PMID- 34386989
OWN - NLM
STAT- MEDLINE
DCOM- 20220119
LR  - 20220119
IS  - 1365-2842 (Electronic)
IS  - 0305-182X (Linking)
VI  - 49
IP  - 2
DP  - 2022 Feb
TI  - Stem cells from human exfoliated deciduous teeth relieve pain via downregulation 
      of c-Jun in a rat model of trigeminal neuralgia.
PG  - 219-227
LID - 10.1111/joor.13243 [doi]
AB  - BACKGROUND: Stem cells from human exfoliated deciduous teeth (SHED) have 
      excellent immunomodulatory and neuroprotective abilities. It is possible that 
      systemic SHED transplantation could ameliorate trigeminal neuralgia. The 
      phosphorylation of c-Jun contributes to the development of hyperalgesia and 
      allodynia. OBJECTIVE: The present study aimed to evaluate whether systemic SHED 
      transplantation could lead to analgesic effects by regulating peripheral c-Jun in 
      the trigeminal ganglia (TG) in a rat model of trigeminal neuralgia. METHODS: 
      Chronic constriction injury of the infraorbital nerve (CCI-ION) was performed to 
      establish a rat pain model. SHED were obtained from discarded exfoliated 
      deciduous teeth from children and transplanted by a single infusion through the 
      tail vein. SHED were labelled with the PKH26 red fluorescent cell linker mini kit 
      for tract distribution. The mechanical threshold was determined using von Frey 
      filaments. The mRNA levels of c-Jun in the ipsilateral TG were quantified. The 
      phosphorylation of c-Jun in the ipsilateral TG was assessed by 
      immunohistochemistry and Western blotting. RESULTS: PKH26-labelled SHED were 
      distributed to both sides of TG, lung, liver and spleen. Systemic SHED 
      transplantation significantly elevated the mechanical thresholds in CCI-ION rats 
      and blocked the upregulation of c-Jun mRNA levels in the TG caused by nerve 
      ligation. The activation of c-Jun in the TG was blocked by SHED transplantation. 
      CONCLUSIONS: These findings demonstrate that systemic SHED administration reverts 
      trigeminal neuralgia via downregulation of c-Jun in the TG.
CI  - (c) 2021 John Wiley & Sons Ltd.
FAU - Bai, Xiaofeng
AU  - Bai X
AD  - Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, China 
      Medical University, Shenyang, China.
AD  - Liaoning Provincial Key Laboratory of Oral Diseases, Shenyang, China.
FAU - Xiao, Ke
AU  - Xiao K
AD  - Liaoning Provincial Key Laboratory of Oral Diseases, Shenyang, China.
AD  - Painless Dental Treatment Center, Hospital of Stomatology, China Medical 
      University, Shenyang, China.
FAU - Yang, Zhijie
AU  - Yang Z
AD  - Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, China 
      Medical University, Shenyang, China.
AD  - Liaoning Provincial Key Laboratory of Oral Diseases, Shenyang, China.
FAU - Zhang, Ziqi
AU  - Zhang Z
AD  - Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, China 
      Medical University, Shenyang, China.
AD  - Liaoning Provincial Key Laboratory of Oral Diseases, Shenyang, China.
FAU - Li, Jing
AU  - Li J
AD  - Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, China 
      Medical University, Shenyang, China.
AD  - Liaoning Provincial Key Laboratory of Oral Diseases, Shenyang, China.
FAU - Yan, Ziyi
AU  - Yan Z
AD  - Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, China 
      Medical University, Shenyang, China.
AD  - Liaoning Provincial Key Laboratory of Oral Diseases, Shenyang, China.
FAU - Cao, Keda
AU  - Cao K
AD  - Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, China 
      Medical University, Shenyang, China.
AD  - Liaoning Provincial Key Laboratory of Oral Diseases, Shenyang, China.
FAU - Zhang, Weiqian
AU  - Zhang W
AD  - Hospital of Stomatology, China Medical University, Shenyang, China.
FAU - Zhang, Xia
AU  - Zhang X
AUID- ORCID: 0000-0002-7996-4843
AD  - Liaoning Provincial Key Laboratory of Oral Diseases, Shenyang, China.
AD  - Painless Dental Treatment Center, Hospital of Stomatology, China Medical 
      University, Shenyang, China.
LA  - eng
GR  - 81400530/National Natural Science Foundation of China/
GR  - 20180530097/Natural Science Foundation of Liaoning Province/
GR  - 201602811/Natural Science Foundation of Liaoning Province/
PT  - Journal Article
DEP - 20210817
PL  - England
TA  - J Oral Rehabil
JT  - Journal of oral rehabilitation
JID - 0433604
SB  - IM
MH  - Animals
MH  - Down-Regulation
MH  - Humans
MH  - Hyperalgesia
MH  - Pain
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Stem Cells
MH  - Tooth, Deciduous
MH  - *Trigeminal Neuralgia
OTO - NOTNLM
OT  - c-Jun
OT  - infraorbital nerve
OT  - pain relief
OT  - stem cells from human exfoliated deciduous teeth
OT  - trigeminal ganglia
OT  - trigeminal neuralgia
EDAT- 2021/08/14 06:00
MHDA- 2022/01/20 06:00
CRDT- 2021/08/13 07:23
PHST- 2021/07/01 00:00 [received]
PHST- 2021/08/10 00:00 [accepted]
PHST- 2021/08/14 06:00 [pubmed]
PHST- 2022/01/20 06:00 [medline]
PHST- 2021/08/13 07:23 [entrez]
AID - 10.1111/joor.13243 [doi]
PST - ppublish
SO  - J Oral Rehabil. 2022 Feb;49(2):219-227. doi: 10.1111/joor.13243. Epub 2021 Aug 
      17.