PMID- 3439853
OWN - NLM
STAT- MEDLINE
DCOM- 19880406
LR  - 20141120
IS  - 0004-069X (Print)
IS  - 0004-069X (Linking)
VI  - 35
IP  - 4
DP  - 1987
TI  - Preliminary studies on involvement of alpha-1-glycoprotein in the reaction of 
      IgG-3 with leukocytes.
PG  - 413-21
AB  - Alpha-1-glycoprotein (AGP) immobilized on Sepharose and human leukocytes, bound 
      J125 IgG-3 and their fragments F(ab')2 at pH 7.0 and ionic strength 0.15. 
      Increase in concentration of free AGP resulted in a release of the whole IgG-3 
      from AGP-Sepharose and a half of the bound IgG-3 from leukocyte surface. On the 
      other hand, F(ab')2 fragments were released in both cases with 1% AGP. The 
      binding of I125 F(ab')2 to leukocytes was suppressed by the whole non labelled 
      IgG-3 molecules and their fragments F(ab')2. The remaining nonlabelled IgG and 
      their fragments F(ab')2, despite their substantial excess, had no influence upon 
      I125 F(ab')2 binding to leukocytes. AGP:I125 IgG-3 complex of the molar ratio 6:1 
      was shown to bind neither to leukocytes nor to AGP-Sepharose. A suggestion was 
      put forward that AGP, which is anchored in leukocyte membrane, can participate in 
      the binding of IgG-3 subclass. Instead, free AGP enables their release from cell 
      surface.
FAU - Wroblewski, Z
AU  - Wroblewski Z
AD  - Institute of Biochemistry, University of Wroclaw.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Poland
TA  - Arch Immunol Ther Exp (Warsz)
JT  - Archivum immunologiae et therapiae experimentalis
JID - 0114365
RN  - 0 (Immunoglobulin Fab Fragments)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Orosomucoid)
RN  - 9012-36-6 (Sepharose)
SB  - IM
MH  - Adsorption
MH  - Binding Sites
MH  - Cell Membrane/immunology/metabolism
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Immunoglobulin Fab Fragments/metabolism
MH  - Immunoglobulin G/*metabolism
MH  - In Vitro Techniques
MH  - Leukocytes/immunology/*metabolism
MH  - Orosomucoid/*metabolism
MH  - Sepharose
EDAT- 1987/01/01 00:00
MHDA- 1987/01/01 00:01
CRDT- 1987/01/01 00:00
PHST- 1987/01/01 00:00 [pubmed]
PHST- 1987/01/01 00:01 [medline]
PHST- 1987/01/01 00:00 [entrez]
PST - ppublish
SO  - Arch Immunol Ther Exp (Warsz). 1987;35(4):413-21.