PMID- 34407873
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210822
IS  - 2045-3701 (Print)
IS  - 2045-3701 (Electronic)
IS  - 2045-3701 (Linking)
VI  - 11
IP  - 1
DP  - 2021 Aug 19
TI  - JAZF1 heterozygous knockout mice show altered adipose development and metabolism.
PG  - 161
LID - 10.1186/s13578-021-00625-1 [doi]
LID - 161
AB  - BACKGROUND: Juxtaposed with another zinc finger protein 1 (JAZF1) is associated 
      with metabolic disorders, including type 2 diabetes mellitus (T2DM). Several 
      studies showed that JAZF1 and body fat mass are closely related. We attempted to 
      elucidate the JAZF1 functions on adipose development and related metabolism using 
      in vitro and in vivo models. RESULTS: The JAZF1 expression was precisely 
      regulated during adipocyte differentiation of 3T3-L1 preadipocyte and mouse 
      embryonic fibroblasts (MEFs). Homozygous JAZF1 deletion (JAZF1-KO) resulted in 
      impaired adipocyte differentiation in MEF. The JAZF1 role in adipocyte 
      differentiation was demonstrated by the regulation of PPARgamma-a key regulator of 
      adipocyte differentiation. Heterozygous JAZF1 deletion (JAZF1-Het) mice fed a 
      normal diet (ND) or a high-fat diet (HFD) had less adipose tissue mass and 
      impaired glucose homeostasis than the control (JAZF1-Cont) mice. However, other 
      metabolic organs, such as brown adipose tissue and liver, were negligible effect 
      on JAZF1 deficiency. CONCLUSION: Our findings emphasized the JAZF1 role in 
      adipocyte differentiation and related metabolism through the heterozygous 
      knockout mice. This study provides new insights into the JAZF1 function in 
      adipose development and metabolism, informing strategies for treating obesity and 
      related metabolic disorders.
CI  - (c) 2021. The Author(s).
FAU - Jeong, Jain
AU  - Jeong J
AD  - Digestive Diseases Section, Department of Internal Medicine, Yale University 
      School of Medicine, New Haven, CT, USA.
FAU - Jang, Soyoung
AU  - Jang S
AD  - School of Life Sciences, BK21 FOUR KNU Creative BioResearch, Kyungpook National 
      University, Daegu, 41566, Republic of Korea.
FAU - Park, Song
AU  - Park S
AD  - Core Protein Resources Center, DGIST, Daegu, 42988, Republic of Korea.
AD  - Department of Brain and Cognitive Sciences, DGIST, Daegu, Republic of Korea.
FAU - Kwon, Wookbong
AU  - Kwon W
AD  - Division of Biotechnology, DGIST, Daegu, Republic of Korea.
FAU - Kim, Si-Yong
AU  - Kim SY
AD  - School of Life Sciences, BK21 FOUR KNU Creative BioResearch, Kyungpook National 
      University, Daegu, 41566, Republic of Korea.
FAU - Jang, Soyoen
AU  - Jang S
AD  - School of Life Sciences, BK21 FOUR KNU Creative BioResearch, Kyungpook National 
      University, Daegu, 41566, Republic of Korea.
FAU - Ko, Jiwon
AU  - Ko J
AD  - School of Life Sciences, BK21 FOUR KNU Creative BioResearch, Kyungpook National 
      University, Daegu, 41566, Republic of Korea.
FAU - Park, Si Jun
AU  - Park SJ
AD  - School of Life Sciences, BK21 FOUR KNU Creative BioResearch, Kyungpook National 
      University, Daegu, 41566, Republic of Korea.
FAU - Lim, Su-Geun
AU  - Lim SG
AD  - School of Life Sciences, BK21 FOUR KNU Creative BioResearch, Kyungpook National 
      University, Daegu, 41566, Republic of Korea.
FAU - Yoon, Duhak
AU  - Yoon D
AD  - Department of Animal Science, Kyungpook National University, Daegu, 37224, 
      Republic of Korea.
FAU - Yi, Junkoo
AU  - Yi J
AD  - Gyeongsangbukdo Livestock Research Institute, Yeongju, Republic of Korea.
FAU - Lee, Sanggyu
AU  - Lee S
AD  - School of Life Sciences, BK21 FOUR KNU Creative BioResearch, Kyungpook National 
      University, Daegu, 41566, Republic of Korea.
FAU - Kim, Myoung Ok
AU  - Kim MO
AD  - School of Animal Science and Biotechnology, Kyungpook National University, Daegu, 
      Korea.
FAU - Choi, Seong-Kyoon
AU  - Choi SK
AD  - Core Protein Resources Center, DGIST, Daegu, 42988, Republic of Korea. 
      cskbest@dgist.ac.kr.
AD  - Division of Biotechnology, DGIST, Daegu, Republic of Korea. cskbest@dgist.ac.kr.
FAU - Ryoo, Zae Young
AU  - Ryoo ZY
AUID- ORCID: 0000-0001-6993-3624
AD  - School of Life Sciences, BK21 FOUR KNU Creative BioResearch, Kyungpook National 
      University, Daegu, 41566, Republic of Korea. jaewoong64@knu.ac.kr.
LA  - eng
GR  - 2020R1I1A2075315/National Research Foundation of Korea/
GR  - 2020R1I1A1A01074542/National Research Foundation of Korea/
GR  - NRF-2021R1F1A1045382/National Research Foundation of Korea/
GR  - 2020R1A4A1018280/National Research Foundation of Korea/
GR  - 2020010096/Daegu Gyeongbuk Institute of Science and Technology/
PT  - Journal Article
DEP - 20210819
PL  - England
TA  - Cell Biosci
JT  - Cell & bioscience
JID - 101561195
PMC - PMC8375039
OTO - NOTNLM
OT  - Adipocyte differentiation
OT  - Adipogenesis
OT  - Glucose homeostasis
OT  - Insulin resistance
OT  - JAZF1
OT  - PPARgamma
COIS- The authors declare they have no competing interests.
EDAT- 2021/08/20 06:00
MHDA- 2021/08/20 06:01
PMCR- 2021/08/19
CRDT- 2021/08/19 05:40
PHST- 2021/02/12 00:00 [received]
PHST- 2021/06/10 00:00 [accepted]
PHST- 2021/08/19 05:40 [entrez]
PHST- 2021/08/20 06:00 [pubmed]
PHST- 2021/08/20 06:01 [medline]
PHST- 2021/08/19 00:00 [pmc-release]
AID - 10.1186/s13578-021-00625-1 [pii]
AID - 625 [pii]
AID - 10.1186/s13578-021-00625-1 [doi]
PST - epublish
SO  - Cell Biosci. 2021 Aug 19;11(1):161. doi: 10.1186/s13578-021-00625-1.