PMID- 34424160
OWN - NLM
STAT- MEDLINE
DCOM- 20210906
LR  - 20211006
IS  - 1465-2099 (Electronic)
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 102
IP  - 8
DP  - 2021 Aug
TI  - A virulent and pathogenic infectious clone of Senecavirus A.
LID - 10.1099/jgv.0.001643 [doi]
LID - 001643
AB  - Senecavirus A (SVA) is a picornavirus that circulates in swine populations 
      worldwide causing vesicular disease (VD) in affected animals. Here we developed a 
      reverse genetics system for SVA based on the well-characterized wild-type SVA 
      strain SD15-26 (wt SVA SD15-26). The full-length cDNA genome of SVA was cloned 
      into a plasmid under a T7 RNA polymerase promoter. Following in vitro 
      transcription, the genomic viral RNA was transfected into BHK-21 cells and rescue 
      of infectious virus (rSVA SD15-26) was shown by inoculation of highly susceptible 
      H1299 cells. In vitro characterization of the rSVA SD15-26 showed similar 
      replication properties and protein expression levels as the wt SVA SD15-26. A 
      pathogenesis study was conducted in 15-week-old finishing pigs to evaluate the 
      pathogenicity and infection dynamics of the rSVA SD15-26 virus in comparison to 
      the wt SVA SD15-26. Animals from both rSVA- and wt SVA SD15-26-inoculated groups 
      presented characteristic SVA clinical signs (lethargy and lameness) followed by 
      the development of vesicular lesions on the snout and/or feet. The clinical 
      outcome of infection, including disease onset, severity and duration was similar 
      in rSVA- and the wt SVA SD15-26-inoculated animals. All animals inoculated with 
      rSVA or with wt SVA SD15-26 presented a short-term viremia, and animals from both 
      groups shed similar amounts of virus in oral and nasal secretion, and faeces. Our 
      data demonstrates that the rSVA SD5-26 clone is fully virulent and pathogenic in 
      pigs, presenting comparable pathogenesis and infection dynamics to the wt SVA 
      SD15-26 strain. The infectious clone generated here is a useful platform to study 
      virulence determinants of SVA, and to dissect other aspects of SVA infection 
      biology, pathogenesis and persistence.
FAU - Fernandes, Maureen H V
AU  - Fernandes MHV
AD  - Department of Population Medicine and Diagnostic Sciences, Animal Health 
      Diagnostic Center, College of Veterinary Medicine, Cornell University, Ithaca, 
      NY, USA.
AD  - Department of Veterinary and Biomedical Sciences, South Dakota State University, 
      Brookings, SD, USA.
FAU - de Lima, Marcelo
AU  - de Lima M
AD  - Department of Veterinary and Biomedical Sciences, South Dakota State University, 
      Brookings, SD, USA.
AD  - Laboratorio de Virologia e Imunologia, Faculdade de Veterinaria, Universidade 
      Federal de Pelotas, Pelotas, RS, Brazil.
FAU - Joshi, Lok R
AU  - Joshi LR
AD  - Department of Population Medicine and Diagnostic Sciences, Animal Health 
      Diagnostic Center, College of Veterinary Medicine, Cornell University, Ithaca, 
      NY, USA.
AD  - Department of Veterinary and Biomedical Sciences, South Dakota State University, 
      Brookings, SD, USA.
FAU - Diel, Diego G
AU  - Diel DG
AD  - Department of Population Medicine and Diagnostic Sciences, Animal Health 
      Diagnostic Center, College of Veterinary Medicine, Cornell University, Ithaca, 
      NY, USA.
AD  - Department of Veterinary and Biomedical Sciences, South Dakota State University, 
      Brookings, SD, USA.
LA  - eng
GR  - 2019-67015-29830/National Institute of Food and Agriculture/
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - Senecavirus A
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Cricetinae
MH  - Humans
MH  - Picornaviridae/*pathogenicity
MH  - *Picornaviridae Infections/veterinary/virology
MH  - Swine
MH  - Swine Diseases/*virology
MH  - Viremia/virology
MH  - Virulence
PMC - PMC8489587
OTO - NOTNLM
OT  - SVA
OT  - SVV
OT  - Seneca Valley virus
OT  - infectious clone
OT  - recombinant
OT  - reverse genetics
OT  - vesicular disease
COIS- The authors declare that there are no conflicts of interest.
EDAT- 2021/08/24 06:00
MHDA- 2021/09/07 06:00
PMCR- 2021/08/23
CRDT- 2021/08/23 12:19
PHST- 2021/08/23 12:19 [entrez]
PHST- 2021/08/24 06:00 [pubmed]
PHST- 2021/09/07 06:00 [medline]
PHST- 2021/08/23 00:00 [pmc-release]
AID - 001643 [pii]
AID - 10.1099/jgv.0.001643 [doi]
PST - ppublish
SO  - J Gen Virol. 2021 Aug;102(8):001643. doi: 10.1099/jgv.0.001643.