PMID- 34397058
OWN - NLM
STAT- MEDLINE
DCOM- 20220125
LR  - 20220125
IS  - 2042-650X (Electronic)
IS  - 2042-6496 (Linking)
VI  - 12
IP  - 19
DP  - 2021 Oct 4
TI  - DAF-16 acts as the "hub" of astaxanthin's anti-aging mechanism to improve 
      aging-related physiological functions in Caenorhabditis elegans.
PG  - 9098-9110
LID - 10.1039/d1fo01069g [doi]
AB  - Astaxanthin (AX) is a xanthophyll carotenoid that can effectively inhibit the 
      production of peroxides and thereby protect the body from oxidative damage. In 
      recent years, AX had been shown to have anti-aging properties, both in vivo and 
      in vitro. However, the underlying mechanisms by which AX regulates senescence 
      related proteins and signaling pathways remain unclear. Therefore, we used 
      Caenorhabditis elegans (C. elegans) model binding proteomics to reveal AX 
      anti-aging activity and its molecular mechanism. Our results suggest that AX 
      promotes the health and lifespan of C. elegans by improving mobility, reducing 
      the accumulation of age pigments, and increasing resistance to heat stress. In 
      terms of the underlying mechanism, AX helps prolong the life of worms by 
      regulating AGE-1 in the insulin signaling pathway, promoting the transport of 
      DAF-16 into the nucleus and then up-regulating the expression level of DAF-16's 
      downstream proteins (such as superoxide dismutase [Mn] 2 (SOD-3), heat shock 
      proteins (HSPs), glutathione s-transferase (GST-4), etc.). Furthermore, AX may be 
      a relevant response target for activation of dietary restriction pathways in vivo 
      as a dietary restriction mimic. Meanwhile, proteomics data confirmed that there 
      were 15 proteins enriched in the longevity regulation pathway. AX mainly 
      regulates oxidative stress and the aging process by modulating the insulin 
      signaling pathway around DAF-16 as the "hub". In addition to the insulin 
      signaling pathway, other pathways including dietary restriction, AMP-activated 
      protein kinase (AMPK), and mammal target of rapamycin (mTOR) are also dependent 
      on DAF-16. These findings expand and deepen our knowledge of the underlying 
      mechanism by which AX extends the lifespan of C. elegans.
FAU - Liu, Xiaojuan
AU  - Liu X
AUID- ORCID: 0000-0003-2435-5837
AD  - Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, 
      College of Food Science, South China Agricultural University, Guangzhou, 510642, 
      Guangdong, China. liuxj@scau.edu.cn.
AD  - Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China.
FAU - Liu, Han
AU  - Liu H
AD  - Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, 
      College of Food Science, South China Agricultural University, Guangzhou, 510642, 
      Guangdong, China. liuxj@scau.edu.cn.
AD  - Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China.
FAU - Chen, Zhiqing
AU  - Chen Z
AD  - Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, 
      College of Food Science, South China Agricultural University, Guangzhou, 510642, 
      Guangdong, China. liuxj@scau.edu.cn.
AD  - Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China.
FAU - Xiao, Jie
AU  - Xiao J
AUID- ORCID: 0000-0002-4718-1174
AD  - Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, 
      College of Food Science, South China Agricultural University, Guangzhou, 510642, 
      Guangdong, China. liuxj@scau.edu.cn.
AD  - Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China.
FAU - Cao, Yong
AU  - Cao Y
AUID- ORCID: 0000-0002-8334-5324
AD  - Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, 
      College of Food Science, South China Agricultural University, Guangzhou, 510642, 
      Guangdong, China. liuxj@scau.edu.cn.
AD  - Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China.
LA  - eng
PT  - Journal Article
DEP - 20211004
PL  - England
TA  - Food Funct
JT  - Food & function
JID - 101549033
RN  - 0 (Caenorhabditis elegans Proteins)
RN  - 0 (Forkhead Transcription Factors)
RN  - 0 (Insulin)
RN  - 0 (Xanthophylls)
RN  - 0 (daf-16 protein, C elegans)
RN  - 8XPW32PR7I (astaxanthine)
RN  - EC 1.15.1.1 (Sod-3 protein, C elegans)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
SB  - IM
MH  - Aging/drug effects
MH  - Animals
MH  - Caenorhabditis elegans/drug effects/genetics/*physiology
MH  - Caenorhabditis elegans Proteins/genetics/*metabolism
MH  - Cell Nucleus/metabolism
MH  - Cytosol/metabolism
MH  - Forkhead Transcription Factors/*metabolism
MH  - Gene Expression
MH  - Insulin/metabolism
MH  - Longevity/*drug effects
MH  - Mutation
MH  - Proteomics
MH  - Signal Transduction/drug effects
MH  - Stress, Physiological/drug effects
MH  - Superoxide Dismutase/genetics/metabolism
MH  - Xanthophylls/pharmacology
EDAT- 2021/08/17 06:00
MHDA- 2022/01/27 06:00
CRDT- 2021/08/16 08:50
PHST- 2021/08/17 06:00 [pubmed]
PHST- 2022/01/27 06:00 [medline]
PHST- 2021/08/16 08:50 [entrez]
AID - 10.1039/d1fo01069g [doi]
PST - epublish
SO  - Food Funct. 2021 Oct 4;12(19):9098-9110. doi: 10.1039/d1fo01069g.