PMID- 34416335 OWN - NLM STAT- MEDLINE DCOM- 20211020 LR - 20211020 IS - 1873-6815 (Electronic) IS - 0531-5565 (Linking) VI - 154 DP - 2021 Oct 15 TI - Prolonged caloric restriction ameliorates age-related atrophy in slow and fast muscle fibers of rat soleus muscle. PG - 111519 LID - S0531-5565(21)00301-6 [pii] LID - 10.1016/j.exger.2021.111519 [doi] AB - Aging causes loss of skeletal muscle mass and function, which is called sarcopenia. While sarcopenia impairs the quality of life of older adults and is a major factor in long-term hospitalization, its detailed pathogenic mechanism and preventive measures remain to be identified. Caloric restriction (CR) suppresses age-related physiological and pathological changes in many species and prolongs the average and healthy life expectancy. It has recently been reported that CR suppresses the onset of sarcopenia; however, few studies have analyzed the effects of long-term CR on age-related skeletal muscle atrophy. Thus, we investigated the aging and CR effects on soleus (SOL) muscles of 9-, 24-, and 29-month-old ad libitum-fed rats (9AL, 24AL, and 29AL, respectively) and of 29-month-old CR (29CR) rats. The total muscle cross sectional area (mCSA) of the entire SOL muscle significantly decreased in the 29AL rats, but not in the 24AL rats, compared with the 9AL rats. SOL muscle of the 29AL rats exhibited marked muscle fiber atrophy and increases in the number of muscle fibers with a central nucleus, in fibrosis, and in adipocyte infiltration. Additionally, although the decrease in the single muscle fiber cross-sectional area (fCSA) and the muscle fibers' number occurred in both slow-type and fast-type muscle fibers, the degree of atrophy was more remarkable in the fast-type fibers. However, CR suppressed the muscle fiber atrophy observed in the 29AL rats' SOL muscle by preserving the mCSA and the number of muscle fibers that declined with aging, and by decreasing the number of muscle fibers with a central nucleus, fibrosis and denervated muscle fibers. Overall, these results revealed that advanced aging separately reduces the number and fCSA of each muscle fiber type, but long-term CR can ameliorate this age-related sarcopenic muscle atrophy. CI - Copyright (c) 2021 The Authors. Published by Elsevier Inc. All rights reserved. FAU - Mizunoe, Yuhei AU - Mizunoe Y AD - Department of Internal Medicine (Endocrinology and Metabolism), Faculty of Medicine, University of Tsukuba, Tsukuba, Japan. FAU - Kobayashi, Masaki AU - Kobayashi M AD - Laboratory of Molecular Pathology & Metabolic Disease, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Chiba, Japan. FAU - Saito, Hiroki AU - Saito H AD - Laboratory of Molecular Pathology & Metabolic Disease, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Chiba, Japan. FAU - Goto, Akifumi AU - Goto A AD - Laboratory of Molecular Pathology & Metabolic Disease, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Chiba, Japan. FAU - Migitaka, Ryota AU - Migitaka R AD - Laboratory of Molecular Pathology & Metabolic Disease, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Chiba, Japan. FAU - Miura, Kumi AU - Miura K AD - Laboratory of Molecular Pathology & Metabolic Disease, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Chiba, Japan. FAU - Okita, Naoyuki AU - Okita N AD - Division of Pathological Biochemistry, Faculty of Pharmaceutical Sciences, Sanyo-Onoda City University, Sanyo-onoda, Yamaguchi, Japan. FAU - Sudo, Yuka AU - Sudo Y AD - Laboratory of Molecular Pathology & Metabolic Disease, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Chiba, Japan. FAU - Tagawa, Ryoma AU - Tagawa R AD - Laboratory of Molecular Pathology & Metabolic Disease, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Chiba, Japan. FAU - Yoshida, Miki AU - Yoshida M AD - Laboratory of Molecular Pathology & Metabolic Disease, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Chiba, Japan. FAU - Umemori, Ai AU - Umemori A AD - Laboratory of Molecular Pathology & Metabolic Disease, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Chiba, Japan. FAU - Nakagawa, Yoshimi AU - Nakagawa Y AD - Division of Complex Biosystem Research, Department of Research and Development, Institute of Natural Medicine, University of Toyama, Toyama, Japan; International Institute for Integrative Sleep Medicine (WPI-IIIS), University of Tsukuba, Ibaraki, Japan. FAU - Shimano, Hitoshi AU - Shimano H AD - Department of Internal Medicine (Endocrinology and Metabolism), Faculty of Medicine, University of Tsukuba, Tsukuba, Japan; International Institute for Integrative Sleep Medicine (WPI-IIIS), University of Tsukuba, Ibaraki, Japan; Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA), University of Tsukuba, Ibaraki, Japan; Japan Agency for Medical Research and Development-Core Research for Evolutional Science and Technology (AMED-CREST), Tokyo, Japan. FAU - Higami, Yoshikazu AU - Higami Y AD - Laboratory of Molecular Pathology & Metabolic Disease, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Chiba, Japan. Electronic address: higami@rs.tus.ac.jp. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20210817 PL - England TA - Exp Gerontol JT - Experimental gerontology JID - 0047061 SB - IM MH - Aging MH - Animals MH - *Caloric Restriction MH - Muscle Fibers, Skeletal/pathology MH - Muscle, Skeletal/pathology MH - Muscular Atrophy/pathology/prevention & control MH - *Quality of Life MH - Rats OTO - NOTNLM OT - Aging OT - Caloric restriction OT - Muscle fiber type OT - Skeletal muscle EDAT- 2021/08/21 06:00 MHDA- 2021/10/21 06:00 CRDT- 2021/08/20 20:14 PHST- 2021/03/16 00:00 [received] PHST- 2021/08/05 00:00 [revised] PHST- 2021/08/10 00:00 [accepted] PHST- 2021/08/21 06:00 [pubmed] PHST- 2021/10/21 06:00 [medline] PHST- 2021/08/20 20:14 [entrez] AID - S0531-5565(21)00301-6 [pii] AID - 10.1016/j.exger.2021.111519 [doi] PST - ppublish SO - Exp Gerontol. 2021 Oct 15;154:111519. doi: 10.1016/j.exger.2021.111519. Epub 2021 Aug 17.