PMID- 34440227
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240403
IS  - 2227-9059 (Print)
IS  - 2227-9059 (Electronic)
IS  - 2227-9059 (Linking)
VI  - 9
IP  - 8
DP  - 2021 Aug 16
TI  - Plasma-Based Bioinks for Extrusion Bioprinting of Advanced Dressings.
LID - 10.3390/biomedicines9081023 [doi]
LID - 1023
AB  - Extrusion bioprinting based on the development of novel bioinks offers the 
      possibility of manufacturing clinically useful tools for wound management. In 
      this study, we show the rheological properties and printability outcomes of two 
      advanced dressings based on platelet-rich plasma (PRP) and platelet-poor plasma 
      (PPP) blended with alginate and loaded with dermal fibroblasts. Measurements 
      taken at 1 h, 4 days, and 18 days showed that both the PRP- and PPP-based 
      dressings retain plasma and platelet proteins, which led to the upregulation of 
      angiogenic and immunomodulatory proteins by embedded fibroblasts (e.g., an up to 
      69-fold increase in vascular endothelial growth factor (VEGF), an up to 188-fold 
      increase in monocyte chemotactic protein 1 (MCP-1), and an up to 456-fold 
      increase in hepatocyte growth factor (HGF) 18 days after printing). Conditioned 
      media harvested from both PRP and PPP constructs stimulated the proliferation of 
      human umbilical vein endothelial cells (HUVECs), whereas only those from PRP 
      dressings stimulated HUVEC migration, which correlated with the VEGF/MCP-1 and 
      VEGF/HGF ratios. Similarly, the advanced dressings increased the level of 
      interleukin-8 and led to a four-fold change in the level of extracellular matrix 
      protein 1. These findings suggest that careful selection of plasma formulations 
      to fabricate wound dressings can enable regulation of the molecular composition 
      of the microenvironment, as well as paracrine interactions, thereby improving the 
      clinical potential of dressings and providing the possibility to tailor each 
      composition to specific wound types and healing stages.
FAU - Del Amo, Cristina
AU  - Del Amo C
AUID- ORCID: 0000-0002-3796-4482
AD  - Regenerative Therapies, Bioprinting Laboratory, Biocruces Bizkaia Health Research 
      Institute, Cruces University Hospital, 48903 Barakaldo, Spain.
FAU - Perez-Valle, Arantza
AU  - Perez-Valle A
AUID- ORCID: 0000-0002-3108-4978
AD  - Regenerative Therapies, Bioprinting Laboratory, Biocruces Bizkaia Health Research 
      Institute, Cruces University Hospital, 48903 Barakaldo, Spain.
FAU - Perez-Garrastachu, Miguel
AU  - Perez-Garrastachu M
AD  - Department of Cell Biology and Histology, School of Medicine and Nursing, 
      University of the Basque Country, UPV/EHU, 48940 Leioa, Spain.
FAU - Jauregui, Ines
AU  - Jauregui I
AD  - Regenerative Therapies, Bioprinting Laboratory, Biocruces Bizkaia Health Research 
      Institute, Cruces University Hospital, 48903 Barakaldo, Spain.
FAU - Andollo, Noelia
AU  - Andollo N
AUID- ORCID: 0000-0002-8918-6395
AD  - Department of Cell Biology and Histology, School of Medicine and Nursing, 
      University of the Basque Country, UPV/EHU, 48940 Leioa, Spain.
AD  - BEGIKER, BioCruces Bizkaia Health Research Institute, 48903 Barakaldo, Spain.
FAU - Arluzea, Jon
AU  - Arluzea J
AD  - Department of Cell Biology and Histology, School of Medicine and Nursing, 
      University of the Basque Country, UPV/EHU, 48940 Leioa, Spain.
FAU - Guerrero, Pedro
AU  - Guerrero P
AUID- ORCID: 0000-0002-9785-7048
AD  - BIOMAT Research Group, Escuela de Ingenieria de Gipuzkoa Donostia-San Sebastian, 
      University of the Basque Country (UPV/EHU), 20018 Donostia-San Sebastian, Spain.
AD  - BCMaterials, Basque Center for Materials, Applications and Nanostructures, 
      UPV/EHU Science Park, 48940 Leioa, Spain.
FAU - de la Caba, Koro
AU  - de la Caba K
AUID- ORCID: 0000-0002-8866-7314
AD  - BIOMAT Research Group, Escuela de Ingenieria de Gipuzkoa Donostia-San Sebastian, 
      University of the Basque Country (UPV/EHU), 20018 Donostia-San Sebastian, Spain.
AD  - BCMaterials, Basque Center for Materials, Applications and Nanostructures, 
      UPV/EHU Science Park, 48940 Leioa, Spain.
FAU - Andia, Isabel
AU  - Andia I
AUID- ORCID: 0000-0003-0607-4767
AD  - Regenerative Therapies, Bioprinting Laboratory, Biocruces Bizkaia Health Research 
      Institute, Cruces University Hospital, 48903 Barakaldo, Spain.
LA  - eng
GR  - B4H KK-2019-0006-BC/Basque Government, Elkartek Program/
PT  - Journal Article
DEP - 20210816
PL  - Switzerland
TA  - Biomedicines
JT  - Biomedicines
JID - 101691304
PMC - PMC8392180
OTO - NOTNLM
OT  - bioprinting
OT  - cytokines
OT  - growth factors
OT  - platelet-rich plasma
OT  - wound healing
COIS- The authors declare no conflict of interest.
EDAT- 2021/08/28 06:00
MHDA- 2021/08/28 06:01
PMCR- 2021/08/16
CRDT- 2021/08/27 01:06
PHST- 2021/07/14 00:00 [received]
PHST- 2021/08/03 00:00 [revised]
PHST- 2021/08/06 00:00 [accepted]
PHST- 2021/08/27 01:06 [entrez]
PHST- 2021/08/28 06:00 [pubmed]
PHST- 2021/08/28 06:01 [medline]
PHST- 2021/08/16 00:00 [pmc-release]
AID - biomedicines9081023 [pii]
AID - biomedicines-09-01023 [pii]
AID - 10.3390/biomedicines9081023 [doi]
PST - epublish
SO  - Biomedicines. 2021 Aug 16;9(8):1023. doi: 10.3390/biomedicines9081023.