor, while its role in osteomyelitis remains 
      unclear. This study aimed to investigate the role of Merlin in Staphylococcus 
      aureus-induced osteomyelitis and its underlying mechanisms. S. aureus-induced 
      osteomyelitis mouse model was established in Merlin(fl/fl) Lyz2(cre/+) and 
      Merlin(fl/fl) Lyz2(+/+) mice. Bone marrow-derived macrophages (BMDMs) were 
      isolated and stimulated by lipopolysaccharide (LPS). Bioassays, including 
      quantitative reverse transcription polymerase chain reaction (qRT-PCR), Western 
      blot analysis, and enzyme-linked immunosorbent assays, were conducted to 
      determine the levels of target genes or proteins. Immunoprecipitation was applied 
      to determine the interactions between proteins. DCAF1(fl/fl) mice were further 
      crossed with Lyz2-Cre mice to establish myeloid cell conditional knockout mice 
      (DCAF1(fl/fl) Lyz2(cre/+) ). It was found that the level of Merlin was elevated 
      in patients with osteomyelitis and S. aureus-infected BMDMs. Merlin deficiency in 
      macrophages suppressed the production of inflammatory cytokines and ameliorated 
      the symptoms of osteomyelitis induced by S. aureus. Merlin deficiency in 
      macrophages also suppressed the production of proinflammatory cytokines in BMDMs 
      induced by LPS. The inhibitory effects of Merlin deficiency on the inflammatory 
      response were associated with DDB1-Cul4-associated factor 1 (DCAF1). In summary, 
      Merlin deficiency ameliorates S. aureus-induced osteomyelitis through the 
      regulation of DCAF1.
CI  - (c) 2021 Wiley Periodicals LLC.
FAU - Zhou, Zubin
AU  - Zhou Z
AD  - Department of Orthopaedic Surgery, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, Shanghai, China.
FAU - Chen, Yuanliang
AU  - Chen Y
AD  - Department of Orthopaedic Surgery, Haikou Orthopedic and Diabetes Hospital of 
      Shanghai Sixth People's Hospital, Haikou, Hainan, China.
FAU - Min, Hong Sung
AU  - Min HS
AD  - Department of Orthopaedic Surgery, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, Shanghai, China.
FAU - Wan, Yongbai
AU  - Wan Y
AD  - Department of Orthopaedic Surgery, Haikou Orthopedic and Diabetes Hospital of 
      Shanghai Sixth People's Hospital, Haikou, Hainan, China.
FAU - Shan, Haojie
AU  - Shan H
AD  - Department of Orthopaedic Surgery, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, Shanghai, China.
FAU - Lin, Yiwei
AU  - Lin Y
AD  - Department of Orthopaedic Surgery, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, Shanghai, China.
FAU - Xia, Wenyang
AU  - Xia W
AD  - Department of Orthopaedic Surgery, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, Shanghai, China.
FAU - Yin, Fuli
AU  - Yin F
AD  - Department of Orthopaedic Surgery, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, Shanghai, China.
FAU - Jiang, Chaolai
AU  - Jiang C
AD  - Department of Orthopaedic Surgery, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, Shanghai, China.
FAU - Yu, Xiaowei
AU  - Yu X
AUID- ORCID: 0000-0002-8235-4907
AD  - Department of Orthopaedic Surgery, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, Shanghai, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210811
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
RN  - 0 (Cytokines)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Neurofibromin 2)
SB  - IM
MH  - Animals
MH  - Cytokines
MH  - Humans
MH  - Lipopolysaccharides/pharmacology
MH  - Mice
MH  - Neurofibromin 2/genetics/metabolism
MH  - *Osteomyelitis
MH  - *Staphylococcal Infections
MH  - Staphylococcus aureus/metabolism
OTO - NOTNLM
OT  - DCAF1
OT  - Merlin
OT  - Staphylococcus aureus
OT  - inflammatory response
OT  - osteomyelitis
EDAT- 2021/08/12 06:00
MHDA- 2022/05/06 06:00
CRDT- 2021/08/11 09:08
PHST- 2021/07/12 00:00 [revised]
PHST- 2021/04/19 00:00 [received]
PHST- 2021/08/02 00:00 [accepted]
PHST- 2021/08/12 06:00 [pubmed]
PHST- 2022/05/06 06:00 [medline]
PHST- 2021/08/11 09:08 [entrez]
AID - 10.1002/jcp.30550 [doi]
PST - ppublish
SO  - J Cell Physiol. 2022 Jan;237(1):815-823. doi: 10.1002/jcp.30550. Epub 2021 Aug 
      11.