PMID- 34417692
OWN - NLM
STAT- MEDLINE
DCOM- 20220120
LR  - 20220120
IS  - 1614-7499 (Electronic)
IS  - 0944-1344 (Linking)
VI  - 29
IP  - 4
DP  - 2022 Jan
TI  - Landfill soil leachates from Nigeria and India induced DNA damage and alterations 
      in genes associated with apoptosis in Jurkat cell.
PG  - 5256-5268
LID - 10.1007/s11356-021-15985-x [doi]
AB  - Landfill soil leachates, containing myriad of xenobiotics, increase genotoxic and 
      cytotoxic stress-induced cell death. However, the underlying mechanism involved 
      in the elimination of the damaged cells is yet to be fully elucidated. This study 
      investigated the apoptotic processes induced in lymphoma (Jurkat) cells by 
      landfill soil leachates from Olusosun (OSL, Nigeria) and Nagpur (NPL, India). 
      Jurkat was incubated with sub-lethal concentrations of OSL and NPL for 24 h and 
      analyzed for DNA fragmentation and apoptosis using agarose gel electrophoresis 
      and Hoechst 33258-PI staining, respectively. Complementary DNA expression 
      profiling of some pro-apoptotic and anti-apoptotic genes regulating apoptosis was 
      also analyzed using real-time PCR (RT-PCR) method. Agarose gel electrophoresis 
      revealed DNA fragmentations in OSL and NPL-treated cells. Hoecsht-33258 - 
      Propidium Iodide (PI) based apoptotic analysis confirmed apoptotic cell death in 
      exposed Jurkat. RT-PCR analysis revealed different fold changes in the pro- and 
      anti-apoptotic genes in OSL and NPL-treated Jurkat. There was significant 
      increase in fold change of the up-regulated genes; apoptosis inducing factor 
      mitochondrion-associated 2 (AIFM2), Fas-associated death domain (FADD), 
      Caspase-2, Caspase-6, BH3 interacting domain death agonist (BID), tumor 
      suppressor (p53), and BCL2 associated agonist of cell death (BAD) and 
      down-regulation of apoptosis inhibitor 5 (API5). Results suggest that OSL and NPL 
      elicited genotoxic stress-related apoptosis in Jurkat. The dysregulation in the 
      expression of genes involved in apoptotic processes in wildlife and human exposed 
      to landfill emissions may increase aetiology of various pathological diseases 
      including cancer.
CI  - (c) 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, 
      part of Springer Nature.
FAU - Alimba, Chibuisi G
AU  - Alimba CG
AUID- ORCID: 0000-0002-9393-1175
AD  - Cell Biology and Genetics Unit, Department of Zoology, University of Ibadan, 
      Ibadan, Nigeria. chivoptera@yahoo.com.
AD  - Department of Toxicology, Leibniz Research Centre for Working Environment and 
      Human Factors (IfADo), Technical University of Dortmund, 44139, Dortmund, 
      Germany. chivoptera@yahoo.com.
FAU - Rudrashetti, Ashwinkumar P
AU  - Rudrashetti AP
AD  - Environmental Biotechnology and Genomic Division, CSIR-National Environmental 
      Engineering Research Institute (NEERI), Nagpur, 440020, India.
FAU - Sivanesan, Saravanadevi
AU  - Sivanesan S
AD  - Health and Toxicity Cell (HTC), CSIR-National Environmental Engineering Research 
      Institute (NEERI), Nagpur, 440020, India.
AD  - Academy of Scientific, Innovative Research (AcSIR), Ghaziabad, U.P., India.
FAU - Krishnamurthi, Kannan
AU  - Krishnamurthi K
AD  - Health and Toxicity Cell (HTC), CSIR-National Environmental Engineering Research 
      Institute (NEERI), Nagpur, 440020, India. k_krishnamurthi@neeri.res.in.
AD  - Academy of Scientific, Innovative Research (AcSIR), Ghaziabad, U.P., India. 
      k_krishnamurthi@neeri.res.in.
LA  - eng
PT  - Journal Article
DEP - 20210821
PL  - Germany
TA  - Environ Sci Pollut Res Int
JT  - Environmental science and pollution research international
JID - 9441769
RN  - 0 (Soil)
RN  - 0 (Water Pollutants, Chemical)
RN  - EC 3.4.22.- (Caspases)
SB  - IM
MH  - Apoptosis
MH  - *Caspases
MH  - DNA Damage
MH  - Humans
MH  - Jurkat Cells
MH  - Nigeria
MH  - Soil
MH  - Waste Disposal Facilities
MH  - *Water Pollutants, Chemical
OTO - NOTNLM
OT  - Apoptosis
OT  - Genotoxicity
OT  - Landfill soil leachates
OT  - Lymphoma (Jurkat) cell
OT  - Real time-PCR analysis
EDAT- 2021/08/22 06:00
MHDA- 2022/01/21 06:00
CRDT- 2021/08/21 06:15
PHST- 2021/06/04 00:00 [received]
PHST- 2021/08/11 00:00 [accepted]
PHST- 2021/08/22 06:00 [pubmed]
PHST- 2022/01/21 06:00 [medline]
PHST- 2021/08/21 06:15 [entrez]
AID - 10.1007/s11356-021-15985-x [pii]
AID - 10.1007/s11356-021-15985-x [doi]
PST - ppublish
SO  - Environ Sci Pollut Res Int. 2022 Jan;29(4):5256-5268. doi: 
      10.1007/s11356-021-15985-x. Epub 2021 Aug 21.