PMID- 34408262
OWN - NLM
STAT- MEDLINE
DCOM- 20220321
LR  - 20240226
IS  - 2092-6413 (Electronic)
IS  - 1226-3613 (Print)
IS  - 1226-3613 (Linking)
VI  - 53
IP  - 8
DP  - 2021 Aug
TI  - LINC00319 promotes cancer stem cell-like properties in laryngeal squamous cell 
      carcinoma via E2F1-mediated upregulation of HMGB3.
PG  - 1218-1228
LID - 10.1038/s12276-021-00647-2 [doi]
AB  - Laryngeal squamous cell carcinoma (LSCC) is one of the most common subtypes of 
      head and neck malignancies worldwide. Long intervening/intergenic noncoding RNAs 
      (LINCRNAs) have been recently implicated in various biological processes that 
      take place in the setting of laryngeal cancer, but the regulatory role of 
      LINC00319 in LSCC remains largely unknown. The current study aimed to elucidate 
      the regulatory effect of LINC00319 on the development and progression of LSCC via 
      high-mobility group box 3 (HMGB3). Microarray-based analysis was initially 
      conducted to identify differentially expressed long noncoding RNAs, after which 
      the expression of LINC00319 and HMGB3 in LSCC tissues and cells was determined 
      accordingly. CD133(+)CD144(+) TU177 cells were subsequently isolated and 
      transfected with LINC00319 overexpression vector (oe-LINC00319), short hairpin 
      RNA (sh)-LINC00319, sh-HMGB3, sh-E2F transcription factor 1 (E2F1), and oe-E2F1, 
      as well as their corresponding controls. The proliferative, invasion, 
      self-renewal, and tumorigenic abilities of CD133(+)CD144(+) TU177 cells were then 
      evaluated. Our in vitro findings were further confirmed following subcutaneous 
      injection of cells expressing the corresponding plasmids into nude mice. 
      LINC00319 and HMGB3 expressions were elevated in LSCC cells and tissues. 
      LINC00319 increased HMGB3 expression by recruiting E2F1. Furthermore, the 
      stimulatory role of LINC00319 on the proliferation, invasion, self-renewal 
      ability, and tumorigenicity of CD133(+)CD144(+) TU177 cells was achieved by 
      upregulating HMGB3 via recruitment of E2F1. The in vitro findings were also 
      confirmed by in vivo experiments. Taken together, these data show that 
      downregulating LINC00319 in CD133(+)CD144(+) TU177 cells may serve as a potential 
      anticancer regimen by inhibiting the proliferation and invasion of cancer stem 
      cells in LSCC.
CI  - (c) 2021. The Author(s).
FAU - Yuan, Linlin
AU  - Yuan L
AD  - Department of Otorhinolaryngology Head and Neck Surgery, The First Affiliated 
      Hospital of Zhengzhou University, 450052, Zhengzhou, People's Republic of China.
FAU - Tian, Xiufen
AU  - Tian X
AD  - Department of Otorhinolaryngology Head and Neck Surgery, The First Affiliated 
      Hospital of Zhengzhou University, 450052, Zhengzhou, People's Republic of China.
FAU - Zhang, Yanfei
AU  - Zhang Y
AD  - Department of Otorhinolaryngology Head and Neck Surgery, The First Affiliated 
      Hospital of Zhengzhou University, 450052, Zhengzhou, People's Republic of China.
FAU - Huang, Xinhui
AU  - Huang X
AD  - Department of Otorhinolaryngology Head and Neck Surgery, The First Affiliated 
      Hospital of Zhengzhou University, 450052, Zhengzhou, People's Republic of China.
FAU - Li, Qing
AU  - Li Q
AD  - Department of Pathology, The Third Affiliated Hospital of Soochow University 
      (Changzhou City No. 1 People's Hospital), 215006, Changzhou, People's Republic of 
      China.
FAU - Li, Wencai
AU  - Li W
AD  - Department of Otorhinolaryngology Head and Neck Surgery, The First Affiliated 
      Hospital of Zhengzhou University, 450052, Zhengzhou, People's Republic of China. 
      drli_liwencai@163.com.
FAU - Li, Shenglei
AU  - Li S
AD  - Department of Otorhinolaryngology Head and Neck Surgery, The First Affiliated 
      Hospital of Zhengzhou University, 450052, Zhengzhou, People's Republic of China. 
      lishenglei@zzu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210818
PL  - United States
TA  - Exp Mol Med
JT  - Experimental & molecular medicine
JID - 9607880
RN  - 0 (E2F1 Transcription Factor)
RN  - 0 (E2F1 protein, human)
RN  - 0 (HMGB3 Protein)
RN  - 0 (RNA, Long Noncoding)
SB  - IM
MH  - Animals
MH  - Carcinoma, Squamous Cell/*genetics
MH  - Cell Line, Tumor
MH  - Cell Proliferation/genetics
MH  - E2F1 Transcription Factor/*genetics/metabolism
MH  - *Gene Expression Regulation, Neoplastic
MH  - HMGB3 Protein/*genetics/metabolism
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics/pathology
MH  - Mice, Inbred BALB C
MH  - Mice, Nude
MH  - Models, Biological
MH  - Neoplastic Stem Cells/metabolism/*pathology
MH  - Prognosis
MH  - RNA, Long Noncoding/genetics/*metabolism
MH  - Up-Regulation/*genetics
MH  - Mice
PMC - PMC8417254
COIS- The authors declare no competing interests.
EDAT- 2021/08/20 06:00
MHDA- 2022/03/22 06:00
PMCR- 2021/08/18
CRDT- 2021/08/19 06:29
PHST- 2019/04/20 00:00 [received]
PHST- 2021/05/27 00:00 [accepted]
PHST- 2021/04/09 00:00 [revised]
PHST- 2021/08/20 06:00 [pubmed]
PHST- 2022/03/22 06:00 [medline]
PHST- 2021/08/19 06:29 [entrez]
PHST- 2021/08/18 00:00 [pmc-release]
AID - 10.1038/s12276-021-00647-2 [pii]
AID - 647 [pii]
AID - 10.1038/s12276-021-00647-2 [doi]
PST - ppublish
SO  - Exp Mol Med. 2021 Aug;53(8):1218-1228. doi: 10.1038/s12276-021-00647-2. Epub 2021 
      Aug 18.