PMID- 34385329
OWN - NLM
STAT- MEDLINE
DCOM- 20211213
LR  - 20220716
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 118
IP  - 33
DP  - 2021 Aug 17
TI  - Recombination-independent recognition of DNA homology for meiotic silencing in 
      Neurospora crassa.
LID - 10.1073/pnas.2108664118 [doi]
LID - e2108664118
AB  - The pairing of homologous chromosomes represents a critical step of meiosis in 
      nearly all sexually reproducing species. In many organisms, pairing involves 
      chromosomes that remain apparently intact. The mechanistic nature of homology 
      recognition at the basis of such pairing is unknown. Using "meiotic silencing by 
      unpaired DNA" (MSUD) as a model process, we demonstrate the existence of a 
      cardinally different approach to DNA homology recognition in meiosis. The main 
      advantage of MSUD over other experimental systems lies in its ability to identify 
      any relatively short DNA fragment lacking a homologous allelic partner. Here, we 
      show that MSUD does not rely on the canonical mechanism of meiotic recombination, 
      yet it is promoted by REC8, a conserved component of the meiotic cohesion 
      complex. We also show that certain patterns of interspersed homology are 
      recognized as pairable during MSUD. Such patterns need to be colinear and must 
      contain short tracts of sequence identity spaced apart at 21 or 22 base pairs. By 
      using these periodicity values as a guiding parameter in all-atom molecular 
      modeling, we discover that homologous DNA molecules can pair by forming 
      quadruplex-based contacts with an interval of 2.5 helical turns. This process 
      requires right-handed plectonemic coiling and additional conformational changes 
      in the intervening double-helical segments. Our results 1) reconcile genetic and 
      biophysical evidence for the existence of direct homologous double-stranded DNA 
      (dsDNA)-dsDNA pairing, 2) identify a role for this process in initiating RNA 
      interference, and 3) suggest that chromosomes can be cross-matched by a precise 
      mechanism that operates on intact dsDNA molecules.
FAU - Rhoades, Nicholas
AU  - Rhoades N
AD  - School of Biological Sciences, Illinois State University, Normal, IL 61761.
FAU - Nguyen, Tinh-Suong
AU  - Nguyen TS
AUID- ORCID: 0000-0002-2115-4370
AD  - Group Fungal Epigenomics, Department of Mycology, Institut Pasteur, Paris 75015, 
      France.
FAU - Witz, Guillaume
AU  - Witz G
AD  - Microscopy Imaging Center; Science IT Support, Mathematical Institute, University 
      of Bern, Bern 3005, Switzerland.
FAU - Cecere, Germano
AU  - Cecere G
AD  - Mechanisms of Epigenetic Inheritance, Department of Developmental and Stem Cell 
      Biology, UMR 3738, CNRS, Institut Pasteur, Paris 75015, France.
FAU - Hammond, Thomas
AU  - Hammond T
AUID- ORCID: 0000-0002-0812-4759
AD  - School of Biological Sciences, Illinois State University, Normal, IL 61761.
FAU - Mazur, Alexey K
AU  - Mazur AK
AD  - Group Fungal Epigenomics, Department of Mycology, Institut Pasteur, Paris 75015, 
      France.
AD  - Laboratoire de Biochimie Theorique, UPR 9080 CNRS, Universite de Paris, Paris 
      75005, France.
FAU - Gladyshev, Eugene
AU  - Gladyshev E
AUID- ORCID: 0000-0002-4074-4966
AD  - Group Fungal Epigenomics, Department of Mycology, Institut Pasteur, Paris 75015, 
      France; eugene.gladyshev@gmail.com.
LA  - eng
GR  - R15 HD076309/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (DNA, Fungal)
SB  - IM
CIN - Proc Natl Acad Sci U S A. 2021 Sep 28;118(39):. PMID: 34544880
MH  - Chromosomes, Fungal/genetics/*physiology
MH  - DNA, Fungal/*genetics
MH  - Gene Expression Regulation, Fungal/*physiology
MH  - Meiosis/genetics/*physiology
MH  - Neurospora crassa/*physiology
MH  - Recombination, Genetic/genetics/*physiology
PMC - PMC8379962
OTO - NOTNLM
OT  - DNA
OT  - MSUD
OT  - Neurospora
OT  - homology recognition
OT  - meiosis
COIS- The authors declare no competing interest.
EDAT- 2021/08/14 06:00
MHDA- 2021/12/15 06:00
PMCR- 2022/02/12
CRDT- 2021/08/13 06:13
PHST- 2021/08/13 06:13 [entrez]
PHST- 2021/08/14 06:00 [pubmed]
PHST- 2021/12/15 06:00 [medline]
PHST- 2022/02/12 00:00 [pmc-release]
AID - 2108664118 [pii]
AID - 202108664 [pii]
AID - 10.1073/pnas.2108664118 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2021 Aug 17;118(33):e2108664118. doi: 
      10.1073/pnas.2108664118.