PMID- 34420159 OWN - NLM STAT- MEDLINE DCOM- 20230110 LR - 20230127 IS - 2629-3277 (Electronic) IS - 2629-3277 (Linking) VI - 19 IP - 1 DP - 2023 Jan TI - Combining Bone Collagen Matrix with hUC-MSCs for Application to Alveolar Process Cleft in a Rabbit Model. PG - 133-154 LID - 10.1007/s12015-021-10221-y [doi] AB - BACKGROUND: Most materials used clinically for filling severe bone defects either cannot induce bone re-generation or exhibit low bone conversion, therefore, their therapeutic effects are limited. Human umbilical cord mesenchymal stem cells (hUC-MSCs) exhibit good osteoinduction. However, the mechanism by which combining a heterogeneous bone collagen matrix with hUC-MSCs to repair the bone defects of alveolar process clefts remains unclear. METHODS: A rabbit alveolar process cleft model was established by removing the bone tissue from the left maxillary bone. Forty-eight young Japanese white rabbits (JWRs) were divided into normal, control, material and MSCs groups. An equal volume of a bone collagen matrix alone or combined with hUC-MSCs was implanted in the defect. X-ray, micro-focus computerized tomography (micro-CT), blood analysis, histochemical staining and TUNEL were used to detect the newly formed bone in the defect area at 3 and 6 months after the surgery. RESULTS: The bone formation rate obtained from the skull tissue in MSCs group was significantly higher than that in control group at 3 months (P < 0.01) and 6 months (P < 0.05) after the surgery. The apoptosis rate in the MSCs group was significantly higher at 3 months after the surgery (P < 0.05) and lower at 6 months after the surgery (P < 0.01) than those in the normal group. CONCLUSIONS: Combining bone collagen matrix with hUC-MSCs promoted the new bone regeneration in the rabbit alveolar process cleft model through promoting osteoblasts formations and chondrocyte growth, and inducing type I collagen formation and BMP-2 generation. CI - (c) 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature. FAU - Sun, Xue-Cheng AU - Sun XC AUID- ORCID: 0000-0002-1689-2627 AD - Reproductive and Genetic Center of National Research Institute for Family Planning, Beijing, 100081, China. AD - Medical Genetics, Zibo Maternal and Child Health Hospital, Zibo, 255000, China. FAU - Wang, Hu AU - Wang H AD - Reproductive and Genetic Center of National Research Institute for Family Planning, Beijing, 100081, China. AD - Graduate Schools, Peking Union Medical College, Beijing, 100730, China. FAU - Zhang, Dan AU - Zhang D AD - Reproductive and Genetic Center of National Research Institute for Family Planning, Beijing, 100081, China. AD - Graduate Schools, Peking Union Medical College, Beijing, 100730, China. FAU - Li, Jian-Hui AU - Li JH AD - Reproductive and Genetic Center of National Research Institute for Family Planning, Beijing, 100081, China. AD - Graduate Schools, Peking Union Medical College, Beijing, 100730, China. FAU - Yin, Li-Qiang AU - Yin LQ AD - Yantai Zhenghai Bio-Tech Co., Ltd., Shandong, 264006, China. FAU - Yan, Yu-Fang AU - Yan YF AD - Yantai Zhenghai Bio-Tech Co., Ltd., Shandong, 264006, China. FAU - Ma, Xu AU - Ma X AD - Reproductive and Genetic Center of National Research Institute for Family Planning, Beijing, 100081, China. hongfeixia@126.com. AD - Graduate Schools, Peking Union Medical College, Beijing, 100730, China. hongfeixia@126.com. AD - Genetic Center of National Research Institute for Family Planning, Beijing, 100081, China. hongfeixia@126.com. FAU - Xia, Hong-Fei AU - Xia HF AD - Reproductive and Genetic Center of National Research Institute for Family Planning, Beijing, 100081, China. gentic@263.net.cn. AD - Graduate Schools, Peking Union Medical College, Beijing, 100730, China. gentic@263.net.cn. AD - Genetic Center of National Research Institute for Family Planning, Beijing, 100081, China. gentic@263.net.cn. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20210822 PL - United States TA - Stem Cell Rev Rep JT - Stem cell reviews and reports JID - 101752767 RN - 9007-34-5 (Collagen) SB - IM MH - Animals MH - Rabbits MH - Humans MH - *Collagen MH - Osteogenesis MH - Bone Regeneration MH - *Mesenchymal Stem Cells MH - Alveolar Process OTO - NOTNLM OT - Alveolar process cleft OT - Bone collagen matrix OT - Micro-CT OT - Young rabbit OT - hUC-MSCs EDAT- 2021/08/23 06:00 MHDA- 2023/01/11 06:00 CRDT- 2021/08/22 21:09 PHST- 2021/07/12 00:00 [accepted] PHST- 2021/08/23 06:00 [pubmed] PHST- 2023/01/11 06:00 [medline] PHST- 2021/08/22 21:09 [entrez] AID - 10.1007/s12015-021-10221-y [pii] AID - 10.1007/s12015-021-10221-y [doi] PST - ppublish SO - Stem Cell Rev Rep. 2023 Jan;19(1):133-154. doi: 10.1007/s12015-021-10221-y. Epub 2021 Aug 22.