PMID- 34391409
OWN - NLM
STAT- MEDLINE
DCOM- 20220110
LR  - 20220110
IS  - 1472-6823 (Electronic)
IS  - 1472-6823 (Linking)
VI  - 21
IP  - 1
DP  - 2021 Aug 14
TI  - Areca catechu-(Betel-nut)-induced whole transcriptome changes in a human monocyte 
      cell line that may have relevance to diabetes and obesity; a pilot study.
PG  - 165
LID - 10.1186/s12902-021-00827-1 [doi]
LID - 165
AB  - BACKGROUND: Betel-nut consumption is the fourth most common addictive habit 
      globally and there is good evidence linking the habit to obesity, type 2 diabetes 
      (T2D) and the metabolic syndrome. The aim of our pilot study was to identify gene 
      expression relevant to obesity, T2D and the metabolic syndrome using a 
      genome-wide transcriptomic approach in a human monocyte cell line incubated with 
      arecoline and its nitrosated products. RESULTS: The THP1 monocyte cell line was 
      incubated separately with arecoline and 3-methylnitrosaminopropionaldehyde (MNPA) 
      in triplicate for 24 h and pooled cDNA indexed paired-end libraries were 
      sequenced (Illumina NextSeq 500). After incubation with arecoline and MNPA, 15 
      and 39 genes respectively had significant changes in their expression (q < 0.05, 
      log fold change 1.5). Eighteen of those genes have reported associations with T2D 
      and obesity in humans; of these genes there was most marked evidence for CLEC10A, 
      MAPK8IP1, NEGR1, NQ01 and INHBE genes. CONCLUSIONS: Our preliminary studies have 
      identified a large number of genes relevant to obesity, T2D and metabolic 
      syndrome whose expression was changed significantly in human TPH1 cells following 
      incubation with betel-nut derived arecoline or with MNPA. These findings require 
      validation by further cell-based work and investigation amongst betel-chewing 
      communities.
CI  - (c) 2021. The Author(s).
FAU - Cardosa, Shirleny R
AU  - Cardosa SR
AD  - Centre for Genomics and Child Health, Blizard Institute, Barts and the London 
      School of Medicine and Dentistry, Queen Mary University of London, London, UK.
FAU - Ogunkolade, B William
AU  - Ogunkolade BW
AD  - Centre for Genomics and Child Health, Blizard Institute, Barts and the London 
      School of Medicine and Dentistry, Queen Mary University of London, London, UK.
FAU - Lowe, Rob
AU  - Lowe R
AD  - Centre for Genomics and Child Health, Blizard Institute, Barts and the London 
      School of Medicine and Dentistry, Queen Mary University of London, London, UK.
FAU - Savage, Emanuel
AU  - Savage E
AD  - Barts and The London Genome Centre, Blizard Institute, Queen Mary University of 
      London, London, UK.
FAU - Mein, Charles A
AU  - Mein CA
AD  - Barts and The London Genome Centre, Blizard Institute, Queen Mary University of 
      London, London, UK.
FAU - Boucher, Barbara J
AU  - Boucher BJ
AD  - Centre for Genomics and Child Health, Blizard Institute, Barts and the London 
      School of Medicine and Dentistry, Queen Mary University of London, London, UK.
FAU - Hitman, Graham A
AU  - Hitman GA
AD  - Centre for Genomics and Child Health, Blizard Institute, Barts and the London 
      School of Medicine and Dentistry, Queen Mary University of London, London, UK. 
      g.a.hitman@qmul.ac.uk.
LA  - eng
GR  - MRC0183/Barts and the London Charity/
PT  - Journal Article
DEP - 20210814
PL  - England
TA  - BMC Endocr Disord
JT  - BMC endocrine disorders
JID - 101088676
RN  - 0 (Biomarkers)
RN  - 4ALN5933BH (Arecoline)
SB  - IM
MH  - Areca/*chemistry
MH  - Arecoline/*pharmacology
MH  - Biomarkers/analysis/metabolism
MH  - Diabetes Mellitus, Type 2/*genetics
MH  - Follow-Up Studies
MH  - Gene Expression Regulation/*drug effects
MH  - Humans
MH  - Metabolic Syndrome/*genetics
MH  - Monocytes/drug effects/*metabolism/pathology
MH  - Obesity/*genetics
MH  - Pilot Projects
MH  - Prognosis
MH  - Transcriptome/*drug effects
PMC - PMC8364090
OTO - NOTNLM
OT  - Betel-nut
OT  - Obesity
OT  - RNA-sequencing
OT  - Transcriptomics
OT  - Type 2 diabetes
COIS- None.
EDAT- 2021/08/16 06:00
MHDA- 2022/01/11 06:00
PMCR- 2021/08/14
CRDT- 2021/08/15 20:22
PHST- 2021/01/07 00:00 [received]
PHST- 2021/07/26 00:00 [accepted]
PHST- 2021/08/15 20:22 [entrez]
PHST- 2021/08/16 06:00 [pubmed]
PHST- 2022/01/11 06:00 [medline]
PHST- 2021/08/14 00:00 [pmc-release]
AID - 10.1186/s12902-021-00827-1 [pii]
AID - 827 [pii]
AID - 10.1186/s12902-021-00827-1 [doi]
PST - epublish
SO  - BMC Endocr Disord. 2021 Aug 14;21(1):165. doi: 10.1186/s12902-021-00827-1.