PMID- 3439718 OWN - NLM STAT- MEDLINE DCOM- 19880401 LR - 20190616 IS - 0077-8923 (Print) IS - 0077-8923 (Linking) VI - 511 DP - 1987 TI - Recovery of HIV-related retroviruses from Italian patients with AIDS or AIDS-related complex and from asymptomatic at-risk individuals. PG - 390-400 AB - Human retroviruses have been detected in supernatants of cultures of Ficoll-enriched lymphocytes from peripheral blood, lymph nodes and bone marrow of (a) 32 out of 42 patients with Acquired Immunodeficiency Syndrome (AIDS), (b) 34 out of 64 patients with AIDS-related Complex (ARC), (c) 9 out of 18 asymptomatic children born from Human Immunodeficiency Virus (HIV) seropositive mothers, and (d) 9 out of 28 asymptomatic drug abusers or hemophiliacs. Virus detection was monitored by assaying culture supernatants for the presence of Mg++-dependent reverse transcriptase (R.T.) activity. A number of these virus-positive sups were passaged repeatedly in cultures of phytohemagglutinin-stimulated and Interleukin-2 (IL-2) treated fresh lymphocytes from healthy blood donors. Occasionally, multiple samples were obtained at varying time intervals from the same patient and consistently yielded detectable retroviral activity. Several isolates were characterized as closely related if not identical to HIV, HTLV-IIIB strain, since cells from either patients' own lymphocyte cultures or subcultures infected with passaged virus were stained in an indirect immunofluorescent assay with both patients sera and monoclonal antibody against p24 antigen of the HTLV-IIIB strain. Representative isolates, grown on fresh lymphocytes of healthy donors and metabolically labelled with 35S-cysteine, were also analyzed in a radioimmunoprecipitation assay (RIPA) against patients' sera to define their antigenic pattern, which was widely superimposable to that obtained with HTLV-IIIB-infected H9 cells. DNA from lymphocytes infected with 2 representative isolates were Southern-blotted and probed with an insert from a plasmid containing the entire genome of the HTLV-IIIB strain. The hybridization patterns were comparable with those obtained with DNA from H9-infected cells. FAU - Rossi, G B AU - Rossi GB AD - Laboratory of Virology, Istituto Superiore di Sanita, Rome, Italy. FAU - Verani, P AU - Verani P FAU - Macchi, B AU - Macchi B FAU - Federico, M AU - Federico M FAU - Orecchia, A AU - Orecchia A FAU - Nicoletti, L AU - Nicoletti L FAU - Butto, S AU - Butto S FAU - Lazzarin, A AU - Lazzarin A FAU - Mariani, G AU - Mariani G FAU - Ippolito, G AU - Ippolito G AU - et al. LA - eng PT - Comparative Study PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - Ann N Y Acad Sci JT - Annals of the New York Academy of Sciences JID - 7506858 SB - IM MH - AIDS-Related Complex/*microbiology MH - Acquired Immunodeficiency Syndrome/*microbiology MH - Blood Transfusion MH - Female MH - HIV/classification/*isolation & purification MH - Hemophilia A/microbiology MH - Homosexuality MH - Humans MH - Infant, Newborn/microbiology MH - Italy MH - Male MH - Pregnancy MH - Pregnancy Complications, Infectious MH - Risk Factors MH - Sexual Behavior MH - Substance-Related Disorders/microbiology MH - Virus Cultivation EDAT- 1987/01/01 00:00 MHDA- 1987/01/01 00:01 CRDT- 1987/01/01 00:00 PHST- 1987/01/01 00:00 [pubmed] PHST- 1987/01/01 00:01 [medline] PHST- 1987/01/01 00:00 [entrez] AID - 10.1111/j.1749-6632.1987.tb36268.x [doi] PST - ppublish SO - Ann N Y Acad Sci. 1987;511:390-400. doi: 10.1111/j.1749-6632.1987.tb36268.x.