PMID- 34394357
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210817
IS  - 1687-966X (Print)
IS  - 1687-9678 (Electronic)
VI  - 2021
DP  - 2021
TI  - CGF Membrane Promotes Periodontal Tissue Regeneration Mediated by hUCMSCs through 
      Upregulating TAZ and Osteogenic Differentiation Genes.
PG  - 6644366
LID - 10.1155/2021/6644366 [doi]
LID - 6644366
AB  - Concentrated growth factor (CGF) membranes are widely used in basic and clinical 
      research of soft and hard tissue regeneration, but its effect on periodontal 
      tissue regeneration is less studied. This study explored the role of CGF 
      membranes in periodontal tissue regeneration mediated by human umbilical cord 
      mesenchymal stem cells (hUCMSCs). HUCMSCs and human periodontal ligament 
      fibroblasts (HPLFs) were extracted and identified by microscope and flow 
      cytometry. The effects of the extracted CGF membrane on cell viability, 
      osteogenic differentiation ability, osteopontin (OPN) expression, alkaline 
      phosphatase (ALP) content, and osteogenic differentiation-related genes 
      (Runt-related transcription factor 2 (RUNX2); osteocalcin (OCN); ALP), Tafazzin 
      (TAZ) expression, and nuclear transfer were examined by MTT assay, alizarin red 
      staining, immunofluorescence, enzyme-linked immunosorbent assay (ELISA), 
      quantitative real-time polymerase chain reaction (qRT-PCR), and Western blot. 
      Rescue experiments were performed to examine the effects of TAZ transfection and 
      cell coculture. In the identified hUCMSCs (positive expressions of CD29, CD44, 
      CD146, and CD105), overexpressed TAZ (pc-TAZ) enhanced the promotive effect of 
      CGF membrane on cell viability, cell cycle, mineralization, ALP content and 
      expressions of OPN, TAZ and osteogenic differentiation-related genes, and nuclear 
      transfer. However, silencing TAZ showed opposite effects. The coculture of 
      hUCMSCs and HPLFs further promoted the basic biological functions of HPLFs by 
      upregulating osteogenic differentiation-related genes and COL-1 but downregulated 
      MMP1 expression. Pc-TAZ could enhance the effect of CGF membrane on promoting 
      periodontal tissue regeneration. CGF membrane promoted periodontal tissue 
      regeneration through upregulating TAZ and osteogenic differentiation-related 
      genes.
CI  - Copyright (c) 2021 Wenjing Li et al.
FAU - Li, Wenjing
AU  - Li W
AD  - Department of Oral Pathology, School and Hospital of Stomatology, Hebei Medical 
      University, The Key Laboratory of Stomatology, 419 East Zhongshan Road, 
      Shijiazhuang Hebei 050017, China.
AD  - Department of Oral Medicine, The Second Hospital of Hebei Medical University, 
      China.
FAU - Wang, Feifei
AU  - Wang F
AD  - Department of Stomatology, The Third Hospital of Hebei Medical University, China.
FAU - Dong, Fusheng
AU  - Dong F
AD  - Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, 
      Hebei Medical University, China.
FAU - Zhang, Zhiyong
AU  - Zhang Z
AD  - Department of Oral Medicine, The Second Hospital of Hebei Medical University, 
      China.
FAU - Song, Peng
AU  - Song P
AD  - Department of Oral Pathology, School and Hospital of Stomatology, Hebei Medical 
      University, The Key Laboratory of Stomatology, 419 East Zhongshan Road, 
      Shijiazhuang Hebei 050017, China.
FAU - Chen, Huizhen
AU  - Chen H
AD  - Department of Oral Medicine, The Second Hospital of Hebei Medical University, 
      China.
FAU - Wang, Jie
AU  - Wang J
AUID- ORCID: 0000-0002-5461-744X
AD  - Department of Oral Pathology, School and Hospital of Stomatology, Hebei Medical 
      University, The Key Laboratory of Stomatology, 419 East Zhongshan Road, 
      Shijiazhuang Hebei 050017, China.
LA  - eng
PT  - Journal Article
DEP - 20210804
PL  - United States
TA  - Stem Cells Int
JT  - Stem cells international
JID - 101535822
PMC - PMC8360720
COIS- The authors declare no conflicts of interest.
EDAT- 2021/08/17 06:00
MHDA- 2021/08/17 06:01
PMCR- 2021/08/04
CRDT- 2021/08/16 05:50
PHST- 2020/12/03 00:00 [received]
PHST- 2021/06/22 00:00 [revised]
PHST- 2021/07/19 00:00 [accepted]
PHST- 2021/08/16 05:50 [entrez]
PHST- 2021/08/17 06:00 [pubmed]
PHST- 2021/08/17 06:01 [medline]
PHST- 2021/08/04 00:00 [pmc-release]
AID - 10.1155/2021/6644366 [doi]
PST - epublish
SO  - Stem Cells Int. 2021 Aug 4;2021:6644366. doi: 10.1155/2021/6644366. eCollection 
      2021.