PMID- 34394055 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20210817 IS - 1664-302X (Print) IS - 1664-302X (Electronic) IS - 1664-302X (Linking) VI - 12 DP - 2021 TI - Effect of Sublethal Prenatal Endotoxaemia on Murine Placental Transport Systems and Lipid Homeostasis. PG - 706499 LID - 10.3389/fmicb.2021.706499 [doi] LID - 706499 AB - Infection alters the expression of transporters that mediate the placental exchange of xenobiotics, lipids and cytokines. We hypothesized that lipopolysaccharide (LPS) modifies the expression of placental transport systems and lipid homeostasis. LPS (150 mug/kg; i.p.) treatments were administered for 4 h or 24 h, animals were euthanized at gestational days (GD) 15.5 or 18.5, and maternal blood, fetuses and placentae were collected. Increased rates of fetal demise were observed at GD15.5 following LPS treatment, whereas at GD18.5, high rates of early labour occurred and were associated with distinct proinflammatory responses. Lipopolysaccharide did not alter ATP-binding cassette (ABC) transporter mRNA expression but decreased fatty acid binding protein associated with plasma membrane (Fabppm) at GD15.5 (LPS-4 h) and increased fatty acid translocase (Fat/Cd36) mRNA at GD18.5 (LPS-4 h). At the protein level, breast cancer-related protein (Bcrp) and ABC sub-family G member 1 (Abcg1) levels were decreased in the placental labyrinth zone (Lz) at GD15.5, whereas P-glycoprotein (P-gp) and Bcrp Lz-immunostaining was decreased at GD18.5. In the placental junctional zone (Jz), P-gp, Bcrp and Abcg1 levels were higher at GD18.5. Specific maternal plasma and placental changes in triacylglycerol, free fatty acid, cholesterol, cholesterol ester and monoacylglycerol levels were detected in a gestational age-dependent manner. In conclusion, LPS-increased risk of fetal death and early labour were associated with altered placental ABC and lipid transporter expression and deranged maternal plasma and placental lipid homeostasis. These changes may potentially modify fetal xenobiotic exposure and placental lipid exchange in cases of bacterial infection. CI - Copyright (c) 2021 Reginatto, Fontes, Monteiro, Silva, Andrade, Gomes, Imperio, Bloise, Kluck, Atella, Matthews, Bloise and Ortiga-Carvalho. FAU - Reginatto, Mila W AU - Reginatto MW AD - Laboratory of Translational Endocrinology, Institute of Biophysics Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil. FAU - Fontes, Klaus Novaes AU - Fontes KN AD - Laboratory of Translational Endocrinology, Institute of Biophysics Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil. FAU - Monteiro, Victoria R S AU - Monteiro VRS AD - Laboratory of Translational Endocrinology, Institute of Biophysics Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil. FAU - Silva, Natalia L AU - Silva NL AD - Laboratory of Translational Endocrinology, Institute of Biophysics Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil. FAU - Andrade, Cherley Borba Vieira AU - Andrade CBV AD - Laboratory of Translational Endocrinology, Institute of Biophysics Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil. FAU - Gomes, Hanailly Ribeiro AU - Gomes HR AD - Laboratory of Translational Endocrinology, Institute of Biophysics Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil. FAU - Imperio, Guinever E AU - Imperio GE AD - Laboratory of Translational Endocrinology, Institute of Biophysics Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil. AD - Department of Physiology, Faculty of Medicine, University of Toronto, Toronto, ON, Canada. AD - Lunenfeld-Tanenbaum Research Institute of Medical, Sinai Health System, Toronto, ON, Canada. FAU - Bloise, Flavia Fonseca AU - Bloise FF AD - Laboratory of Translational Endocrinology, Institute of Biophysics Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil. FAU - Kluck, George Eduardo Gabriel AU - Kluck GEG AD - Laboratory of Lipids and Lipoproteins Biochemistry, Institute of Medical Biochemistry Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil. FAU - Atella, Georgia Correa AU - Atella GC AD - Laboratory of Lipids and Lipoproteins Biochemistry, Institute of Medical Biochemistry Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil. FAU - Matthews, Stephen G AU - Matthews SG AD - Department of Physiology, Faculty of Medicine, University of Toronto, Toronto, ON, Canada. AD - Lunenfeld-Tanenbaum Research Institute of Medical, Sinai Health System, Toronto, ON, Canada. AD - Department of Obstetrics and Gynecology, Faculty of Medicine, University of Toronto, Toronto, ON, Canada. AD - Department of Medicine, Faculty of Medicine, University of Toronto, Toronto, ON, Canada. FAU - Bloise, Enrrico AU - Bloise E AD - Department of Morphology, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil. FAU - Ortiga-Carvalho, Tania M AU - Ortiga-Carvalho TM AD - Laboratory of Translational Endocrinology, Institute of Biophysics Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil. LA - eng PT - Journal Article DEP - 20210730 PL - Switzerland TA - Front Microbiol JT - Frontiers in microbiology JID - 101548977 PMC - PMC8363225 OTO - NOTNLM OT - ABC sub-family G member 1 OT - ATP binding cassette transporters OT - FAtty acid translocase OT - P-glycoprotein OT - breast cancer resistance protein OT - cholesterol OT - fatty acid binding protein associated with plasma membrane OT - lipopolysaccharide EDAT- 2021/08/17 06:00 MHDA- 2021/08/17 06:01 PMCR- 2021/07/30 CRDT- 2021/08/16 05:48 PHST- 2021/05/07 00:00 [received] PHST- 2021/06/25 00:00 [accepted] PHST- 2021/08/16 05:48 [entrez] PHST- 2021/08/17 06:00 [pubmed] PHST- 2021/08/17 06:01 [medline] PHST- 2021/07/30 00:00 [pmc-release] AID - 10.3389/fmicb.2021.706499 [doi] PST - epublish SO - Front Microbiol. 2021 Jul 30;12:706499. doi: 10.3389/fmicb.2021.706499. eCollection 2021.