PMID- 34402288
OWN - NLM
STAT- MEDLINE
DCOM- 20210818
LR  - 20210818
IS  - 1001-5302 (Print)
IS  - 1001-5302 (Linking)
VI  - 46
IP  - 14
DP  - 2021 Jul
TI  - [Molecular mechanism of geniposide in regulating GLUT2 glycosylation in 
      pancreatic beta cells].
PG  - 3643-3649
LID - 10.19540/j.cnki.cjcmm.20200118.401 [doi]
AB  - Type 2 diabetes mellitus( T2 DM) is a common chronic metabolic disease 
      characterized by persistent hyperglycemia and insulin resistance. In pancreatic 
      beta-cells,glucose-stimulated insulin secretion( GSIS) plays a pivotal role in 
      maintaining the balance of blood glucose level. Previous studies have shown that 
      geniposide,one of the active components of Gardenia jasminoides,could quickly 
      regulate the absorption and metabolism of glucose,and affect glucose-stimulated 
      insulin secretion in pancreatic beta cells,but the specific mechanism needs to be 
      further explored. Emerging evidence indicated that glycosylation of glucose 
      transporter( GLUT) has played a key role in sensing cell microenvironmental 
      changes and regulating glucose homeostasis in eucaryotic cells. In this study,we 
      studied the effects of geniposide on the key molecules of GLUT2 glycosylation in 
      pancreatic beta cells. The results showed that geniposide could significantly 
      up-regulate the mRNA and protein levels of Glc NAc T-Ⅳa glycosyltransferase( Gn 
      T-Ⅳa) and galectin-9 but had no signi-ficant effect on the expression of 
      clathrin,and geniposide could distinctively regulate the protein level of Gn T-Ⅳa 
      in a short time( 1 h) under the conditions of low and medium glucose 
      concentrations,but had no significant effect on the protein level of galectin-9. 
      In addition,geniposide could also remarkably affect the protein level of 
      glycosylated GLUT2 in a short-time treatment. The above results suggested that 
      geniposide could quickly regulate the protein level of Gn T-Ⅳa,a key molecule of 
      protein glycosylation in INS-1 rat pancreatic betacells and affect the glycosylation 
      of GLUT2. These findings suggested that the regulation of geniposide on glucose 
      absorption,metabolism and glucose-stimulated insulin secretion might be 
      associated with its efficacy in regulating GLUT2 glycosylation and affecting its 
      distribution on the cell membrane and cytoplasm in pancreatic beta cells.
FAU - Jiang, Xiao-Qing
AU  - Jiang XQ
AD  - Chongqing Key Laboratory of Medicinal Chemistry & Molecular Pharmacology 
      Chongqing 400054,China School of Pharmacy & Bioengineering,Chongqing University 
      of Technology Chongqing 400054,China.
FAU - Shen, Shen-Li
AU  - Shen SL
AD  - Chongqing Key Laboratory of Medicinal Chemistry & Molecular Pharmacology 
      Chongqing 400054,China School of Pharmacy & Bioengineering,Chongqing University 
      of Technology Chongqing 400054,China.
FAU - Li, Wei-Zhao
AU  - Li WZ
AD  - Chongqing Key Laboratory of Medicinal Chemistry & Molecular Pharmacology 
      Chongqing 400054,China School of Pharmacy & Bioengineering,Chongqing University 
      of Technology Chongqing 400054,China.
FAU - Xu, Xing-Ke
AU  - Xu XK
AD  - Chongqing Key Laboratory of Medicinal Chemistry & Molecular Pharmacology 
      Chongqing 400054,China School of Pharmacy & Bioengineering,Chongqing University 
      of Technology Chongqing 400054,China.
FAU - Yin, Fei
AU  - Yin F
AD  - Chongqing Key Laboratory of Medicinal Chemistry & Molecular Pharmacology 
      Chongqing 400054,China School of Pharmacy & Bioengineering,Chongqing University 
      of Technology Chongqing 400054,China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhongguo Zhong Yao Za Zhi
JT  - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese 
      materia medica
JID - 8913656
RN  - 0 (Insulin)
RN  - 0 (Iridoids)
RN  - 145295QLXY (geniposide)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - *Diabetes Mellitus, Type 2/drug therapy/genetics/metabolism
MH  - Glucose/metabolism
MH  - Glycosylation
MH  - Insulin/metabolism
MH  - *Insulin-Secreting Cells/metabolism
MH  - Iridoids
MH  - Rats
OTO - NOTNLM
OT  - galectin-9
OT  - geniposide
OT  - glucose transporter 2(GLUT2)
OT  - glycosylation
OT  - glycosyltransferase
EDAT- 2021/08/18 06:00
MHDA- 2021/08/19 06:00
CRDT- 2021/08/17 07:57
PHST- 2021/08/17 07:57 [entrez]
PHST- 2021/08/18 06:00 [pubmed]
PHST- 2021/08/19 06:00 [medline]
AID - 10.19540/j.cnki.cjcmm.20200118.401 [doi]
PST - ppublish
SO  - Zhongguo Zhong Yao Za Zhi. 2021 Jul;46(14):3643-3649. doi: 
      10.19540/j.cnki.cjcmm.20200118.401.