PMID- 34435402
OWN - NLM
STAT- MEDLINE
DCOM- 20211012
LR  - 20211012
IS  - 1365-2184 (Electronic)
IS  - 0960-7722 (Print)
IS  - 0960-7722 (Linking)
VI  - 54
IP  - 10
DP  - 2021 Oct
TI  - Bone marrow-derived mesenchymal stem cells promote Helicobacter pylori-associated 
      gastric cancer progression by secreting thrombospondin-2.
PG  - e13114
LID - 10.1111/cpr.13114 [doi]
LID - e13114
AB  - OBJECTIVES: Bone marrow-derived cells (BMDCs), especially mesenchymal stem cells 
      (MSCs), may be involved in the development of Helicobacter pylori-associated 
      gastric cancer (GC) in mice, but the specific mechanism remains unclear, and 
      evidence from human studies is lacking. MATERIALS AND METHODS: To verify the role 
      of BM-MSCs in H pylori-associated GC, green fluorescent protein (GFP)-labelled 
      BM-MSCs were transplanted into the subserosal layers of the stomach in a mouse 
      model of chronic H pylori infection. Three months post-transplantation, the mice 
      were sacrificed, and the gastric tissues were subjected to histopathological and 
      immunofluorescence analyses. In addition, we performed fluorescence in situ 
      hybridization (FISH) and immunofluorescence analyses of gastric tissue from a 
      female patient with H pylori infection and a history of acute myeloid leukaemia 
      who received a BM transplant from a male donor. RESULTS: In mice with chronic H 
      pylori infection, GFP-labelled BM-MSCs migrated from the serous layer to the 
      mucosal layer and promoted GC progression. The BM-MSCs differentiated into 
      pan-cytokeratin-positive epithelial cells and alpha-smooth muscle actin-positive 
      cancer-associated fibroblasts (CAFs) by secreting the protein thrombospondin-2. 
      FISH analysis of gastric tissue from the female patient revealed 
      Y-chromosome-positive cells. Immunofluorescence analyses further confirmed that 
      Y-chromosome-positive cells showed positive BM-MSCs marker. These results 
      suggested that allogeneic BMDCs, including BM-MSCs, can migrate to the stomach 
      under chronic H pylori infection. CONCLUSIONS: Taken together, these findings 
      imply that BM-MSCs participate in the development of chronic H pylori-associated 
      GC by differentiating into both gastric epithelial cells and CAFs.
CI  - (c) 2021 The Authors. Cell Proliferation published by John Wiley & Sons Ltd.
FAU - Shi, Huiying
AU  - Shi H
AUID- ORCID: 0000-0002-0796-9944
AD  - Department of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, China.
FAU - Qi, Cuihua
AU  - Qi C
AD  - Department of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, China.
FAU - Meng, Lingjun
AU  - Meng L
AD  - Department of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, China.
FAU - Yao, Hailing
AU  - Yao H
AD  - Department of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, China.
FAU - Jiang, Chen
AU  - Jiang C
AD  - Department of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, China.
FAU - Fan, Mengke
AU  - Fan M
AD  - Department of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, China.
FAU - Zhang, Qin
AU  - Zhang Q
AD  - Department of Pathology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, China.
FAU - Hou, Xiaohua
AU  - Hou X
AD  - Department of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, China.
FAU - Lin, Rong
AU  - Lin R
AD  - Department of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, China.
LA  - eng
GR  - 2017YFC0110003/National key research and development program of China/
GR  - 81272656/National Natural Science Foundation of China/
GR  - 81572428/National Natural Science Foundation of China/
GR  - 81974068/National Natural Science Foundation of China/
GR  - 2017CFA061/Natural Science Foundation of Hubei Province/
PT  - Journal Article
DEP - 20210825
PL  - England
TA  - Cell Prolif
JT  - Cell proliferation
JID - 9105195
RN  - 0 (Thrombospondins)
SB  - IM
MH  - Animals
MH  - Bone Marrow/*metabolism/microbiology
MH  - Bone Marrow Cells/*metabolism
MH  - Cell Differentiation/physiology
MH  - Cell Proliferation/physiology
MH  - Epithelial Cells/metabolism/microbiology
MH  - Female
MH  - Gastric Mucosa/*metabolism/microbiology
MH  - Helicobacter Infections/*metabolism/microbiology
MH  - Helicobacter pylori/pathogenicity
MH  - Humans
MH  - Male
MH  - Mesenchymal Stem Cells/*metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Stomach Neoplasms/*metabolism/microbiology
MH  - Thrombospondins/*metabolism
PMC - PMC8488559
OTO - NOTNLM
OT  - Helicobacter pylori
OT  - Bone marrow-derived mesenchymal stem cells (BM-MSCs)
OT  - Cancer-associated fibroblasts (CAFs)
OT  - Gastric cancer (GC)
OT  - Thrombospondin-2
COIS- None.
EDAT- 2021/08/27 06:00
MHDA- 2021/10/13 06:00
PMCR- 2021/08/25
CRDT- 2021/08/26 06:31
PHST- 2021/07/26 00:00 [revised]
PHST- 2021/06/01 00:00 [received]
PHST- 2021/08/02 00:00 [accepted]
PHST- 2021/08/27 06:00 [pubmed]
PHST- 2021/10/13 06:00 [medline]
PHST- 2021/08/26 06:31 [entrez]
PHST- 2021/08/25 00:00 [pmc-release]
AID - CPR13114 [pii]
AID - 10.1111/cpr.13114 [doi]
PST - ppublish
SO  - Cell Prolif. 2021 Oct;54(10):e13114. doi: 10.1111/cpr.13114. Epub 2021 Aug 25.