PMID- 34380794
OWN - NLM
STAT- MEDLINE
DCOM- 20210820
LR  - 20220425
IS  - 0971-5916 (Print)
IS  - 0975-9174 (Electronic)
IS  - 0971-5916 (Linking)
VI  - 153
IP  - 4
DP  - 2021 Apr
TI  - Role of cytogenetic abnormalities detected by fluorescence in situ hybridization 
      as a prognostic marker: Pathogenesis & clinical course in patients with B-chronic 
      lymphocytic leukaemia.
PG  - 475-483
LID - 10.4103/ijmr.IJMR_2257_18 [doi]
AB  - BACKGROUND & OBJECTIVES: B-cell chronic lymphocytic leukaemia (B-CLL) is one of 
      the most common forms of adult leukaemia, with a highly variable clinical course. 
      Specific chromosomal and genetic aberrations are used clinically to predict 
      prognosis, independent from conventional clinical markers. Molecular cytogenetic 
      methods such as fluorescence in situ hybridization (FISH) detect aberrations in 
      up to 80 per cent B-CLL patients. This study was conducted to score the 
      frequencies of recurrent aberrations, i.e., del(13q14), trisomy 12, del(11q22), 
      del(17p13), del(6q21) and IgH (immunoglobulin heavy chain) translocations and to 
      understand their role in prognostication and risk stratification. METHODS: FISH 
      studies were performed on bone marrow aspirate or peripheral blood of 280 
      patients using commercially available disease-specific probe set. The data were 
      correlated with clinical and haematological parameters such as low haemoglobin, 
      splenomegaly and lymphadenopathy. RESULTS: Chromosomal aberrations were detected 
      in 79 per cent of patients, with del(13q14) (57%) as the most common cytogenetic 
      aberration, followed by trisomy 12 (27%), del(11q22) (22%), t(14q32) (19%), 
      del(17p13) (18%) and del(6q21) (9%). Single or in coexistence with other 
      aberration del(13q14) had a favourable outcome in comparison to del(11q22), 
      t(14q32), del(17p13) and del(6q21) which were associated with advanced stages of 
      the disease. Trisomy 12 had a variable clinical course. INTERPRETATION & 
      CONCLUSIONS: FISH was found to be a sensitive and efficient technique in 
      detecting the prevalence of recurrent cytogenetic abnormalities. Each of these 
      aberrations is an important independent predictor of disease progression and 
      survival which aids in designing risk-adapted treatment strategies for better 
      disease management.
FAU - Shetty, Dhanlaxmi
AU  - Shetty D
AD  - Department of Cancer Cytogenetics, Advanced Centre for Training, Research & 
      Education in Cancer, Tata Memorial Centre, Kharghar, Mumbai, Maharashtra, India.
FAU - Jain, Hemani
AU  - Jain H
AD  - Department of Cancer Cytogenetics, Advanced Centre for Training, Research & 
      Education in Cancer, Tata Memorial Centre, Kharghar, Mumbai, Maharashtra, India.
FAU - Rohil, Yogita
AU  - Rohil Y
AD  - Department of Cancer Cytogenetics, Advanced Centre for Training, Research & 
      Education in Cancer, Tata Memorial Centre, Kharghar, Mumbai, Maharashtra, India.
FAU - Khattry, Navin
AU  - Khattry N
AD  - Department of Medical Oncology, Advanced Centre for Training, Research & 
      Education in Cancer, Tata Memorial Centre, Kharghar; Homi Bhabha National 
      Institute (HBNI), Mumbai, Maharashtra, India.
FAU - Sengar, Manju
AU  - Sengar M
AD  - Department of Medical Oncology, Tata Memorial Hospital, Tata Memorial Centre, 
      Parel; Homi Bhabha National Institute (HBNI), Mumbai, Maharashtra, India.
FAU - Bagal, Bhausaheb
AU  - Bagal B
AD  - Department of Medical Oncology, Tata Memorial Hospital, Tata Memorial Centre, 
      Parel; Homi Bhabha National Institute (HBNI), Mumbai, Maharashtra, India.
FAU - Jain, Hasmukh
AU  - Jain H
AD  - Department of Medical Oncology, Tata Memorial Hospital, Tata Memorial Centre, 
      Parel; Homi Bhabha National Institute (HBNI), Mumbai, Maharashtra, India.
FAU - Gokarn, Anant
AU  - Gokarn A
AD  - Department of Medical Oncology, Advanced Centre for Training, Research & 
      Education in Cancer, Tata Memorial Centre, Kharghar; Homi Bhabha National 
      Institute (HBNI), Mumbai, Maharashtra, India.
FAU - Punatar, Sachin
AU  - Punatar S
AD  - Department of Medical Oncology, Advanced Centre for Training, Research & 
      Education in Cancer, Tata Memorial Centre, Kharghar; Homi Bhabha National 
      Institute (HBNI), Mumbai, Maharashtra, India.
FAU - Avinash Bonda, Venkata Naga
AU  - Avinash Bonda VN
AD  - Department of Medical Oncology, Tata Memorial Hospital, Tata Memorial Centre, 
      Parel; Homi Bhabha National Institute (HBNI), Mumbai, Maharashtra, India.
FAU - Subramanian, P G
AU  - Subramanian PG
AD  - Department of Hematopathology, Advanced Centre for Training, Research & Education 
      in Cancer, Tata Memorial Centre, Kharghar; Homi Bhabha National Institute (HBNI), 
      Mumbai, Maharashtra, India.
LA  - eng
PT  - Journal Article
PL  - India
TA  - Indian J Med Res
JT  - The Indian journal of medical research
JID - 0374701
SB  - IM
MH  - Chromosome Aberrations
MH  - Cytogenetic Analysis
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - *Leukemia, Lymphocytic, Chronic, B-Cell/diagnosis/genetics
MH  - Prognosis
PMC - PMC8354055
OTO - NOTNLM
OT  - B-cell chronic lymphocytic leukaemia
OT  - chromosomal aberration
OT  - fluorescence in situ
OT  - hybridization
OT  - lactate dehydrogenase
OT  - prognosis
OT  - time-to-first treatment
OT  - treatment-free survival
COIS- None
EDAT- 2021/08/13 06:00
MHDA- 2021/08/21 06:00
PMCR- 2021/04/01
CRDT- 2021/08/12 05:43
PHST- 2021/08/12 05:43 [entrez]
PHST- 2021/08/13 06:00 [pubmed]
PHST- 2021/08/21 06:00 [medline]
PHST- 2021/04/01 00:00 [pmc-release]
AID - IndianJMedRes_2021_153_4_475_322936 [pii]
AID - IJMR-153-475 [pii]
AID - 10.4103/ijmr.IJMR_2257_18 [doi]
PST - ppublish
SO  - Indian J Med Res. 2021 Apr;153(4):475-483. doi: 10.4103/ijmr.IJMR_2257_18.