PMID- 34393240
OWN - NLM
STAT- MEDLINE
DCOM- 20210817
LR  - 20220716
IS  - 1671-167X (Print)
IS  - 1671-167X (Linking)
VI  - 53
IP  - 4
DP  - 2021 Aug 18
TI  - [Effect of topical injection of cyclosporine A on saliva secretion and 
      inflammation in the submandibular gland of non-obese diabetic mice].
PG  - 750-757
AB  - OBJECTIVE: To investigate the effects of topical administration of cyclosporine A 
      (CsA) on salivary secretion and inflammation of the submandibular glands in 
      non-obese diabetic (NOD) mice. METHODS: Female NOD mice, 21 aged 14 weeks and 18 
      aged 21 weeks were selected and randomly divided into low-dose group, high-dose 
      group and control group on average. CsA was injected into submandibular glands. 
      One week later the saliva stimulated by pilocarpine was collected and measured. 
      The submandibular glands were collected to make paraffin sections. The lymphocyte 
      infiltration in submandi-bular gland was observed by microscope after 
      hematoxylin-eosin (HE) staining. The number of lymphocyte infiltration foci was 
      counted to calculate the focus sore and the ratio of lymphocyte infiltration area 
      to total gland area was figured up by Leica image analysis system. The 
      expressions of inflammatory cytokines tumor necrosis factor-alpha (TNF-alpha), 
      interferon-gamma (IFN-gamma), interleukin-4 (IL-4), IL-13, IL-17F, IL22 and IL-23a in the 
      submandibular glands of the NOD mice were detected by quantitative real-time 
      polymerase chain reaction (qRT-PCR). Cell apoptosis in the submandibular gland 
      was detected by terminal deoxynucleotidyl transferase-mediated dUTP nick end 
      labeling (TUNEL). The levels of serum creatinine (Scr), blood urea nitrogen 
      (BUN), uric acid (UA), alanine aminotransferase (ALT), aspertate aminotransferase 
      (AST), alkaline phosphatase (ALP), albumin (ALB) and gamma-glutamyl transferase (GGT) 
      were measured by automatic biochemical analyzer to evaluate liver and kidney 
      functions. RESULTS: After topical injection of CsA in the submandibular gland, 
      the stimulated salivary flow rate of the 14- and 21-week-old NOD mice 
      significantly increased compared with the control group (P < 0.01 or P < 0.05), 
      and the number and area of lymphocyte infiltration foci in the 14-week-old NOD 
      mice low-dose group significantly decreased compared with the control group (P < 
      0.01). Low and high dose of CsA had similar effects on reducing inflammation and 
      improving salivary secretion. The overall level of inflammatory cytokines in the 
      submandibular gland did not decrease significantly. The number of cell apoptosis 
      of submandibular gland in the NOD mice treated with CsA decreased compared with 
      the control group, but there was no statistically significant difference. Topical 
      injection of CsA had no adverse effect on liver and kidney function in the NOD 
      mice. CONCLUSION: Topical injection of CsA can reduce lymphocyte infiltration in 
      submandibular gland of NOD mice and improve salivary secretion.
FAU - Zhu, Y Y
AU  - Zhu YY
AD  - Department of Oral and Maxilloficial Surgery, Peking University School and 
      Hospital of Stomatology & National Center of Stomatology & National Clinical 
      Research Center for Oral Diseases & National Engineering Laboratory for Digital 
      and Material Technology of Stomatology & Beijing Key Laboratory of Digital 
      Stomatology, Beijing 100081, China.
FAU - Min, S N
AU  - Min SN
AD  - Department of Oral and Maxilloficial Surgery, Peking University School and 
      Hospital of Stomatology & National Center of Stomatology & National Clinical 
      Research Center for Oral Diseases & National Engineering Laboratory for Digital 
      and Material Technology of Stomatology & Beijing Key Laboratory of Digital 
      Stomatology, Beijing 100081, China.
FAU - Yu, G Y
AU  - Yu GY
AD  - Department of Oral and Maxilloficial Surgery, Peking University School and 
      Hospital of Stomatology & National Center of Stomatology & National Clinical 
      Research Center for Oral Diseases & National Engineering Laboratory for Digital 
      and Material Technology of Stomatology & Beijing Key Laboratory of Digital 
      Stomatology, Beijing 100081, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Beijing Da Xue Xue Bao Yi Xue Ban
JT  - Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health 
      sciences
JID - 101125284
RN  - 83HN0GTJ6D (Cyclosporine)
SB  - IM
MH  - Animals
MH  - Cyclosporine
MH  - *Diabetes Mellitus, Experimental/drug therapy
MH  - Disease Models, Animal
MH  - Female
MH  - Inflammation
MH  - Mice
MH  - Mice, Inbred NOD
MH  - Saliva
MH  - *Sjogren's Syndrome
MH  - Submandibular Gland
PMC - PMC8365053
OTO - NOTNLM
OT  - Sjogren's syndrome
OT  - cyclosporine A
OT  - non-obese diabetic mice
OT  - salivary gland
OT  - submandibular gland
EDAT- 2021/08/17 06:00
MHDA- 2021/08/18 06:00
PMCR- 2021/08/18
CRDT- 2021/08/16 05:38
PHST- 2021/08/16 05:38 [entrez]
PHST- 2021/08/17 06:00 [pubmed]
PHST- 2021/08/18 06:00 [medline]
PHST- 2021/08/18 00:00 [pmc-release]
AID - bjdxxbyxb-53-4-750 [pii]
AID - 10.19723/j.issn.1671-167X.2021.04.022 [doi]
PST - ppublish
SO  - Beijing Da Xue Xue Bao Yi Xue Ban. 2021 Aug 18;53(4):750-757. doi: 
      10.19723/j.issn.1671-167X.2021.04.022.