PMID- 34410124
OWN - NLM
STAT- MEDLINE
DCOM- 20210902
LR  - 20210902
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Linking)
VI  - 69
IP  - 34
DP  - 2021 Sep 1
TI  - Primary Bitter Taste of Citrus is Linked to a Functional Allele of the 
      1,2-Rhamnosyltransferase Gene Originating from Citrus grandis.
PG  - 9869-9882
LID - 10.1021/acs.jafc.1c01211 [doi]
AB  - 1,2-Rhamnosyltransferase (1,2RhaT) catalyzes the final step of production of 
      flavanone neohesperidoside (FNH) that is responsible for the primary bitter taste 
      of citrus fruits. In this study, species-specific flavonoid profiles were 
      determined in 87 Citrus accessions by identifying eight main flavanone glycosides 
      (FGs). Accumulation of FNHs was completely correlated to the presence of the 
      1,2RhaT gene in 87 citrus accessions analyzed using a novel 1,2RhaT-specific DNA 
      marker. Pummelo (Citrus grandis) was identified as the genetic origin for a 
      function allele of 1,2RhaT that underpinned FNH-bitterness in modern citrus 
      cultivars. In addition, genes encoding six MYB and five bHLH transcription 
      factors were shown to coexpress with 1,2RhaT and other flavonoid pathway genes 
      related to FNH accumulation, indicating that these transcription factors may 
      affect the fruit taste of citrus. This study provides a better understanding of 
      bitterness formation in Citrus varieties and a genetic marker for the early 
      selection of nonbitterness lines in citrus breeding programs.
FAU - Chen, Jiajing
AU  - Chen J
AD  - Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of 
      Horticulture and Forestry, Huazhong Agricultural University, Wuhan 430070, P. R. 
      China.
FAU - Li, Gu
AU  - Li G
AD  - Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of 
      Horticulture and Forestry, Huazhong Agricultural University, Wuhan 430070, P. R. 
      China.
FAU - Zhang, Haipeng
AU  - Zhang H
AD  - Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of 
      Horticulture and Forestry, Huazhong Agricultural University, Wuhan 430070, P. R. 
      China.
FAU - Yuan, Ziyu
AU  - Yuan Z
AD  - Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of 
      Horticulture and Forestry, Huazhong Agricultural University, Wuhan 430070, P. R. 
      China.
FAU - Li, Wenyun
AU  - Li W
AD  - Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of 
      Horticulture and Forestry, Huazhong Agricultural University, Wuhan 430070, P. R. 
      China.
FAU - Peng, Zhaoxin
AU  - Peng Z
AD  - Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of 
      Horticulture and Forestry, Huazhong Agricultural University, Wuhan 430070, P. R. 
      China.
FAU - Shi, Meiyan
AU  - Shi M
AD  - Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of 
      Horticulture and Forestry, Huazhong Agricultural University, Wuhan 430070, P. R. 
      China.
FAU - Ding, Wenyu
AU  - Ding W
AD  - Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of 
      Horticulture and Forestry, Huazhong Agricultural University, Wuhan 430070, P. R. 
      China.
FAU - Zhang, Huixian
AU  - Zhang H
AD  - Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of 
      Horticulture and Forestry, Huazhong Agricultural University, Wuhan 430070, P. R. 
      China.
FAU - Cheng, Yunjiang
AU  - Cheng Y
AD  - Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of 
      Horticulture and Forestry, Huazhong Agricultural University, Wuhan 430070, P. R. 
      China.
FAU - Yao, Jia-Long
AU  - Yao JL
AD  - The New Zealand Institute for Plant & Food Research Limited, Private Bag 92169, 
      Auckland 1142, New Zealand.
FAU - Xu, Juan
AU  - Xu J
AUID- ORCID: 0000-0003-4623-9343
AD  - Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of 
      Horticulture and Forestry, Huazhong Agricultural University, Wuhan 430070, P. R. 
      China.
LA  - eng
PT  - Journal Article
DEP - 20210819
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (Flavonoids)
SB  - IM
MH  - Alleles
MH  - *Citrus/genetics
MH  - Flavonoids
MH  - Plant Breeding
MH  - Taste
OTO - NOTNLM
OT  - citrus
OT  - flavanone glycoside
OT  - flavonoid
OT  - neohesperidoside
OT  - rutinoside
OT  - transcription factors
EDAT- 2021/08/20 06:00
MHDA- 2021/09/03 06:00
CRDT- 2021/08/19 12:12
PHST- 2021/08/20 06:00 [pubmed]
PHST- 2021/09/03 06:00 [medline]
PHST- 2021/08/19 12:12 [entrez]
AID - 10.1021/acs.jafc.1c01211 [doi]
PST - ppublish
SO  - J Agric Food Chem. 2021 Sep 1;69(34):9869-9882. doi: 10.1021/acs.jafc.1c01211. 
      Epub 2021 Aug 19.