PMID- 34414170
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210821
IS  - 2296-4185 (Print)
IS  - 2296-4185 (Electronic)
IS  - 2296-4185 (Linking)
VI  - 9
DP  - 2021
TI  - Study on a 3D-Bioprinted Tissue Model of Self-Assembled Nanopeptide Hydrogels 
      Combined With Adipose-Derived Mesenchymal Stem Cells.
PG  - 663120
LID - 10.3389/fbioe.2021.663120 [doi]
LID - 663120
AB  - Objective: This study aimed to observe the cell growth status and 
      multidirectional differentiation ability in a 3D-bioprinted tissue model of 
      self-assembled nanopeptides and human adipose-derived mesenchymal stem cells 
      (Ad-MSCs). Methods: Primary Ad-MSCs were isolated, cultured, and identified by 
      flow cytometry. Tissue models were printed via 3D bioprinting technology using a 
      "biological ink" consisting of a mixed solution of self-assembled nanopeptides 
      and Ad-MSCs. Ad-MSCs were induced into osteogenic, adipogenic, and endothelial 
      differentiation and compared with the control groups by staining. Results: The 
      nanopeptide fiber was 10-30 nm in diameter and 200-500 nm in length under the 
      atomic-force microscope. It had the characteristics of nano-scale materials. Flow 
      cytometry showed that the isolated and cultured cells were positive for CD29 
      (98.51%), CD90 (97.87%), and CD166 (98.32%) but did not express CD31 (1.58%), 
      CD34 (2.42%), CD45 (2.95%), or human leukocyte antigen (HLA)-DR (0.53%), 
      consistent with the immunophenotype of Ad-MSCs. Then, a tissue model was printed 
      using the biological ink, followed by induction of differentiation of Ad-MSCs 
      within the tissue model. Alizarin red S staining showed the formation of calcium 
      nodules in the osteogenesis induction experimental group, and oil red O stained 
      lipid droplets in Ad-MSCs in the adipogenesis induction experimental group, 
      whereas the two control groups were not stained. Conclusion: Ad-MSCs from primary 
      cultures have the characteristics of stem cells. Self-assembled nanopeptide 
      hydrogel is a good tissue engineering material that can serve as an extracellular 
      matrix. Ad-MSCs in the 3D-printed tissue model using a biological ink consisting 
      of a mixed solution of self-assembled nanopeptides and Ad-MSCs grew well and 
      still had strong differentiation ability.
CI  - Copyright (c) 2021 Zhou, Tian, Yi, Fan, Shao, Wu and Sun.
FAU - Zhou, Guanzhou
AU  - Zhou G
AD  - Department of General Surgery, Qilu Hospital of Shandong University, Jinan, 
      China.
AD  - Department of General Surgery, Zibo Central Hospital, Zibo, China.
FAU - Tian, Ailing
AU  - Tian A
AD  - Department of General Surgery, Qilu Hospital of Shandong University, Jinan, 
      China.
FAU - Yi, Xin
AU  - Yi X
AD  - Department of General Surgery, Qilu Hospital of Shandong University, Jinan, 
      China.
FAU - Fan, Lufeng
AU  - Fan L
AD  - Department of General Surgery, Qilu Hospital of Shandong University, Jinan, 
      China.
FAU - Shao, Wenchong
AU  - Shao W
AD  - Department of General Surgery, Qilu Hospital of Shandong University, Jinan, 
      China.
FAU - Wu, Han
AU  - Wu H
AD  - Department of General Surgery, Qilu Hospital of Shandong University, Jinan, 
      China.
FAU - Sun, Nianfeng
AU  - Sun N
AD  - Department of General Surgery, Qilu Hospital of Shandong University, Jinan, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20210803
PL  - Switzerland
TA  - Front Bioeng Biotechnol
JT  - Frontiers in bioengineering and biotechnology
JID - 101632513
PMC - PMC8369258
OTO - NOTNLM
OT  - 3D bioprinting
OT  - adipose-derived mesenchymal stem cells
OT  - hydrogels
OT  - self-assembled nanopeptides
OT  - tissue engineering
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2021/08/21 06:00
MHDA- 2021/08/21 06:01
PMCR- 2021/01/01
CRDT- 2021/08/20 07:07
PHST- 2021/02/02 00:00 [received]
PHST- 2021/07/07 00:00 [accepted]
PHST- 2021/08/20 07:07 [entrez]
PHST- 2021/08/21 06:00 [pubmed]
PHST- 2021/08/21 06:01 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - 663120 [pii]
AID - 10.3389/fbioe.2021.663120 [doi]
PST - epublish
SO  - Front Bioeng Biotechnol. 2021 Aug 3;9:663120. doi: 10.3389/fbioe.2021.663120. 
      eCollection 2021.