PMID- 34414940
OWN - NLM
STAT- MEDLINE
DCOM- 20210902
LR  - 20230103
IS  - 1536-5964 (Electronic)
IS  - 0025-7974 (Print)
IS  - 0025-7974 (Linking)
VI  - 100
IP  - 33
DP  - 2021 Aug 20
TI  - Levels of circulating GRP78 and CHOP in endoplasmic reticulum stress pathways in 
      Chinese type 2 diabetic kidney disease patients.
PG  - e26879
LID - 10.1097/MD.0000000000026879 [doi]
LID - e26879
AB  - The current study aimed to investigate circulating glucose-regulated protein 78 
      (GRP78) as well as CCAAT/enhancer-binding protein homologous protein (CHOP) 
      concentrations in Chinese type 2 diabetes mellitus (T2DM) patients, especially 
      those with microalbuminuria. We recruited 67 patients with T2DM and 63 control 
      subjects. We determined circulating GRP78 and CHOP concentrations by ELISA, 
      collected anthropometric data, and measured biochemical parameters in a clinical 
      laboratory. Compared with control groups, patients with T2DM showed decreased 
      circulating levels of GRP78 (0.21 [0.16-0.24] vs 0.16 [0.16-0.19] ng/mL, P < .01) 
      and CHOP ([0.29 +/- 0.02] vs [0.27 +/- 0.03]ng/mL, P < .01). Reduction in circulating 
      GRP78 and CHOP levels was more pronounced in patients with more severe categories 
      of albuminuria. Amounts of circulating GRP78 correlated directly with serum 
      fasting c-peptide, cystatin-c (Cys-c), creatinine (Cr), blood urea nitrogen 
      (BUN), and uric acid, and inversely with glomerular filtration rates. Circulating 
      CHOP level was positively correlated with age, Cr, BUN, Cys-c, and urinary 
      microalbumin/creatinine (UmALB/Cr). Circulating GRP78 was predicted independently 
      by Cr, BUN, serum uric acid, estimated glomerular filtration rate, and Cys-c, 
      while CHOP depended on age, Cr, BUN, estimated glomerular filtration rate, 
      UmALB/Cr, and Cys-c. After controlling for confounding factors, circulating GRP78 
      and CHOP expression were significantly associated with diabetic kidney disease 
      (binary logistic regression, P < .01). Patients with T2DM showed increased 
      circulating GRP78 and CHOP concentrations. Receiver operating characteristic 
      areas under the curve for predicting diabetic kidney disease based on GRP78 and 
      CHOP were 0.686 (95% CI: 0.558-0.813) and 0.670 (0.524-0.816), respectively.
CI  - Copyright (c) 2021 the Author(s). Published by Wolters Kluwer Health, Inc.
FAU - Ma, Ning
AU  - Ma N
AD  - Department of Endocrinology and Metabolism, Lianyungang No1 People's Hospital, 6 
      Zhenghua Road, Lianyungang, Jiangsu, China.
AD  - Department of Endocrinology and Metabolism, First Affiliated Hospital of Soochow 
      University, 188 Shizi Road, Suzhou, Jiangsu, China.
FAU - Xu, Ning
AU  - Xu N
AD  - Department of Endocrinology and Metabolism, Lianyungang No1 People's Hospital, 6 
      Zhenghua Road, Lianyungang, Jiangsu, China.
FAU - Yin, Dong
AU  - Yin D
AD  - Department of Endocrinology and Metabolism, Lianyungang No1 People's Hospital, 6 
      Zhenghua Road, Lianyungang, Jiangsu, China.
FAU - Zheng, Ping
AU  - Zheng P
AD  - Department of Endocrinology and Metabolism, Lianyungang No1 People's Hospital, 6 
      Zhenghua Road, Lianyungang, Jiangsu, China.
FAU - Liu, Weiwei
AU  - Liu W
AD  - Department of Endocrinology and Metabolism, Lianyungang No1 People's Hospital, 6 
      Zhenghua Road, Lianyungang, Jiangsu, China.
FAU - Wang, Guofeng
AU  - Wang G
AD  - Department of Endocrinology and Metabolism, Lianyungang No1 People's Hospital, 6 
      Zhenghua Road, Lianyungang, Jiangsu, China.
FAU - Hui, Yuan
AU  - Hui Y
AD  - Department of Endocrinology and Metabolism, Lianyungang No1 People's Hospital, 6 
      Zhenghua Road, Lianyungang, Jiangsu, China.
FAU - Han, Guanjun
AU  - Han G
AD  - Department of Endocrinology and Metabolism, Lianyungang No1 People's Hospital, 6 
      Zhenghua Road, Lianyungang, Jiangsu, China.
FAU - Yang, Chuanhui
AU  - Yang C
AD  - Department of Endocrinology and Metabolism, Lianyungang No1 People's Hospital, 6 
      Zhenghua Road, Lianyungang, Jiangsu, China.
FAU - Cheng, Xingbo
AU  - Cheng X
AUID- ORCID: 0000-0001-7570-3120
AD  - Department of Endocrinology and Metabolism, First Affiliated Hospital of Soochow 
      University, 188 Shizi Road, Suzhou, Jiangsu, China.
LA  - eng
GR  - Z2018021/Jiangsu Provincial Commission of Health and Family Planning/
GR  - ZD1802/Lianyungang Commission Health Foundation/
PT  - Journal Article
PL  - United States
TA  - Medicine (Baltimore)
JT  - Medicine
JID - 2985248R
RN  - 0 (DDIT3 protein, human)
RN  - 0 (Endoplasmic Reticulum Chaperone BiP)
RN  - 0 (HSPA5 protein, human)
RN  - 0 (Heat-Shock Proteins)
RN  - 147336-12-7 (Transcription Factor CHOP)
SB  - IM
MH  - Aged
MH  - Asian People
MH  - Cross-Sectional Studies
MH  - Diabetes Mellitus, Type 2/*blood
MH  - Diabetic Nephropathies/*blood
MH  - Endoplasmic Reticulum Chaperone BiP
MH  - *Endoplasmic Reticulum Stress
MH  - Female
MH  - Heat-Shock Proteins/*blood
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Transcription Factor CHOP/*blood
PMC - PMC8376381
COIS- The authors have no conflicts of interest to disclose.
EDAT- 2021/08/21 06:00
MHDA- 2021/09/03 06:00
PMCR- 2021/08/20
CRDT- 2021/08/20 09:05
PHST- 2021/02/11 00:00 [received]
PHST- 2021/07/22 00:00 [accepted]
PHST- 2021/08/20 09:05 [entrez]
PHST- 2021/08/21 06:00 [pubmed]
PHST- 2021/09/03 06:00 [medline]
PHST- 2021/08/20 00:00 [pmc-release]
AID - 00005792-202108200-00010 [pii]
AID - MD-D-21-01145 [pii]
AID - 10.1097/MD.0000000000026879 [doi]
PST - ppublish
SO  - Medicine (Baltimore). 2021 Aug 20;100(33):e26879. doi: 
      10.1097/MD.0000000000026879.