PMID- 34408624
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210820
IS  - 1662-4548 (Print)
IS  - 1662-453X (Electronic)
IS  - 1662-453X (Linking)
VI  - 15
DP  - 2021
TI  - Diurnal Variation in Trigeminal Pain Sensitivity in Mice.
PG  - 703440
LID - 10.3389/fnins.2021.703440 [doi]
LID - 703440
AB  - Management of time and circadian disruption is an extremely important factor in 
      basic research on pain and analgesia. Although pain is known to vary throughout 
      the day, the mechanism underlying this circadian variation remains largely 
      unknown. In this study, we hypothesized that the process of pain transmission to 
      the central nervous system (after receiving nociceptive stimuli from outside the 
      body) would show day-night differences. Ten-week-old male mice were kept under a 
      strict 12/12-h light/dark cycle for at least 10 days. Formalin was then injected 
      into the second branch region of the trigeminal nerve and the duration of 
      pain-related behaviors (PRBs) was assessed. Immunohistochemical staining was then 
      performed, and the c-Fos-immunopositive cells in the trigeminal spinal tract 
      subnucleus caudalis (Sp5C) were counted. The results showed that the duration of 
      PRBs was longer and the number of c-Fos immunopositive cells in the Sp5C was 
      higher at nighttime than during the day. In addition, the trigeminal ganglia (TG) 
      were extracted from the mice and examined by quantitative real-time PCR to 
      evaluate the daytime and nighttime expression of nociceptive receptors. The 
      results showed that the mRNA expression of transient receptor potential ankyrin 1 
      in the TG was significantly higher at night than during the day. These results 
      suggest that pain in the trigeminal nerve region is more intense at nighttime, 
      when rodents are active, than during the daytime, partly due to differences in 
      nociceptor expression.
CI  - Copyright (c) 2021 Niiro, Ohno, Yamagata, Yamagata, Tomita, Kuramoto, Oda, 
      Nakamura, Nakamura and Sugimura.
FAU - Niiro, Ayako
AU  - Niiro A
AD  - Department of Dental Anesthesiology, Graduate School of Medical and Dental 
      Sciences, Kagoshima University, Kagoshima, Japan.
FAU - Ohno, Sachi N
AU  - Ohno SN
AD  - Department of Dental Anesthesiology, Graduate School of Medical and Dental 
      Sciences, Kagoshima University, Kagoshima, Japan.
FAU - Yamagata, Kanae A
AU  - Yamagata KA
AD  - Department of Dental Anesthesiology, Graduate School of Medical and Dental 
      Sciences, Kagoshima University, Kagoshima, Japan.
FAU - Yamagata, Kazuaki
AU  - Yamagata K
AD  - Department of Dental Anesthesiology, Graduate School of Medical and Dental 
      Sciences, Kagoshima University, Kagoshima, Japan.
FAU - Tomita, Kazuo
AU  - Tomita K
AD  - Department of Applied Pharmacology, Graduate School of Medical and Dental 
      Sciences, Kagoshima University, Kagoshima, Japan.
FAU - Kuramoto, Eriko
AU  - Kuramoto E
AD  - Department of Oral Anatomy and Cell Biology, Graduate School of Medical and 
      Dental Sciences, Kagoshima University, Kagoshima, Japan.
FAU - Oda, Yoshiaki
AU  - Oda Y
AD  - Unit of Basic Medical Sciences, Department of Oral Chrono-Physiology, Graduate 
      School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan.
FAU - Nakamura, Takahiro J
AU  - Nakamura TJ
AD  - Laboratory of Animal Physiology, School of Agriculture, Meiji University, 
      Kawasaki, Japan.
FAU - Nakamura, Wataru
AU  - Nakamura W
AD  - Unit of Basic Medical Sciences, Department of Oral Chrono-Physiology, Graduate 
      School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan.
FAU - Sugimura, Mitsutaka
AU  - Sugimura M
AD  - Department of Dental Anesthesiology, Graduate School of Medical and Dental 
      Sciences, Kagoshima University, Kagoshima, Japan.
LA  - eng
PT  - Journal Article
DEP - 20210802
PL  - Switzerland
TA  - Front Neurosci
JT  - Frontiers in neuroscience
JID - 101478481
PMC - PMC8365185
OTO - NOTNLM
OT  - TRPA1
OT  - day-night difference
OT  - formalin test
OT  - pain
OT  - trigeminal nervous system
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2021/08/20 06:00
MHDA- 2021/08/20 06:01
PMCR- 2021/01/01
CRDT- 2021/08/19 06:39
PHST- 2021/04/30 00:00 [received]
PHST- 2021/07/12 00:00 [accepted]
PHST- 2021/08/19 06:39 [entrez]
PHST- 2021/08/20 06:00 [pubmed]
PHST- 2021/08/20 06:01 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - 10.3389/fnins.2021.703440 [doi]
PST - epublish
SO  - Front Neurosci. 2021 Aug 2;15:703440. doi: 10.3389/fnins.2021.703440. eCollection 
      2021.