PMID- 34381025
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210831
IS  - 2058-7716 (Print)
IS  - 2058-7716 (Electronic)
IS  - 2058-7716 (Linking)
VI  - 7
IP  - 1
DP  - 2021 Aug 11
TI  - Human umbilical cord mesenchymal stem cells-derived extracellular vesicles 
      facilitate the repair of spinal cord injury via the miR-29b-3p/PTEN/Akt/mTOR 
      axis.
PG  - 212
LID - 10.1038/s41420-021-00572-3 [doi]
LID - 212
AB  - Spinal cord injury (SCI) is a salient traumatic disease that often leads to 
      permanent disability, and motor and sensory impairments. Human umbilical cord 
      mesenchymal stem cells (HucMSCs) have a wide application prospect in the 
      treatment of SCI. This study explored the repair effect of HucMSCs-derived 
      extracellular vesicles (HucMSCs-EVs) on SCI. HucMSCs and HucMSCs-EVs were 
      cultured and identified. The rat model of SCI was established, and SCI rats were 
      treated with HucMSCs-EVs. The motor function of SCI rats and morphology of spinal 
      cord tissues were evaluated. Levels of NeuN, GFAP, and NF200 in spinal cord 
      tissues were detected and cell apoptosis was measured. SCI rats were treated with 
      EVs extracted from miR-29b-3p inhibitor-transfected HucMSCs. The downstream gene 
      and pathway of miR-29b-3p were examined. HucMSCs-EVs-treated rats showed obvious 
      motor function recovery and reduced necrosis, nuclear pyknosis, and cavity. 
      HucMSCs-EVs alleviated spinal cord neuronal injury. miR-29b-3p was poorly 
      expressed in SCI tissues, but highly expressed in EVs and SCI rats treated with 
      EVs. miR-29b-3p targeted PTEN. Inhibition of miR-29b-3p or overexpression of PTEN 
      reversed the repair effect of EVs on SCI. EVs activated the AKT/mTOR pathway via 
      the miR-29b-3p/PTEN. In conclusion, HucMSCs-EVs reduced pathological changes, 
      improved motor function, and promoted nerve function repair in SCI rats via the 
      miR-29b-3p/PTEN/Akt/mTOR axis.
CI  - (c) 2021. The Author(s).
FAU - Xiao, Xiao
AU  - Xiao X
AD  - Department of Spine Surgery, The Xiangya Hospital of Central-South University, 
      Changsha, China.
FAU - Li, Weiwei
AU  - Li W
AD  - Department of Spine Surgery, The Xiangya Hospital of Central-South University, 
      Changsha, China.
FAU - Rong, Dingchao
AU  - Rong D
AD  - Department of Spine Surgery, The Xiangya Hospital of Central-South University, 
      Changsha, China.
FAU - Xu, Zhenchao
AU  - Xu Z
AD  - Department of Spine Surgery, The Xiangya Hospital of Central-South University, 
      Changsha, China.
FAU - Zhang, Zhen
AU  - Zhang Z
AD  - Department of Spine Surgery, The Xiangya Hospital of Central-South University, 
      Changsha, China.
FAU - Ye, Hongru
AU  - Ye H
AD  - Department of Spine Surgery, The Xiangya Hospital of Central-South University, 
      Changsha, China.
FAU - Xie, Liqiong
AU  - Xie L
AD  - Department of Spine Surgery, The Xiangya Hospital of Central-South University, 
      Changsha, China.
FAU - Wu, Yunqi
AU  - Wu Y
AD  - Hunan Engineering Laboratory of Advanced Artificial Osteo-materials, Changsha, 
      China.
FAU - Zhang, Yilu
AU  - Zhang Y
AD  - Hunan Engineering Laboratory of Advanced Artificial Osteo-materials, Changsha, 
      China.
FAU - Wang, Xiyang
AU  - Wang X
AD  - Department of Spine Surgery, The Xiangya Hospital of Central-South University, 
      Changsha, China. 188102123@csu.edu.cn.
AD  - Hunan Engineering Laboratory of Advanced Artificial Osteo-materials, Changsha, 
      China. 188102123@csu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20210811
PL  - United States
TA  - Cell Death Discov
JT  - Cell death discovery
JID - 101665035
PMC - PMC8357833
COIS- The authors declare no competing interests.
EDAT- 2021/08/13 06:00
MHDA- 2021/08/13 06:01
PMCR- 2021/08/11
CRDT- 2021/08/12 05:49
PHST- 2020/12/29 00:00 [received]
PHST- 2021/07/07 00:00 [accepted]
PHST- 2021/06/15 00:00 [revised]
PHST- 2021/08/12 05:49 [entrez]
PHST- 2021/08/13 06:00 [pubmed]
PHST- 2021/08/13 06:01 [medline]
PHST- 2021/08/11 00:00 [pmc-release]
AID - 10.1038/s41420-021-00572-3 [pii]
AID - 572 [pii]
AID - 10.1038/s41420-021-00572-3 [doi]
PST - epublish
SO  - Cell Death Discov. 2021 Aug 11;7(1):212. doi: 10.1038/s41420-021-00572-3.