PMID- 34399756 OWN - NLM STAT- MEDLINE DCOM- 20211109 LR - 20211109 IS - 1746-6148 (Electronic) IS - 1746-6148 (Linking) VI - 17 IP - 1 DP - 2021 Aug 16 TI - Determination of myrislignan levels in BALB/c mouse plasma by LC-MS/MS and a comparison of its pharmacokinetics after oral and intraperitoneal administration. PG - 275 LID - 10.1186/s12917-021-02990-y [doi] LID - 275 AB - BACKGROUND: Myrislignan is a natural product from Myristica sp. with diverse pharmacological activities. Recently, the anti-Toxoplasma gondii (T. gondii) activity of myrislignan has been proposed, and in vivo studies of its pharmacokinetics in BALB/c mice are necessary to further evaluate the clinical effects of myrislignan. RESULTS: In this study, a sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated to quantify myrislignan levels in mouse plasma using dehydrodiisoeugenol as an internal standard (IS) in positive ion mode. Chromatographic separation of the analytes was achieved using an ACE Ultracore Super C18 analytical column (2.5 mum, 2.1 x 50 mm) at 30 degrees C. A gradient mobile phase consisting of water (0.1 % formic acid) and acetonitrile (0.1 % formic acid) was delivered at a flow rate of 0.4 mL/min. Myrislignan and the IS eluted at 1.42 and 1.71 min, respectively. A good excellent linear response across the concentration range of 1-1000 ng/mL was achieved (r(2) = 0.9973). The lower limit of quantification (LLOQ) was 1 ng/mL, and the inter- and intra-day accuracy and precision of the method showed relative standard deviations (RSDs) less than 10 %. The method was applied to examine the pharmacokinetics of myrislignan in mouse plasma following a single oral administration of 200 mg/kg or intraperitoneal administration of 50 mg/kg myrislignan, and the bioavailability (F) of orally administered myrislignan was only 1.97 % of the bioavailability of intraperitoneally administered myrislignan. CONCLUSIONS: A rapid and sensitive LC-MS/MS method has been was developed, validated and successfully used to determine myrislignan levels in mice after oral or intraperitoneal administration. This study is the first to report the pharmacokinetic parameters of myrislignan in mice and to compare its pharmacokinetics after oral and intraperitoneal administration, which will be useful for further research on the administration of myrislignan in animals and humans. CI - (c) 2021. The Author(s). FAU - Zhang, Jili AU - Zhang J AD - Intensive Care Unit, The Affiliated Hospital of Medical School, Ningbo University, Zhejiang Province, Ningbo, People's Republic of China. AD - School of Medicine, Ningbo University, Ningbo, Zhejiang Province, People's Republic of China. AD - Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Gansu Province, Lanzhou, People's Republic of China. FAU - Si, Hongfei AU - Si H AD - College of Pharmacy, Jinan University, Guangzhou, Guangdong Province, People's Republic of China. FAU - Sun, Jichao AU - Sun J AD - College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang Province, People's Republic of China. FAU - Lv, Kun AU - Lv K AD - School of Business, Ningbo University, Ningbo, Zhejiang Province, People's Republic of China. FAU - Yan, Biqing AU - Yan B AD - Intensive Care Unit, The Affiliated Hospital of Medical School, Ningbo University, Zhejiang Province, Ningbo, People's Republic of China. FAU - Li, Bing AU - Li B AD - Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Gansu Province, Lanzhou, People's Republic of China. FAU - Zhou, Xuzheng AU - Zhou X AD - Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Gansu Province, Lanzhou, People's Republic of China. FAU - Zhang, Jiyu AU - Zhang J AD - Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Gansu Province, Lanzhou, People's Republic of China. zhangjiyu@caas.cn. AD - Key Laboratory of Veterinary Pharmaceutical Development, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, 730050, Lanzhou, People's Republic of China. zhangjiyu@caas.cn. LA - eng GR - CARS-37/the earmarked fund for the China Agriculture Research System/ GR - 2015BAD11B01/the National Science & Technology Pillar Program during the Twelfth Five-year Plan Period/ PT - Journal Article DEP - 20210816 PL - England TA - BMC Vet Res JT - BMC veterinary research JID - 101249759 RN - 0 (2-(4-allyl-2,6-dimethoxyphenoxy)-1-(4-hydroxy-3-methoxyphenyl)propan-1-ol) RN - 0 (Lignans) SB - IM MH - Administration, Oral MH - Animals MH - Area Under Curve MH - BALB 3T3 Cells MH - Biological Availability MH - *Chromatography, Liquid MH - Half-Life MH - Injections, Intraperitoneal/veterinary MH - Lignans/administration & dosage/*blood/*pharmacokinetics MH - Mice MH - Mice, Inbred BALB C MH - Reproducibility of Results MH - Sensitivity and Specificity MH - *Tandem Mass Spectrometry PMC - PMC8365968 OTO - NOTNLM OT - Bioavailability OT - Dehydrodiisoeugenol OT - LC-MS/MS OT - Mouse OT - Myrislignan OT - Pharmacokinetics COIS- The authors declare that they have no competing interests. EDAT- 2021/08/18 06:00 MHDA- 2021/11/10 06:00 PMCR- 2021/08/16 CRDT- 2021/08/17 05:43 PHST- 2021/03/16 00:00 [received] PHST- 2021/08/05 00:00 [accepted] PHST- 2021/08/17 05:43 [entrez] PHST- 2021/08/18 06:00 [pubmed] PHST- 2021/11/10 06:00 [medline] PHST- 2021/08/16 00:00 [pmc-release] AID - 10.1186/s12917-021-02990-y [pii] AID - 2990 [pii] AID - 10.1186/s12917-021-02990-y [doi] PST - epublish SO - BMC Vet Res. 2021 Aug 16;17(1):275. doi: 10.1186/s12917-021-02990-y.