PMID- 34437823
OWN - NLM
STAT- MEDLINE
DCOM- 20220323
LR  - 20220827
IS  - 1551-4005 (Electronic)
IS  - 1538-4101 (Print)
IS  - 1551-4005 (Linking)
VI  - 20
IP  - 18
DP  - 2021 Sep
TI  - Hsa_circ_0006232 promotes laryngeal squamous cell cancer progression through 
      FUS-mediated EZH2 stabilization.
PG  - 1799-1811
LID - 10.1080/15384101.2021.1959973 [doi]
AB  - Laryngeal squamous cell cancer (LSCC) is one of the most common malignant tumors 
      in head and neck tumors. Our previous study has revealed that hsa_circ_0006232 is 
      abnormally expressed in LSCC. This study attempts to verify the biological role 
      of hsa_circ_0006232 in LSCC. We found that compared with human bronchial 
      epithelial cells, hsa_circ_0006232 was highly expressed in human LSCC cells 
      (AMC-HN-8 and TU686). Moreover, hsa_circ_0006232 promoted proliferation, 
      migration and invasion of AMC-HN-8 and TU686 cells. Hsa_circ_0006232 promoted the 
      expression of enhancer of zeste homolog 2 (EZH2) and repressed the expression of 
      phosphatase and tensin homolog deleted on chromosome 10 (PTEN). Fused in sarcoma 
      (FUS) interacted with hsa_circ_0006232 and EZH2, and FUS promoted the 
      stabilization of EZH2. Hsa_circ_0006232 inhibited PTEN by promoting FUS 
      expression. Moreover, we constructed a tumor xenograft model by injection of 
      AMC-HN-8 cells with hsa_circ_0006232 knockdown, and we found that 
      hsa_circ_0006232 deficiency decreased tumor growth in mice. Hsa_circ_0006232 
      silencing repressed EZH2 expression and enhanced PTEN expression in tumor 
      tissues. In conclusion, our data have demonstrated that Hsa_circ_0006232 promotes 
      proliferation, migration and invasion of LSCC cells, and accelerates tumor growth 
      of LSCC through FUS-mediated EZH2 stabilization. Thus, hsa_circ_0006232 may be a 
      novel therapeutic target in LSCC treatment.
FAU - Wu, Tianyi
AU  - Wu T
AD  - Department of Otolaryngology, Head and Neck Surgery, Henan Provincial People's 
      Hospital; People's Hospital of Zhengzhou University; People's Hospital of Henan 
      University, Zhengzhou, Henan, China.
FAU - Wang, Guangke
AU  - Wang G
AD  - Department of Otolaryngology, Head and Neck Surgery, Henan Provincial People's 
      Hospital; People's Hospital of Zhengzhou University; People's Hospital of Henan 
      University, Zhengzhou, Henan, China.
FAU - Zeng, Xianting
AU  - Zeng X
AD  - Department of Otolaryngology, Head and Neck Surgery, People's Hospital of Henan 
      University, 7 Weiwu Road, Zhengzhou, Henan, China.
FAU - Sun, Zhanwei
AU  - Sun Z
AD  - Department of Otolaryngology, Head and Neck Surgery, Henan Provincial People's 
      Hospital; People's Hospital of Zhengzhou University; People's Hospital of Henan 
      University, Zhengzhou, Henan, China.
FAU - Li, Shichao
AU  - Li S
AD  - Department of Otolaryngology, Head and Neck Surgery, Henan Provincial People's 
      Hospital; People's Hospital of Zhengzhou University; People's Hospital of Henan 
      University, Zhengzhou, Henan, China.
FAU - Wang, Weiwei
AU  - Wang W
AD  - Department of Otolaryngology, Head and Neck Surgery, Henan Provincial People's 
      Hospital; People's Hospital of Zhengzhou University; People's Hospital of Henan 
      University, Zhengzhou, Henan, China.
FAU - Yu, Boyu
AU  - Yu B
AD  - Department of Otolaryngology, Head and Neck Surgery, Henan Provincial People's 
      Hospital; People's Hospital of Zhengzhou University; People's Hospital of Henan 
      University, Zhengzhou, Henan, China.
LA  - eng
PT  - Journal Article
DEP - 20210826
PL  - United States
TA  - Cell Cycle
JT  - Cell cycle (Georgetown, Tex.)
JID - 101137841
RN  - 0 (FUS protein, human)
RN  - 0 (RNA, Circular)
RN  - 0 (RNA-Binding Protein FUS)
RN  - EC 2.1.1.43 (EZH2 protein, human)
RN  - EC 2.1.1.43 (Enhancer of Zeste Homolog 2 Protein)
RN  - EC 3.1.3.67 (PTEN Phosphohydrolase)
RN  - EC 3.1.3.67 (PTEN protein, human)
SB  - IM
MH  - Animals
MH  - Bronchi/cytology
MH  - Carcinogenesis/genetics/*metabolism
MH  - Cell Line, Tumor
MH  - Cell Movement/genetics
MH  - Cell Proliferation/genetics
MH  - *Disease Progression
MH  - Enhancer of Zeste Homolog 2 Protein/genetics/*metabolism
MH  - Epithelial Cells/metabolism
MH  - Gene Silencing
MH  - Humans
MH  - Laryngeal Neoplasms/genetics/*metabolism/pathology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Nude
MH  - PTEN Phosphohydrolase/genetics/metabolism
MH  - RNA, Circular/genetics/*metabolism
MH  - RNA-Binding Protein FUS/genetics/*metabolism
MH  - Signal Transduction/*genetics
MH  - Squamous Cell Carcinoma of Head and Neck/genetics/*metabolism/pathology
MH  - Transfection
MH  - Tumor Burden/genetics
MH  - Xenograft Model Antitumor Assays
PMC - PMC8525971
OTO - NOTNLM
OT  - Hsa_circ_0006232
OT  - invasion
OT  - laryngeal squamous cell cancer
OT  - migration
OT  - proliferation
COIS- All authors declare no competing interest.
EDAT- 2021/08/27 06:00
MHDA- 2022/03/24 06:00
PMCR- 2022/08/26
CRDT- 2021/08/26 20:11
PHST- 2021/08/27 06:00 [pubmed]
PHST- 2022/03/24 06:00 [medline]
PHST- 2021/08/26 20:11 [entrez]
PHST- 2022/08/26 00:00 [pmc-release]
AID - 1959973 [pii]
AID - 10.1080/15384101.2021.1959973 [doi]
PST - ppublish
SO  - Cell Cycle. 2021 Sep;20(18):1799-1811. doi: 10.1080/15384101.2021.1959973. Epub 
      2021 Aug 26.