PMID- 34424397 OWN - NLM STAT- MEDLINE DCOM- 20220210 LR - 20220210 IS - 1432-0878 (Electronic) IS - 0302-766X (Linking) VI - 386 IP - 2 DP - 2021 Nov TI - Decellularization of kidney tissue: comparison of sodium lauryl ether sulfate and sodium dodecyl sulfate for allotransplantation in rat. PG - 365-378 LID - 10.1007/s00441-021-03517-5 [doi] AB - An automatic decellularization device was developed to perfuse and decellularize male rats' kidneys using both sodium lauryl ether sulfate (SLES) and sodium dodecyl sulfate (SDS) and to compare their efficacy in kidney decellularization and post-transplantation angiogenesis. Kidneys were perfused with either 1% SDS solution for 4 h or 1% SLES solution for 6 h. The decellularized scaffolds were stained with hematoxylin and eosin, periodic acid Schiff, Masson's trichrome, and Alcian blue to determine cell removal and glycogen, collagen, and glycosaminoglycan contents, respectively. Moreover, scanning electron microscopy was performed to evaluate the cell removal and preservation of microarchitecture of both SDS and SLES scaffolds. Additionally, DNA quantification assay was applied for all groups in order to measure residual DNA in the scaffolds and normal kidney. In order to demonstrate biocompatibility of the decellularized scaffolds, human umbilical cord mesenchymal stromal/stem cells (hUC-MSCs) were seeded on the scaffolds. In addition, the allotransplantation was performed in back muscle and angiogenesis was evaluated. Complete cell removal in both SLES and SDS groups was observed in scanning electron microscopy and DNA quantification assays. Moreover, the extracellular matrix (ECM) architecture of rat kidney in the SLES group was significantly preserved better than the SDS group. The hUC-MSCs were successfully migrated from the cell culture plate surface into the SDS and SLES decellularized scaffolds. The formation of blood vessels was observed in the kidney in both SLES and SDS decellularized kidneys. The better preservation of ECM than SDS introduces SLES as the solvent of choice for kidney decellularization. CI - (c) 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. FAU - Keshvari, Mohammad Amin AU - Keshvari MA AD - The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran. FAU - Afshar, Alireza AU - Afshar A AD - The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran. AD - Percia Vista Biotechnology Company, Bushehr, Iran. FAU - Daneshi, Sajad AU - Daneshi S AD - Department of Anatomical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran. FAU - Khoradmehr, Arezoo AU - Khoradmehr A AD - The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran. FAU - Baghban, Mandana AU - Baghban M AD - Department of Obstetrics and Gynecology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. FAU - Muhaddesi, Mahdi AU - Muhaddesi M AD - The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran. FAU - Behrouzi, Pouya AU - Behrouzi P AD - The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran. FAU - Miri, Mohammad Reza AU - Miri MR AD - The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran. FAU - Azari, Hossein AU - Azari H AD - The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran. FAU - Nabipour, Iraj AU - Nabipour I AD - The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran. FAU - Shirazi, Reza AU - Shirazi R AD - Department of Anatomy, School of Medical Sciences, Medicine & Health, UNSW Sydney, Sydney, Australia. FAU - Mahmudpour, Mehdi AU - Mahmudpour M AD - The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran. mehdimpr@gmail.com. FAU - Tamadon, Amin AU - Tamadon A AUID- ORCID: 0000-0002-0222-3035 AD - The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran. amintamaddon@yahoo.com. LA - eng GR - 1279/Bushehr University of Medical Sciences/ PT - Journal Article DEP - 20210823 PL - Germany TA - Cell Tissue Res JT - Cell and tissue research JID - 0417625 RN - 0 (Decellularized Extracellular Matrix) RN - 26183-44-8 (dodecyl polyoxyethylene sulfuric acid) RN - 368GB5141J (Sodium Dodecyl Sulfate) RN - 3WJQ0SDW1A (Polyethylene Glycols) SB - IM MH - Animals MH - Decellularized Extracellular Matrix/*chemistry MH - Kidney/*chemistry/cytology/ultrastructure MH - Polyethylene Glycols/*chemistry MH - Rats MH - Rats, Sprague-Dawley MH - Sodium Dodecyl Sulfate/*chemistry MH - Stem Cell Transplantation MH - Stem Cells/cytology MH - Tissue Engineering MH - Tissue Scaffolds/*chemistry OTO - NOTNLM OT - Decellularized scaffold OT - Kidney OT - Rat OT - Sodium dodecyl sulfate OT - Sodium lauryl ether sulfate EDAT- 2021/08/24 06:00 MHDA- 2022/02/11 06:00 CRDT- 2021/08/23 12:28 PHST- 2021/03/07 00:00 [received] PHST- 2021/08/04 00:00 [accepted] PHST- 2021/08/24 06:00 [pubmed] PHST- 2022/02/11 06:00 [medline] PHST- 2021/08/23 12:28 [entrez] AID - 10.1007/s00441-021-03517-5 [pii] AID - 10.1007/s00441-021-03517-5 [doi] PST - ppublish SO - Cell Tissue Res. 2021 Nov;386(2):365-378. doi: 10.1007/s00441-021-03517-5. Epub 2021 Aug 23.