PMID- 34423029
OWN - NLM
STAT- MEDLINE
DCOM- 20210929
LR  - 20210929
IS  - 2314-6141 (Electronic)
IS  - 2314-6133 (Print)
VI  - 2021
DP  - 2021
TI  - circGLI3 Inhibits Oxidative Stress by Regulating the miR-339-5p/VEGFA Axis in 
      IPEC-J2 Cells.
PG  - 1086206
LID - 10.1155/2021/1086206 [doi]
LID - 1086206
AB  - As a new type of noncoding RNA, circular RNA (circRNA) is stable in cells and not 
      easily degraded. This type of RNA can also competitively bind miRNAs to regulate 
      the expression of their target genes. The role of circRNA in the mechanism of 
      intestinal oxidative stress (OS) in weaned piglets is still unclear. In our 
      research, diquat (DQ) was used to induce OS in small intestinal epithelial cells 
      (IPEC-J2) to construct an OS cell model. Mechanistically, dual luciferase 
      reporter assays, fluorescence in situ hybridization (FISH), and western blotting 
      were performed to confirm that circGLI3 directly sponged miR-339-5p and regulated 
      the expression of VEGFA. Overexpression of circGLI3 promoted IPEC-J2 cell 
      proliferation, increased the proportion of S-phase cells (P < 0.01), and reduced 
      reactive oxygen species (ROS) generation when IPEC-J2 cells were subjected to OS. 
      circGLI3 can increase the activity of glutathione peroxidase (GSH-Px) and the 
      total antioxidant capacity (T-AOC) in IPEC-J2 cells and reduce the 
      malondialdehyde (MDA) content and levels of inflammatory factors. Therefore, 
      overexpression of circGLI3 reduced oxidative damage, whereas miR-339-5p mimic 
      counteracted these effects. We identified a regulatory network composed of 
      circGLI3, miR-339-5p, and VEGFA and verified that circGLI3 regulates VEGFA by 
      directly binding miR-339-5p. The expression of VEGFA affects IPEC-J2 cell 
      proliferation, cell cycle progression, and ROS content and changes the levels of 
      antioxidant enzymes and inflammatory factors. This study reveals the molecular 
      mechanism by which circGLI3 inhibits OS in the intestine of piglets and provides 
      a theoretical basis for further research on the effect of OS on intestinal 
      function.
CI  - Copyright (c) 2021 Zhi-xin Li et al.
FAU - Li, Zhi-Xin
AU  - Li ZX
AUID- ORCID: 0000-0002-0218-1680
AD  - Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control 
      and Prevention, College of Animal Science and Technology, Shandong Agricultural 
      University, No. 61 Daizong Street, Tai'an City, Shandong Province, 271018, China.
FAU - Wang, Li-Xue
AU  - Wang LX
AUID- ORCID: 0000-0001-9704-1527
AD  - Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control 
      and Prevention, College of Animal Science and Technology, Shandong Agricultural 
      University, No. 61 Daizong Street, Tai'an City, Shandong Province, 271018, China.
FAU - Zhang, Yu
AU  - Zhang Y
AUID- ORCID: 0000-0002-4326-6031
AD  - Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control 
      and Prevention, College of Animal Science and Technology, Shandong Agricultural 
      University, No. 61 Daizong Street, Tai'an City, Shandong Province, 271018, China.
FAU - Chen, Wei
AU  - Chen W
AUID- ORCID: 0000-0002-2901-5156
AD  - Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control 
      and Prevention, College of Animal Science and Technology, Shandong Agricultural 
      University, No. 61 Daizong Street, Tai'an City, Shandong Province, 271018, China.
FAU - Zeng, Yong-Qing
AU  - Zeng YQ
AUID- ORCID: 0000-0002-0740-2470
AD  - Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control 
      and Prevention, College of Animal Science and Technology, Shandong Agricultural 
      University, No. 61 Daizong Street, Tai'an City, Shandong Province, 271018, China.
LA  - eng
PT  - Journal Article
DEP - 20210811
PL  - United States
TA  - Biomed Res Int
JT  - BioMed research international
JID - 101600173
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Circular)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - A9A615U4MP (Diquat)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
SB  - IM
MH  - Animals
MH  - Cell Cycle/drug effects
MH  - Cell Line
MH  - Cell Proliferation/drug effects
MH  - Diquat/*adverse effects
MH  - Epithelial Cells/chemistry/cytology/drug effects
MH  - Gene Expression Regulation/drug effects
MH  - Glutathione Peroxidase/metabolism
MH  - Intestine, Small/chemistry/*cytology/drug effects
MH  - Malondialdehyde/metabolism
MH  - MicroRNAs/*genetics
MH  - Models, Biological
MH  - Oxidative Stress
MH  - RNA, Circular/*genetics
MH  - Swine
MH  - Vascular Endothelial Growth Factor A/*genetics/metabolism
PMC - PMC8376464
COIS- The authors declare that they have no competing interests.
EDAT- 2021/08/24 06:00
MHDA- 2021/09/30 06:00
PMCR- 2021/08/11
CRDT- 2021/08/23 06:38
PHST- 2021/05/18 00:00 [received]
PHST- 2021/07/10 00:00 [revised]
PHST- 2021/07/26 00:00 [accepted]
PHST- 2021/08/23 06:38 [entrez]
PHST- 2021/08/24 06:00 [pubmed]
PHST- 2021/09/30 06:00 [medline]
PHST- 2021/08/11 00:00 [pmc-release]
AID - 10.1155/2021/1086206 [doi]
PST - epublish
SO  - Biomed Res Int. 2021 Aug 11;2021:1086206. doi: 10.1155/2021/1086206. eCollection 
      2021.