PMID- 34438779
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210830
IS  - 2076-2615 (Print)
IS  - 2076-2615 (Electronic)
IS  - 2076-2615 (Linking)
VI  - 11
IP  - 8
DP  - 2021 Aug 6
TI  - Steroidogenic Enzyme and Steroid Receptor Expression in the Equine Accessory Sex 
      Glands.
LID - 10.3390/ani11082322 [doi]
LID - 2322
AB  - The expression pattern and distribution of sex steroid receptors and 
      steroidogenic enzymes during development of the equine accessory sex glands has 
      not previously been described. We hypothesized that equine steroidogenic enzyme 
      and sex steroid receptor expression is dependent on reproductive status. 
      Accessory sex glands were harvested from mature stallions, pre-pubertal colts, 
      geldings, and fetuses. Expression of mRNA for estrogen receptor 1 (ESR1), 
      estrogen receptor 2 (ESR2), androgen receptor (AR), 3beta-Hydroxysteroid 
      dehydrogenase/Delta5-4 isomerase (3betaHSD), P450,17alpha hydroxylase, 17-20 lyase (CYP17), 
      and aromatase (CYP19) were quantified by RT-PCR, and protein localization of AR, 
      ER-alpha, ER-beta, and 3betaHSD were investigated by immunohistochemistry. Expression of 
      AR, ESR2, CYP17, or CYP19 in the ampulla was not different across reproductive 
      statuses (p > 0.1), while expression of ESR1 was higher in the ampulla of 
      geldings and fetuses than those of stallions or colts (p < 0.05). AR, ESR1 and 
      ESR2 expression were decreased in stallion vesicular glands compared to the fetus 
      or gelding, while AR, ESR1, and CYP17 expression were decreased in the 
      bulbourethral glands compared to other glands. ESR1 expression was increased in 
      the prostate compared to the bulbourethral glands, and no differences were seen 
      with CYP19 or 3beta-HSD. In conclusion, sex steroid receptors are expressed in all 
      equine male accessory sex glands in all stages of life, while the steroidogenic 
      enzymes were weakly and variably expressed.
FAU - Ellerbrock, Robyn E
AU  - Ellerbrock RE
AUID- ORCID: 0000-0001-7337-8002
AD  - Department of Veterinary Clinical Medicine, College of Veterinary Medicine, 
      University of Illinois Urbana-Champaign, 1008 W Hazelwood Drive, Urbana, IL 
      61802, USA.
FAU - Podico, Giorgia
AU  - Podico G
AUID- ORCID: 0000-0001-8046-6254
AD  - Department of Veterinary Clinical Medicine, College of Veterinary Medicine, 
      University of Illinois Urbana-Champaign, 1008 W Hazelwood Drive, Urbana, IL 
      61802, USA.
FAU - Scoggin, Kirsten E
AU  - Scoggin KE
AUID- ORCID: 0000-0003-1348-641X
AD  - Maxwell H. Gluck Equine Research Center, University of Kentucky, 1400 
      Nicholasville Rd, Lexington, KY 40546, USA.
FAU - Ball, Barry A
AU  - Ball BA
AUID- ORCID: 0000-0002-0502-0276
AD  - Maxwell H. Gluck Equine Research Center, University of Kentucky, 1400 
      Nicholasville Rd, Lexington, KY 40546, USA.
FAU - Carossino, Mariano
AU  - Carossino M
AD  - Maxwell H. Gluck Equine Research Center, University of Kentucky, 1400 
      Nicholasville Rd, Lexington, KY 40546, USA.
FAU - Canisso, Igor F
AU  - Canisso IF
AUID- ORCID: 0000-0003-3799-6641
AD  - Department of Veterinary Clinical Medicine, College of Veterinary Medicine, 
      University of Illinois Urbana-Champaign, 1008 W Hazelwood Drive, Urbana, IL 
      61802, USA.
LA  - eng
PT  - Journal Article
DEP - 20210806
PL  - Switzerland
TA  - Animals (Basel)
JT  - Animals : an open access journal from MDPI
JID - 101635614
PMC - PMC8388441
OTO - NOTNLM
OT  - ampulla
OT  - bulbourethral gland
OT  - fetus
OT  - prostate
OT  - stallion
OT  - vesicular gland
COIS- The authors declare no conflict of interest.
EDAT- 2021/08/28 06:00
MHDA- 2021/08/28 06:01
PMCR- 2021/08/06
CRDT- 2021/08/27 01:01
PHST- 2021/06/19 00:00 [received]
PHST- 2021/07/31 00:00 [revised]
PHST- 2021/08/05 00:00 [accepted]
PHST- 2021/08/27 01:01 [entrez]
PHST- 2021/08/28 06:00 [pubmed]
PHST- 2021/08/28 06:01 [medline]
PHST- 2021/08/06 00:00 [pmc-release]
AID - ani11082322 [pii]
AID - animals-11-02322 [pii]
AID - 10.3390/ani11082322 [doi]
PST - epublish
SO  - Animals (Basel). 2021 Aug 6;11(8):2322. doi: 10.3390/ani11082322.