PMID- 34428999
OWN - NLM
STAT- MEDLINE
DCOM- 20220913
LR  - 20220913
IS  - 1556-9535 (Electronic)
IS  - 1556-9527 (Linking)
VI  - 41
IP  - 3
DP  - 2022 Sep
TI  - Inhibition of hypoxia-inducible factor-1 by salidroside in an in vitro model of 
      choroidal neovascularization.
PG  - 203-209
LID - 10.1080/15569527.2021.1973023 [doi]
AB  - PURPOSE: As a characteristic of age-related macular degeneration (AMD), choroidal 
      neovascularization (CNV) causes severe vision loss. The current treatment has 
      limited efficacy. This study was to investigate effects of Salidroside against 
      CNV and explore its underlying mechanisms. METHODS: RF/6A cells were treated with 
      200 mM cobalt chloride (CoCl(2)) for 6 hr to mimic hypoxic condition. Cells were 
      then treated with Salidroside at 10, 30, and 100 microM for 24 hr. Cells treated with 
      DMSO were used as negative control. The cell proliferation was assessed using 
      3-(4,5-dimethylthiazol)-2,5-diphenyltetrazolium-bromid assay. The tube formation 
      was investigated on Matrigel. The cell migration was measured by a Transwell 
      assay. RT-qPCR was used to detect the gene expression. Immuohistochemistry and 
      western blot were used to detect the expression of proteins. RESULTS: Salidroside 
      significantly inhibited the cell migration and tube formation activity of RF/6A 
      cells under hypoxia. Moreover, Salidroside reduced the expression levels of 
      vascular endothelial growth factor (VEGF) and hypoxia-inducible factor-1 (HIF-1) 
      in RF/6A cells. CONCLUSIONS: Our data suggested that Salidroside could be a 
      potential novel therapeutic agent against CNV.
FAU - Yang, Haitao
AU  - Yang H
AD  - Department of Neurosurgery, The second Fuzhou Hospital Affiliated to Xiamen 
      University, Fuzhou, China.
FAU - Yang, Qingwu
AU  - Yang Q
AD  - Department of Neurosurgery, The second Fuzhou Hospital Affiliated to Xiamen 
      University, Fuzhou, China.
FAU - Zheng, Linfei
AU  - Zheng L
AD  - Department of Neurosurgery, The second Fuzhou Hospital Affiliated to Xiamen 
      University, Fuzhou, China.
LA  - eng
PT  - Journal Article
DEP - 20220715
PL  - England
TA  - Cutan Ocul Toxicol
JT  - Cutaneous and ocular toxicology
JID - 101266892
RN  - 0 (Glucosides)
RN  - 0 (Hypoxia-Inducible Factor 1)
RN  - 0 (Phenols)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - M983H6N1S9 (rhodioloside)
SB  - IM
MH  - *Choroidal Neovascularization/drug therapy
MH  - Glucosides
MH  - Humans
MH  - Hypoxia/complications/metabolism
MH  - Hypoxia-Inducible Factor 1
MH  - Phenols
MH  - Vascular Endothelial Growth Factor A/genetics/metabolism
OTO - NOTNLM
OT  - RF/6A cells
OT  - Salidroside
OT  - choroidal neovascularization
OT  - hypoxia-inducible factor-1
OT  - tube formation
EDAT- 2021/08/26 06:00
MHDA- 2022/09/14 06:00
CRDT- 2021/08/25 05:29
PHST- 2021/08/26 06:00 [pubmed]
PHST- 2022/09/14 06:00 [medline]
PHST- 2021/08/25 05:29 [entrez]
AID - 10.1080/15569527.2021.1973023 [doi]
PST - ppublish
SO  - Cutan Ocul Toxicol. 2022 Sep;41(3):203-209. doi: 10.1080/15569527.2021.1973023. 
      Epub 2022 Jul 15.