PMID- 34438923 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20210830 IS - 2076-2615 (Print) IS - 2076-2615 (Electronic) IS - 2076-2615 (Linking) VI - 11 IP - 8 DP - 2021 Aug 23 TI - Altered Proteomic Profile of Adipose Tissue-Derived Mesenchymal Stem Cell Exosomes from Cats with Severe Chronic Gingivostomatitis. LID - 10.3390/ani11082466 [doi] LID - 2466 AB - Feline chronic gingivostomatitis (FCGS) is a pathology with a complicated therapeutic approach and with a prevalence between 0.7 and 12%. Although the etiology of the disease is diverse, feline calicivirus infection is known to be a predisposing factor. To date, the available treatment helps in controlling the disease, but cannot always provide a cure, which leads to a high percentage of refractory animals. Mesenchymal stem cells (MSCs) play a pivotal role in the homeostasis and reparation of different tissues and have the ability to modulate the immune system responses. This ability is, in part, due to the capacity of exosomes to play a part in intercellular cell communication. However, the precise role of MSC-derived exosomes and their alterations in immunocompromised pathologies remains unknown, especially in veterinary patients. The goal of this work was to analyze the proteomic profile of feline adipose tissue-derived MSCs (fAd-MSCs) from calicivirus-positive FCGS patients, and to detect possible modifications of the exosomal cargo, to gain better knowledge of the disease's etiopathogenesis. Using high-resolution mass spectrometry and functional enrichment analysis with Gene Ontology, exosomes isolated from the fAd-MSCs of five healthy cats and five calicivirus-positive FCGS patients, were pooled and compared. The results showed that the fAd-MSCs from cats suffering from FCGS not only had a higher exosome production, but also their exosomes showed significant alterations in their proteomic profile. Eight proteins were exclusively found in the exosomes from the FCGS group, and five proteins could only be found in the exosomes from the healthy cats. When comparing the exosomal cargo between the two groups, significant upregulation of 17 and downregulation of 13 proteins were detected in the FCGS group compared to the control group. These findings shed light on new perspectives on the roles of MSCs and their relation to this disease, which may help in identifying new therapeutic targets and selecting specific biomarkers. FAU - Villatoro, Antonio J AU - Villatoro AJ AD - Laboratory of Bioengineering and Tissue Regeneration, Department of Cell Biology, Genetics and Physiology, University of Malaga, IBIMA, 29071 Malaga, Spain. AD - Instituto de Inmunologia Clinica y Terapia Celular (IMMUNESTEM), 29071 Malaga, Spain. FAU - Martin-Astorga, Maria Del Carmen AU - Martin-Astorga MDC AUID- ORCID: 0000-0003-4784-9944 AD - Laboratory of Bioengineering and Tissue Regeneration, Department of Cell Biology, Genetics and Physiology, University of Malaga, IBIMA, 29071 Malaga, Spain. FAU - Alcoholado, Cristina AU - Alcoholado C AD - Laboratory of Bioengineering and Tissue Regeneration, Department of Cell Biology, Genetics and Physiology, University of Malaga, IBIMA, 29071 Malaga, Spain. FAU - Cardenas, Casimiro AU - Cardenas C AUID- ORCID: 0000-0001-6259-8296 AD - Research Support Central Services (SCAI) of the University of Malaga, 29071 Malaga, Spain. FAU - Farinas, Fernando AU - Farinas F AD - Grupo Ynmun, Spanish Association for the Research in Immunological and Infectious Diseases, 29071 Malaga, Spain. FAU - Becerra, Jose AU - Becerra J AUID- ORCID: 0000-0002-4849-8931 AD - Laboratory of Bioengineering and Tissue Regeneration, Department of Cell Biology, Genetics and Physiology, University of Malaga, IBIMA, 29071 Malaga, Spain. AD - Biomedical Research Networking Center in Bioengineering, Biomaterials, and Nanomedicine (CIBER-BBN), 28029 Madrid, Spain. AD - Andalusian Centre for Nanomedicine and Biotechnology-University of Malaga (BIONAND), 29590 Malaga, Spain. FAU - Visser, Rick AU - Visser R AD - Laboratory of Bioengineering and Tissue Regeneration, Department of Cell Biology, Genetics and Physiology, University of Malaga, IBIMA, 29071 Malaga, Spain. AD - Biomedical Research Networking Center in Bioengineering, Biomaterials, and Nanomedicine (CIBER-BBN), 28029 Madrid, Spain. AD - Andalusian Centre for Nanomedicine and Biotechnology-University of Malaga (BIONAND), 29590 Malaga, Spain. LA - eng GR - RD16/0011/0022/Instituto de Salud Carlos III/ GR - UMA18-FEDERJA-133/Consejeria de Economia, Innovacion, Ciencia y Empleo, Junta de Andalucia/ GR - VI National R&D&I Plan 2008-2011/Centro de Investigacion Biomedica en Red en Bioingenieria, Biomateriales y Nanomedicina/ GR - PEJ2018-004785-A/Ministerio de Ciencia, Innovacion y Universidades/ PT - Journal Article DEP - 20210823 PL - Switzerland TA - Animals (Basel) JT - Animals : an open access journal from MDPI JID - 101635614 PMC - PMC8388770 OTO - NOTNLM OT - exosomes OT - feline OT - feline chronic gingivostomatitis OT - mesenchymal stem cells OT - ultra-high-performance liquid chromatography high-resolution mass spectrometry (UHPLC-HRMS) COIS- The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results. EDAT- 2021/08/28 06:00 MHDA- 2021/08/28 06:01 PMCR- 2021/08/23 CRDT- 2021/08/27 01:02 PHST- 2021/07/22 00:00 [received] PHST- 2021/08/16 00:00 [revised] PHST- 2021/08/19 00:00 [accepted] PHST- 2021/08/27 01:02 [entrez] PHST- 2021/08/28 06:00 [pubmed] PHST- 2021/08/28 06:01 [medline] PHST- 2021/08/23 00:00 [pmc-release] AID - ani11082466 [pii] AID - animals-11-02466 [pii] AID - 10.3390/ani11082466 [doi] PST - epublish SO - Animals (Basel). 2021 Aug 23;11(8):2466. doi: 10.3390/ani11082466.