PMID- 34405464
OWN - NLM
STAT- MEDLINE
DCOM- 20210909
LR  - 20210909
IS  - 1530-6860 (Electronic)
IS  - 0892-6638 (Linking)
VI  - 35
IP  - 9
DP  - 2021 Sep
TI  - Paternal high protein diet modulates body composition, insulin sensitivity, 
      epigenetics, and gut microbiota intergenerationally in rats.
PG  - e21847
LID - 10.1096/fj.202100198RR [doi]
AB  - Mounting evidence demonstrates that paternal diet programs offspring metabolism. 
      However, the contribution of a pre-conception paternal high protein (HP) diet to 
      offspring metabolism, gut microbiota, and epigenetic changes remains unclear. 
      Here we show that paternal HP intake in Sprague Dawley rats programs protective 
      metabolic outcomes in offspring. Compared to paternal high fat/sucrose (HF/S), HP 
      diet improved body composition and insulin sensitivity and improved circulating 
      satiety hormones and cecal short-chain fatty acids compared to HF/S and control 
      diet (P < .05). Further, using 16S rRNA gene sequencing to assess gut microbial 
      composition, we observed increased alpha diversity, distinct bacterial 
      clustering, and increased abundance of Bifidobacterium, Akkermansia, Bacteroides, 
      and Marvinbryantia in HP fathers and/or male and female adult offspring. At the 
      epigenetic level, DNMT1and 3b expression was altered intergenerationally. Our 
      study identifies paternal HP diet as a modulator of gut microbial composition, 
      epigenetic markers, and metabolic function intergenerationally.
CI  - (c) 2021 The Authors. The FASEB Journal published by Wiley Periodicals LLC on 
      behalf of Federation of American Societies for Experimental Biology.
FAU - Chleilat, Faye
AU  - Chleilat F
AD  - Faculty of Kinesiology, University of Calgary, Calgary, AB, Canada.
FAU - Schick, Alana
AU  - Schick A
AD  - International Microbiome Centre, Cumming School of Medicine, University of 
      Calgary, Calgary, AB, Canada.
FAU - Deleemans, Julie M
AU  - Deleemans JM
AD  - Division of Medical Science and Psychosocial Oncology, Cumming School of 
      Medicine, University of Calgary, Calgary, AB, Canada.
FAU - Ma, Kyle
AU  - Ma K
AD  - Faculty of Kinesiology, University of Calgary, Calgary, AB, Canada.
FAU - Alukic, Erna
AU  - Alukic E
AD  - Faculty of Kinesiology, University of Calgary, Calgary, AB, Canada.
FAU - Wong, Jolene
AU  - Wong J
AD  - Faculty of Kinesiology, University of Calgary, Calgary, AB, Canada.
FAU - Noye Tuplin, Erin W
AU  - Noye Tuplin EW
AD  - Faculty of Kinesiology, University of Calgary, Calgary, AB, Canada.
FAU - Nettleton, Jodi E
AU  - Nettleton JE
AD  - Faculty of Kinesiology, University of Calgary, Calgary, AB, Canada.
FAU - Reimer, Raylene A
AU  - Reimer RA
AUID- ORCID: 0000-0001-5088-7947
AD  - Faculty of Kinesiology, University of Calgary, Calgary, AB, Canada.
AD  - Department of Biochemistry and Molecular Biology, Cumming School of Medicine, 
      University of Calgary, Calgary, AB, Canada.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - FASEB J
JT  - FASEB journal : official publication of the Federation of American Societies for 
      Experimental Biology
JID - 8804484
RN  - 0 (Dietary Sucrose)
RN  - 0 (Fatty Acids)
RN  - 0 (Hormones)
RN  - 0 (Insulin)
RN  - 0 (RNA, Small Untranslated)
RN  - EC 2.1.1.37 (DNA (Cytosine-5-)-Methyltransferases)
SB  - IM
MH  - Adipose Tissue/metabolism
MH  - Adiposity
MH  - Aging
MH  - Animals
MH  - *Body Composition
MH  - Body Weight
MH  - DNA (Cytosine-5-)-Methyltransferases/metabolism
MH  - Diet, High-Fat
MH  - *Diet, High-Protein
MH  - Dietary Sucrose
MH  - Energy Intake
MH  - Energy Metabolism
MH  - *Epigenesis, Genetic
MH  - *Fathers
MH  - Fatty Acids/metabolism
MH  - Female
MH  - Fertility
MH  - *Gastrointestinal Microbiome
MH  - Glucose Tolerance Test
MH  - Hormones/metabolism
MH  - Insulin/*metabolism
MH  - Insulin Resistance
MH  - Liver/metabolism
MH  - Male
MH  - Organ Size
MH  - *Paternal Exposure
MH  - Pregnancy
MH  - RNA, Small Untranslated/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Satiety Response
MH  - Weaning
OTO - NOTNLM
OT  - 16S rRNA sequencing
OT  - DNA methyltransferase
OT  - body composition
OT  - epigenetics
OT  - gut microbiota
OT  - high protein diet
OT  - insulin sensitivity
OT  - paternal nutrition
EDAT- 2021/08/19 06:00
MHDA- 2021/09/10 06:00
CRDT- 2021/08/18 06:46
PHST- 2021/07/22 00:00 [revised]
PHST- 2021/02/02 00:00 [received]
PHST- 2021/07/23 00:00 [accepted]
PHST- 2021/08/18 06:46 [entrez]
PHST- 2021/08/19 06:00 [pubmed]
PHST- 2021/09/10 06:00 [medline]
AID - 10.1096/fj.202100198RR [doi]
PST - ppublish
SO  - FASEB J. 2021 Sep;35(9):e21847. doi: 10.1096/fj.202100198RR.