PMID- 34384940 OWN - NLM STAT- MEDLINE DCOM- 20220114 LR - 20220114 IS - 1618-0372 (Electronic) IS - 0065-1281 (Linking) VI - 123 IP - 6 DP - 2021 Sep TI - Seasonal expression and distribution of kisspeptin1 (kiss1) in the ovary and testis of freshwater catfish, Clarias batrachus: A putative role in steroidogenesis. PG - 151766 LID - S0065-1281(21)00088-X [pii] LID - 10.1016/j.acthis.2021.151766 [doi] AB - The central role of kisspeptin (kiss) in mammalian reproduction is well established; however, its intra-gonadal role is poorly addressed. Moreover, studies investigating intra-gonadal role of kiss in fish reproduction are scanty, contradictory and inconclusive. The expression of kiss1 mRNA has been detected in the fish brain, and functionally attributed to the regulation of reproduction, feeding and behavior. The kiss1 mRNA has also been demonstrated in tissues other than the brain in some studies, but its cellular distribution and role at the tissue level have not been adequately addressed in fish. Therefore, an attempt was made in the present study to localize kiss1 in gonadal cells of the freshwater catfish, Clarias batrachus. This study reports the presence of kiss1 in the theca cells and granulosa cells of the ovarian oocytes and interstitial cells in the testis of the catfish. The role of kiss1 in the ovary and testis of the catfish was also investigated using kiss1 receptor (kiss1r) antagonist (p234). The p234 treatment decreased the production of 17beta-estradiol in ovary and testosterone in the testis by lowering the activities of 3beta-hydroxysteroid dehydrogenase and 17beta-hydroxysteroid dehydrogenase under both, in vivo as well as in vitro conditions. The p234 treatment also arrested the progression of oogenesis, as evident from the low number of advancing/advanced oocytes in the treated ovary in comparison to the control ovary. It also reduced the area and perimeter of the seminiferous tubules in the treated catfish testis. Thus, our findings suggest that kiss is involved in the regulation of gonadal steroidogenesis, independent of known endocrine/ autocrine/ paracine regulators, and thereby it accelerates gametogenic processes in the freshwater catfish. CI - Copyright (c) 2021 Elsevier GmbH. All rights reserved. FAU - Singh, Ankur AU - Singh A AD - Fish Endocrinology Laboratory, Department of Zoology, Institute of Science, Banaras Hindu University, Varanasi, India. FAU - Lal, Bechan AU - Lal B AD - Fish Endocrinology Laboratory, Department of Zoology, Institute of Science, Banaras Hindu University, Varanasi, India. Electronic address: lalbhu@yahoo.co.in. FAU - Parhar, Ishwar S AU - Parhar IS AD - Brain Research Institute, School of Medicine and Health Sciences, Monash University, Sunway Campus, Malaysia. FAU - Millar, Robert P AU - Millar RP AD - Centre for Neuroendocrinology, Department of Immunology, University of Pretoria, Pretoria and Department of Integrative Biomedical Sciences, Institute of Infectious Diseases and Molecular Medicine, University of Cape Town, South Africa. LA - eng PT - Journal Article DEP - 20210809 PL - Germany TA - Acta Histochem JT - Acta histochemica JID - 0370320 RN - 0 (Kisspeptins) SB - IM MH - Animals MH - Catfishes/genetics/*metabolism MH - Female MH - *Gene Expression Regulation MH - Kisspeptins/*biosynthesis/genetics MH - Male MH - Ovary/*metabolism MH - *Seasons MH - Testis/*metabolism OTO - NOTNLM OT - 17beta-HSD OT - 3beta-HSD OT - Gametogenesis OT - Kisspeptin OT - Sex steroids EDAT- 2021/08/14 06:00 MHDA- 2022/01/15 06:00 CRDT- 2021/08/13 05:59 PHST- 2021/01/20 00:00 [received] PHST- 2021/07/27 00:00 [revised] PHST- 2021/08/02 00:00 [accepted] PHST- 2021/08/14 06:00 [pubmed] PHST- 2022/01/15 06:00 [medline] PHST- 2021/08/13 05:59 [entrez] AID - S0065-1281(21)00088-X [pii] AID - 10.1016/j.acthis.2021.151766 [doi] PST - ppublish SO - Acta Histochem. 2021 Sep;123(6):151766. doi: 10.1016/j.acthis.2021.151766. Epub 2021 Aug 9.