PMID- 34433575
OWN - NLM
STAT- MEDLINE
DCOM- 20211020
LR  - 20211102
IS  - 2373-2822 (Electronic)
IS  - 2373-2822 (Linking)
VI  - 8
IP  - 5
DP  - 2021 Sep-Oct
TI  - TMEM16A and TMEM16B Modulate Pheromone-Evoked Action Potential Firing in Mouse 
      Vomeronasal Sensory Neurons.
LID - ENEURO.0179-21.2021 [pii]
LID - 10.1523/ENEURO.0179-21.2021 [doi]
AB  - The mouse vomeronasal system controls several social behaviors. Pheromones and 
      other social cues are detected by sensory neurons in the vomeronasal organ (VNO). 
      Stimuli activate a transduction cascade that leads to membrane potential 
      depolarization, increase in cytosolic Ca(2+) level, and increased firing. The 
      Ca(2+)-activated chloride channels TMEM16A and TMEM16B are co-expressed within 
      microvilli of vomeronasal neurons, but their physiological role remains elusive. 
      Here, we investigate the contribution of each of these channels to vomeronasal 
      neuron firing activity by comparing wild-type (WT) and knock-out (KO) mice. 
      Performing loose-patch recordings from neurons in acute VNO slices, we show that 
      spontaneous activity is modified by Tmem16a KO, indicating that TMEM16A, but not 
      TMEM16B, is active under basal conditions. Upon exposure to diluted urine, a rich 
      source of mouse pheromones, we observe significant changes in activity. 
      Vomeronasal sensory neurons (VSNs) from Tmem16a cKO and Tmem16b KO mice show 
      shorter interspike intervals (ISIs) compared with WT mice, indicating that both 
      TMEM16A and TMEM16B modulate the firing pattern of pheromone-evoked activity in 
      VSNs.
CI  - Copyright (c) 2021 Hernandez-Clavijo et al.
FAU - Hernandez-Clavijo, Andres
AU  - Hernandez-Clavijo A
AUID- ORCID: 0000-0002-4122-3521
AD  - Neurobiology Group, SISSA, International School for Advanced Studies, Trieste 
      34136, Italy.
FAU - Sarno, Nicole
AU  - Sarno N
AD  - Neurobiology Group, SISSA, International School for Advanced Studies, Trieste 
      34136, Italy.
FAU - Gonzalez-Velandia, Kevin Y
AU  - Gonzalez-Velandia KY
AD  - Neurobiology Group, SISSA, International School for Advanced Studies, Trieste 
      34136, Italy.
FAU - Degen, Rudolf
AU  - Degen R
AUID- ORCID: 0000-0002-8513-9030
AD  - Department of Chemosensation, Institute for Biology II, RWTH Aachen University, 
      Aachen D-52074, Germany.
FAU - Fleck, David
AU  - Fleck D
AUID- ORCID: 0000-0002-6692-2388
AD  - Department of Chemosensation, Institute for Biology II, RWTH Aachen University, 
      Aachen D-52074, Germany.
FAU - Rock, Jason R
AU  - Rock JR
AD  - Center for Regenerative Medicine, Boston University School of Medicine, Boston 
      02118, MA.
FAU - Spehr, Marc
AU  - Spehr M
AUID- ORCID: 0000-0001-6616-4196
AD  - Department of Chemosensation, Institute for Biology II, RWTH Aachen University, 
      Aachen D-52074, Germany menini@sissa.it m.spehr@sensorik.rwth-aachen.de.
FAU - Menini, Anna
AU  - Menini A
AUID- ORCID: 0000-0001-9304-0412
AD  - Neurobiology Group, SISSA, International School for Advanced Studies, Trieste 
      34136, Italy menini@sissa.it m.spehr@sensorik.rwth-aachen.de.
FAU - Pifferi, Simone
AU  - Pifferi S
AUID- ORCID: 0000-0002-3687-3984
AD  - Neurobiology Group, SISSA, International School for Advanced Studies, Trieste 
      34136, Italy.
AD  - Department of Experimental and Clinical Medicine, Universita Politecnica delle 
      Marche, Ancona 60126, Italy.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210915
PL  - United States
TA  - eNeuro
JT  - eNeuro
JID - 101647362
RN  - 0 (Pheromones)
SB  - IM
MH  - Action Potentials
MH  - Animals
MH  - Mice
MH  - Mice, Knockout
MH  - *Pheromones
MH  - Sensory Receptor Cells
MH  - *Vomeronasal Organ
PMC - PMC8445037
OTO - NOTNLM
OT  - TMEM16
OT  - ion channel
OT  - sensory
OT  - vomeronasal
EDAT- 2021/08/27 06:00
MHDA- 2021/10/21 06:00
PMCR- 2021/09/15
CRDT- 2021/08/26 05:35
PHST- 2021/04/22 00:00 [received]
PHST- 2021/06/21 00:00 [revised]
PHST- 2021/07/06 00:00 [accepted]
PHST- 2021/08/27 06:00 [pubmed]
PHST- 2021/10/21 06:00 [medline]
PHST- 2021/08/26 05:35 [entrez]
PHST- 2021/09/15 00:00 [pmc-release]
AID - ENEURO.0179-21.2021 [pii]
AID - eN-NWR-0179-21 [pii]
AID - 10.1523/ENEURO.0179-21.2021 [doi]
PST - epublish
SO  - eNeuro. 2021 Sep 15;8(5):ENEURO.0179-21.2021. doi: 10.1523/ENEURO.0179-21.2021. 
      Print 2021 Sep-Oct.