PMID- 34378992
OWN - NLM
STAT- MEDLINE
DCOM- 20210921
LR  - 20220902
IS  - 1522-1563 (Electronic)
IS  - 0363-6143 (Print)
IS  - 0363-6143 (Linking)
VI  - 321
IP  - 3
DP  - 2021 Sep 1
TI  - Class A scavenger receptor-1/2 facilitates the uptake of bovine milk exosomes in 
      murine bone marrow-derived macrophages and C57BL/6J mice.
PG  - C607-C614
LID - 10.1152/ajpcell.00222.2021 [doi]
AB  - Bovine milk exosomes (BMEs) are being explored in drug delivery despite their 
      rapid elimination by macrophages. We aimed at identifying the BME transporter in 
      murine bone marrow-derived macrophages (BMDMs). Fluorophore-labeled BMEs were 
      used in transport studies in BMDMs from C57BL/6J and class A scavenger receptor 
      type 1/2 (CASR-1/2) knockout mice and tissue accumulation in macrophage-depleted 
      C57BL/6J mice. Parametric and nonparametric statistics tests for pairwise and 
      multiple comparisons were used. Chemical inhibitors of phagocytosis by 
      cytochalasin D led to a 69 +/- 18% decrease in BME uptake compared with controls (P 
      < 0.05), whereas inhibitors of endocytic pathways other than phagocytosis had a 
      modest effect on uptake (P > 0.05). Inhibitors of class A scavenger receptors 
      (CASRs) including CASR-1/2 caused a 70% decrease in BME uptake (P < 0.05). The 
      uptake of BMEs by BMDMs from CASR-1/2 knockout mice was smaller by 58 +/- 23% 
      compared with wild-type controls (P < 0.05). Macrophage depletion by clodronate 
      caused a more than 44% decrease in BME uptake in the spleen and lungs (P < 0.05), 
      whereas the decrease observed in liver was not statistically significant. In 
      conclusion, CASR-1/2 facilitates the uptake of BMEs in BMDMs and C57BL/6J mice.
FAU - Khanam, Afsana
AU  - Khanam A
AUID- ORCID: 0000-0001-9813-5138
AD  - Department of Nutrition and Health Sciences, University of Nebraska-Lincoln, 
      Lincoln, Nebraska.
FAU - Yu, Jiujiu
AU  - Yu J
AD  - Department of Nutrition and Health Sciences, University of Nebraska-Lincoln, 
      Lincoln, Nebraska.
FAU - Zempleni, Janos
AU  - Zempleni J
AUID- ORCID: 0000-0001-5492-4661
AD  - Department of Nutrition and Health Sciences, University of Nebraska-Lincoln, 
      Lincoln, Nebraska.
LA  - eng
SI  - figshare/10.6084/m9.figshare.14763363.v5
GR  - P20 GM104320/GM/NIGMS NIH HHS/United States
GR  - P20GM104320/HHS | NIH | National Institute of General Medical Sciences (NIGMS)/
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20210811
PL  - United States
TA  - Am J Physiol Cell Physiol
JT  - American journal of physiology. Cell physiology
JID - 100901225
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Msr1 protein, mouse)
RN  - 0 (Protein Isoforms)
RN  - 0 (Scavenger Receptors, Class A)
RN  - 0813BZ6866 (Clodronic Acid)
RN  - 22144-77-0 (Cytochalasin D)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Clodronic Acid/pharmacology
MH  - Cytochalasin D/pharmacology
MH  - Endocytosis/drug effects
MH  - Exosomes/chemistry/*metabolism
MH  - Female
MH  - Fluorescent Dyes/chemistry
MH  - Gene Expression
MH  - Liver/drug effects/metabolism
MH  - Lung/drug effects/metabolism
MH  - Macrophages/cytology/drug effects/*metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Milk/*chemistry
MH  - Phagocytosis/drug effects
MH  - Protein Isoforms/deficiency/genetics
MH  - Scavenger Receptors, Class A/deficiency/*genetics
MH  - Spleen/drug effects/metabolism
MH  - Staining and Labeling/methods
PMC - PMC8461812
OTO - NOTNLM
OT  - bone marrow-derived macrophages
OT  - bovine
OT  - class A scavenger receptors
OT  - milk exosomes
OT  - phagocytosis
COIS- J.Z. serves as a consultant for PureTech Health, Inc. None of the other authors 
      has any conflicts of interest, financial or otherwise, to disclose.
EDAT- 2021/08/12 06:00
MHDA- 2021/09/22 06:00
PMCR- 2022/09/01
CRDT- 2021/08/11 12:12
PHST- 2021/08/12 06:00 [pubmed]
PHST- 2021/09/22 06:00 [medline]
PHST- 2021/08/11 12:12 [entrez]
PHST- 2022/09/01 00:00 [pmc-release]
AID - C-00222-2021 [pii]
AID - 10.1152/ajpcell.00222.2021 [doi]
PST - ppublish
SO  - Am J Physiol Cell Physiol. 2021 Sep 1;321(3):C607-C614. doi: 
      10.1152/ajpcell.00222.2021. Epub 2021 Aug 11.