PMID- 34395766
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220210
IS  - 2331-8325 (Electronic)
IS  - 2331-8325 (Linking)
VI  - 8
IP  - 15
DP  - 2018 Aug 5
TI  - Direct Visualization of the Multicopy Chromosomes in Cyanobacterium Synechococcus 
      elongatus PCC 7942.
PG  - e2958
LID - 10.21769/BioProtoc.2958 [doi]
LID - e2958
AB  - Cyanobacteria are prokaryotic organisms that carry out oxygenic photosynthesis. 
      The fresh water cyanobacterium Synechococcus elongatus PCC 7942 is a model 
      organism for the study of photosynthesis and gene regulation, and for 
      biotechnological applications. Besides several freshwater cyanobacteria, S. 
      elongatus 7942 also contains multiple chromosomal copies per cell at all stages 
      of its cell cycle. Here, we describe a method for the direct visualization of 
      multicopy chromosomes in S. elongatus 7942 by fluorescence in situ hybridization 
      (FISH).
CI  - Copyright (c) 2018 The Authors; exclusive licensee Bio-protocol LLC.
FAU - Ohbayashi, Ryudo
AU  - Ohbayashi R
AD  - Department of Cell Genetics, National Institute of Genetics, Shizuoka, Japan.
FAU - Yoshikawa, Hirofumi
AU  - Yoshikawa H
AD  - Department of Bioscience, Tokyo University of Agriculture, Setagaya-ku, Tokyo, 
      Japan.
FAU - Watanabe, Satoru
AU  - Watanabe S
AD  - Department of Bioscience, Tokyo University of Agriculture, Setagaya-ku, Tokyo, 
      Japan.
LA  - eng
PT  - Journal Article
DEP - 20180805
PL  - United States
TA  - Bio Protoc
JT  - Bio-protocol
JID - 101635102
PMC - PMC8328676
OTO - NOTNLM
OT  - Chromosome distribution
OT  - Cyanobacteria
OT  - In situ hybridization
OT  - Polyploidy
OT  - Replication origin
EDAT- 2018/08/05 00:00
MHDA- 2018/08/05 00:01
PMCR- 2019/08/05
CRDT- 2021/08/16 06:04
PHST- 2018/05/11 00:00 [received]
PHST- 2018/07/16 00:00 [revised]
PHST- 2018/07/31 00:00 [accepted]
PHST- 2021/08/16 06:04 [entrez]
PHST- 2018/08/05 00:00 [pubmed]
PHST- 2018/08/05 00:01 [medline]
PHST- 2019/08/05 00:00 [pmc-release]
AID - e2958 [pii]
AID - 2958 [pii]
AID - 10.21769/BioProtoc.2958 [doi]
PST - epublish
SO  - Bio Protoc. 2018 Aug 5;8(15):e2958. doi: 10.21769/BioProtoc.2958. eCollection 
      2018 Aug 5.