PMID- 34419775
OWN - NLM
STAT- MEDLINE
DCOM- 20211104
LR  - 20211104
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 261
DP  - 2021 Oct
TI  - Development and in vivo evaluation of MGF100-1R deletion mutant in an African 
      swine fever virus Chinese strain.
PG  - 109208
LID - S0378-1135(21)00231-5 [pii]
LID - 10.1016/j.vetmic.2021.109208 [doi]
AB  - African swine fever virus (ASFV) is a large nucleoplasmic DNA virus, in which the 
      genome is around 170-198 kilobases (kb). More than 50 % genes have unknown 
      functions. Here, MGF100-1R gene is chosen to study the primary function and 
      sublocalization. The gene was located at the left variable region of the ASFV 
      genome that belongs to MGF100 families. It located at the cytoplasm without 
      cytotoxic activities. However, it related to induce the transcriptional levels of 
      pro-inflammatory cytokines. A deletion mutant of MGF100-1R gene was constructed 
      based on ASFV Chinese strain GZ201801. The recombinant deletion mutant 
      (ASFV big up tri, openMGF100-1R) was demonstrated in vitro that the gene is non-essential for 
      virus replication with a similar replication kinetics in bone marrow-derived 
      macrophages (BMDMs) cell cultures when compared to parental virus. In vivo 
      evaluation, ASFV big up tri, openMGF100-1R was inoculated intramuscularly and led to a similar 
      pathogenesis that caused by the parental ASFV GZ201801, confirming that deletion 
      of MGF100-1R gene from the ASFV genome does not impact virulence.
CI  - Copyright (c) 2021. Published by Elsevier B.V.
FAU - Liu, Yingnan
AU  - Liu Y
AD  - Shanghai Veterinary Research Institute, CAAS, Shanghai, 200241, China.
FAU - Li, Yao
AU  - Li Y
AD  - Shanghai Veterinary Research Institute, CAAS, Shanghai, 200241, China.
FAU - Xie, Zhenhua
AU  - Xie Z
AD  - Shanghai Veterinary Research Institute, CAAS, Shanghai, 200241, China.
FAU - Ao, Qingying
AU  - Ao Q
AD  - Shanghai Veterinary Research Institute, CAAS, Shanghai, 200241, China.
FAU - Di, Dongdong
AU  - Di D
AD  - The Spirit Jinyu Biological Pharmaceutical Co. Ltd, Inner Mongolia, 010030, 
      China.
FAU - Yu, Wanqi
AU  - Yu W
AD  - Shanghai Veterinary Research Institute, CAAS, Shanghai, 200241, China.
FAU - Lv, Lu
AU  - Lv L
AD  - Shanghai Veterinary Research Institute, CAAS, Shanghai, 200241, China.
FAU - Zhong, Qiuping
AU  - Zhong Q
AD  - Shanghai Veterinary Research Institute, CAAS, Shanghai, 200241, China.
FAU - Song, Yingying
AU  - Song Y
AD  - Shanghai Veterinary Research Institute, CAAS, Shanghai, 200241, China.
FAU - Liao, Xinxin
AU  - Liao X
AD  - Shanghai Veterinary Research Institute, CAAS, Shanghai, 200241, China.
FAU - Song, Qingqing
AU  - Song Q
AD  - The Spirit Jinyu Biological Pharmaceutical Co. Ltd, Inner Mongolia, 010030, 
      China.
FAU - Wang, Heng
AU  - Wang H
AD  - South China Agricultural University, Guangdong, 510642, China. Electronic 
      address: wangheng@scau.edu.cn.
FAU - Chen, Hongjun
AU  - Chen H
AD  - Shanghai Veterinary Research Institute, CAAS, Shanghai, 200241, China. Electronic 
      address: vetchj@shvri.ac.cn.
LA  - eng
PT  - Journal Article
DEP - 20210814
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
SB  - IM
MH  - African Swine Fever/*virology
MH  - African Swine Fever Virus/*genetics/*pathogenicity
MH  - Animals
MH  - Cells, Cultured
MH  - China
MH  - Genes, Viral/genetics
MH  - Macrophages/virology
MH  - Sequence Deletion/*genetics
MH  - Swine
MH  - Virulence/genetics
MH  - Virus Replication/genetics
OTO - NOTNLM
OT  - ASFV
OT  - African swine fever virus
OT  - Deletion mutant
OT  - MGF100-1R
OT  - Pathogenicity
EDAT- 2021/08/23 06:00
MHDA- 2021/11/05 06:00
CRDT- 2021/08/22 20:54
PHST- 2021/05/10 00:00 [received]
PHST- 2021/08/11 00:00 [accepted]
PHST- 2021/08/23 06:00 [pubmed]
PHST- 2021/11/05 06:00 [medline]
PHST- 2021/08/22 20:54 [entrez]
AID - S0378-1135(21)00231-5 [pii]
AID - 10.1016/j.vetmic.2021.109208 [doi]
PST - ppublish
SO  - Vet Microbiol. 2021 Oct;261:109208. doi: 10.1016/j.vetmic.2021.109208. Epub 2021 
      Aug 14.