PMID- 34419855
OWN - NLM
STAT- MEDLINE
DCOM- 20220126
LR  - 20220126
IS  - 1532-8198 (Electronic)
IS  - 1092-9134 (Linking)
VI  - 54
DP  - 2021 Oct
TI  - The role of alpha-smooth muscle actin in confirming the microinvasion of laryngeal 
      squamous cell carcinoma.
PG  - 151804
LID - S1092-9134(21)00104-0 [pii]
LID - 10.1016/j.anndiagpath.2021.151804 [doi]
AB  - BACKGROUND: The diagnosis of microinvasive laryngeal squamous cell carcinoma 
      (LSCC) is not always straightforward and sometimes can be very challenge in daily 
      clinical practice. The focus lies in the confirmation of microinvasion. 
      Cancer-associated fibroblasts (CAFs), as the major element of reactive tumor 
      stroma, are believed to participate actively in the growth and invasion of tumor 
      cells. OBJECTIVES: To evaluate the diagnostic role of alpha-smooth muscle actin 
      (alpha-SMA) labelling CAFs in microinvasive LSCC. METHODS: A total of 81 laryngeal 
      biopsy specimens were retrieved, including 41 cases of microinvasive LSCC with 
      depth of invasion no more than 3 mm, 20 laryngeal squamous intraepithelial lesion 
      (SIL), and 20 benign pseudoepitheliomatous hyperplasia (PEH). All cases were 
      stained for immunohistochemistry, using antibody against the alpha-SMA antigen. The 
      correlation between the presence of CAFs in microinvasive LSCC and tumor 
      histological characteristics was investigated. RESULTS: Immunoreactivity of alpha-SMA 
      was detected in twenty-nine microinvasive LSCC (29/41, 71%), while no reactivity 
      was observed in laryngeal SIL (0/20, 0%), and rarely in PEH (2/20, 10%). The 
      alpha-SMA expression pattern in stroma of microinvasive LSCC was significantly 
      different from that of SIL (chi(2) = 26.966, p = 0.000) and PEH (chi(2) = 19.838, 
      p = 0.000). In addition, there seemed to be a certain correlation between the 
      histological characteristics of microinvasive LSCC and the presence of 
      interstitial CAFs. CONCLUSIONS: This study highlights the practical role of 
      utilizing alpha-SMA in the pathological diagnosis of microinvasive LSCC, with 
      emphasis on variable histomorphologic features of microinvasive LSCC.
CI  - Copyright (c) 2021. Published by Elsevier Inc.
FAU - Bai, Yuping
AU  - Bai Y
AD  - Department of Pathology, Beijing Tongren Hospital, Capital Medical University, 
      Beijing Key Laboratory of Head and Neck Molecular Diagnostic Pathology, Beijing 
      100730, China.
FAU - Yue, Changli
AU  - Yue C
AD  - Department of Pathology, Beijing Tongren Hospital, Capital Medical University, 
      Beijing Key Laboratory of Head and Neck Molecular Diagnostic Pathology, Beijing 
      100730, China.
FAU - Lu, Zhichun
AU  - Lu Z
AD  - Department of Pathology and Laboratory Medicine, Boston Medical Center/Boston 
      University School of Medicine, Boston, MA, United States.
FAU - Li, Pingdong
AU  - Li P
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, Beijing Tongren 
      Hospital, Capital Medical University, Beijing 100730, China.
FAU - Liu, Honggang
AU  - Liu H
AD  - Department of Pathology, Beijing Tongren Hospital, Capital Medical University, 
      Beijing Key Laboratory of Head and Neck Molecular Diagnostic Pathology, Beijing 
      100730, China. Electronic address: liuhg1125@163.com.
LA  - eng
PT  - Journal Article
DEP - 20210804
PL  - United States
TA  - Ann Diagn Pathol
JT  - Annals of diagnostic pathology
JID - 9800503
RN  - 0 (Actins)
RN  - 0 (Biomarkers, Tumor)
SB  - IM
MH  - Actins/metabolism
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Biomarkers, Tumor/metabolism
MH  - Carcinoma, Squamous Cell/diagnosis/*pathology
MH  - Female
MH  - Head and Neck Neoplasms/diagnosis/*pathology
MH  - Humans
MH  - Immunohistochemistry/methods
MH  - Laryngeal Neoplasms/diagnosis/*pathology
MH  - Male
MH  - Middle Aged
MH  - Muscle, Smooth/metabolism/*pathology
MH  - Squamous Cell Carcinoma of Head and Neck/metabolism/pathology
OTO - NOTNLM
OT  - Cancer-associated fibroblasts
OT  - Histomorphologic feature
OT  - Microinvasive laryngeal squamous cell carcinoma
OT  - Pathological diagnosis
OT  - alpha-Smooth muscle actin
EDAT- 2021/08/23 06:00
MHDA- 2022/01/27 06:00
CRDT- 2021/08/22 20:58
PHST- 2021/05/14 00:00 [received]
PHST- 2021/07/06 00:00 [revised]
PHST- 2021/07/25 00:00 [accepted]
PHST- 2021/08/23 06:00 [pubmed]
PHST- 2022/01/27 06:00 [medline]
PHST- 2021/08/22 20:58 [entrez]
AID - S1092-9134(21)00104-0 [pii]
AID - 10.1016/j.anndiagpath.2021.151804 [doi]
PST - ppublish
SO  - Ann Diagn Pathol. 2021 Oct;54:151804. doi: 10.1016/j.anndiagpath.2021.151804. 
      Epub 2021 Aug 4.