PMID- 34385105
OWN - NLM
STAT- MEDLINE
DCOM- 20220106
LR  - 20220106
IS  - 1950-6007 (Electronic)
IS  - 0753-3322 (Linking)
VI  - 142
DP  - 2021 Oct
TI  - Panax notoginseng saponins reduces the cisplatin-induced acute renal injury by 
      increasing HIF-1alpha/BNIP3 to inhibit mitochondrial apoptosis pathway.
PG  - 111965
LID - S0753-3322(21)00747-2 [pii]
LID - 10.1016/j.biopha.2021.111965 [doi]
AB  - Cisplatin (CDDP) may induce apoptosis of renal tubular epithelial cells (RTEC) 
      and cause CDDP-induced acute kidney injury (CAKI) during cancer treatment, but 
      yet lack of preventive measures and effective treatment. As a new Chinese herbal 
      preparation, Panax notoginseng saponins (PNS) has been found to mitigate 
      CDDP-induced CAKI through elevating the expression of HIF-1alpha in the rat model, 
      according to the data from our previous works. However, the underlying link 
      between HIF-1alpha and apoptosis has not been well elucidated. The current study as a 
      follow-up work, was aimed to reveal if PNS improves CAKI through HIF-1alpha-dependent 
      apoptosis. A stably HIF-1alpha-knockdown human proximal tubular epithelial cell 
      (HK-2) line was established by transfecting a HIF-1alpha-siRNA into HK-2 cells. Cell 
      viability, mitochondrial function, cell apoptosis ratio and the expression of 
      apoptosis-associated proteins (Cyt C, Bcl2, Bax, caspases 3) were determined. In 
      order to elucidate the underlying mechanism, the expression of HIF-1alpha and BNIP3 
      were assessed. Our results showed that treatment of PNS rescued the cell 
      viability of CDDP-injured HK-2 or HIF-1alpha-knockdown HK-2 cells, and increased the 
      expression levels of ATP and MMP in HK-2 or HIF-1alpha-knockdown HK-2 cells which 
      were reduced by CDDP. Moreover, PNS treatment decreased the CDDP or CDDP plus 
      HIF-1alpha-knockdown-induced elevation of apoptosis and apoptosis-associated protein 
      expressions. These findings demonstrate that PNS reduces CAKI through increasing 
      HIF-1alpha to inhibit mitochondrial apoptosis pathway. Hence, we suggest PNS as a 
      protective and therapeutic new drug for CDDP treatment of cancers, which might 
      have significant meaning of further research and application potential.
CI  - Copyright (c) 2021 The Authors. Published by Elsevier Masson SAS.. All rights 
      reserved.
FAU - Li, Qingqing
AU  - Li Q
AD  - Postgraduate, Pharmacy Department, the first affiliated hospital of Guangxi 
      Medical University, Nanning, China.
FAU - Zhang, Yansong
AU  - Zhang Y
AD  - Postgraduate, Pharmacy Department, the first affiliated hospital of Guangxi 
      Medical University, Nanning, China.
FAU - Yang, Yufang
AU  - Yang Y
AD  - Pharmacy Department, the first affiliated hospital of Guangxi Medical University, 
      Nanning, China. Electronic address: yufangyang@gxmu.edu.cn.
FAU - Huang, Songqing
AU  - Huang S
AD  - Postgraduate, Pharmacy Department, the first affiliated hospital of Guangxi 
      Medical University, Nanning, China.
FAU - Zou, Xiaoqin
AU  - Zou X
AD  - Pharmacy Department, the first affiliated hospital of Guangxi Medical University, 
      Nanning, China.
FAU - Wei, Congying
AU  - Wei C
AD  - Postgraduate, Pharmacy Department, the first affiliated hospital of Guangxi 
      Medical University, Nanning, China.
FAU - Liang, Taolin
AU  - Liang T
AD  - Postgraduate, Pharmacy Department, the first affiliated hospital of Guangxi 
      Medical University, Nanning, China.
FAU - Zhong, Xiaobin
AU  - Zhong X
AD  - Regenerative Medicine Research Center of Guangxi Medical University, Nanning, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20210809
PL  - France
TA  - Biomed Pharmacother
JT  - Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
JID - 8213295
RN  - 0 (Antineoplastic Agents)
RN  - 0 (BNIP3 protein, human)
RN  - 0 (HIF1A protein, human)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (Membrane Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Saponins)
RN  - Q20Q21Q62J (Cisplatin)
SB  - IM
MH  - Acute Kidney Injury/chemically induced/*prevention & control
MH  - Animals
MH  - Antineoplastic Agents/toxicity
MH  - Apoptosis/drug effects
MH  - Cell Line
MH  - Cell Survival/drug effects
MH  - Cisplatin/*toxicity
MH  - Epithelial Cells/drug effects/pathology
MH  - Gene Knockdown Techniques
MH  - Humans
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/genetics
MH  - Kidney Tubules, Proximal/cytology/drug effects/pathology
MH  - Membrane Proteins
MH  - Mitochondria/drug effects/pathology
MH  - Panax notoginseng/*chemistry
MH  - Proto-Oncogene Proteins
MH  - Rats
MH  - Saponins/isolation & purification/*pharmacology
OTO - NOTNLM
OT  - Cisplatin-induced acute kidney injury
OT  - HIF-1alpha-siRNA
OT  - Mitochondrial apoptosis pathway
OT  - Panax notoginseng saponins
EDAT- 2021/08/14 06:00
MHDA- 2022/01/07 06:00
CRDT- 2021/08/13 06:10
PHST- 2021/06/14 00:00 [received]
PHST- 2021/07/22 00:00 [revised]
PHST- 2021/07/22 00:00 [accepted]
PHST- 2021/08/14 06:00 [pubmed]
PHST- 2022/01/07 06:00 [medline]
PHST- 2021/08/13 06:10 [entrez]
AID - S0753-3322(21)00747-2 [pii]
AID - 10.1016/j.biopha.2021.111965 [doi]
PST - ppublish
SO  - Biomed Pharmacother. 2021 Oct;142:111965. doi: 10.1016/j.biopha.2021.111965. Epub 
      2021 Aug 9.