PMID- 34440611
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240403
IS  - 2075-1729 (Print)
IS  - 2075-1729 (Electronic)
IS  - 2075-1729 (Linking)
VI  - 11
IP  - 8
DP  - 2021 Aug 23
TI  - Arf GTPases Are Required for the Establishment of the Pre-Assembly Compartment in 
      the Early Phase of Cytomegalovirus Infection.
LID - 10.3390/life11080867 [doi]
LID - 867
AB  - Shortly after entering the cells, cytomegaloviruses (CMVs) initiate massive 
      reorganization of cellular endocytic and secretory pathways, which results in the 
      forming of the cytoplasmic virion assembly compartment (AC). We have previously 
      shown that the formation of AC in murine CMV- (MCMV) infected cells begins in the 
      early phase of infection (at 4-6 hpi) with the pre-AC establishment. Pre-AC 
      comprises membranes derived from the endosomal recycling compartment, early 
      endosomes, and the trans-Golgi network, which is surrounded by fragmented Golgi 
      cisterns. To explore the importance of Arf GTPases in the biogenesis of the 
      pre-AC, we infected Balb 3T3 cells with MCMV and analyzed the expression and 
      intracellular localization of Arf proteins in the early phases (up to 16 hpi) of 
      infection and the development of pre-AC in cells with a knockdown of Arf protein 
      expression by small interfering RNAs (siRNAs). Herein, we show that even in the 
      early phase, MCMVs cause massive reorganization of the Arf system of the host 
      cells and induce the over-recruitment of Arf proteins onto the membranes of 
      pre-AC. Knockdown of Arf1, Arf3, Arf4, or Arf6 impaired the establishment of 
      pre-AC. However, the knockdown of Arf1 and Arf6 also abolished the establishment 
      of infection. Our study demonstrates that Arf GTPases are required for different 
      steps of early cytomegalovirus infection, including the establishment of the 
      pre-AC.
FAU - Pavisic, Valentino
AU  - Pavisic V
AUID- ORCID: 0000-0003-2291-0278
AD  - Department of Physiology and Immunology, Faculty of Medicine, University of 
      Rijeka, 51000 Rijeka, Croatia.
FAU - Mahmutefendic Lucin, Hana
AU  - Mahmutefendic Lucin H
AUID- ORCID: 0000-0001-8462-4686
AD  - Department of Physiology and Immunology, Faculty of Medicine, University of 
      Rijeka, 51000 Rijeka, Croatia.
AD  - Nursing Department, University North, University Center Varazdin, Jurja Krizanica 
      31b, 42000 Varazdin, Croatia.
FAU - Blagojevic Zagorac, Gordana
AU  - Blagojevic Zagorac G
AUID- ORCID: 0000-0003-1249-3802
AD  - Department of Physiology and Immunology, Faculty of Medicine, University of 
      Rijeka, 51000 Rijeka, Croatia.
AD  - Nursing Department, University North, University Center Varazdin, Jurja Krizanica 
      31b, 42000 Varazdin, Croatia.
FAU - Lucin, Pero
AU  - Lucin P
AD  - Department of Physiology and Immunology, Faculty of Medicine, University of 
      Rijeka, 51000 Rijeka, Croatia.
AD  - Nursing Department, University North, University Center Varazdin, Jurja Krizanica 
      31b, 42000 Varazdin, Croatia.
LA  - eng
GR  - IP-2020-02-1323/Hrvatska Zaklada za Znanost/
GR  - IP-2019-4-3582/Hrvatska Zaklada za Znanost/
GR  - uniri-biomed-18-229/University of Rijeka/
GR  - uniri-biomed-18-88/University of Rijeka/
GR  - uniri-biomed-18-180/University of Rijeka/
PT  - Journal Article
DEP - 20210823
PL  - Switzerland
TA  - Life (Basel)
JT  - Life (Basel, Switzerland)
JID - 101580444
PMC - PMC8399710
OTO - NOTNLM
OT  - Arf GTPases
OT  - Rab10
OT  - cytomegaloviruses
OT  - m06
OT  - pre-assembly compartment
COIS- The authors declare no conflict of interest. The funders had no role in the 
      design of the study; in the collection, analyses, or interpretation of data; in 
      the writing of the manuscript, or in the decision to publish the results.
EDAT- 2021/08/28 06:00
MHDA- 2021/08/28 06:01
PMCR- 2021/08/23
CRDT- 2021/08/27 01:07
PHST- 2021/07/27 00:00 [received]
PHST- 2021/08/16 00:00 [revised]
PHST- 2021/08/18 00:00 [accepted]
PHST- 2021/08/27 01:07 [entrez]
PHST- 2021/08/28 06:00 [pubmed]
PHST- 2021/08/28 06:01 [medline]
PHST- 2021/08/23 00:00 [pmc-release]
AID - life11080867 [pii]
AID - life-11-00867 [pii]
AID - 10.3390/life11080867 [doi]
PST - epublish
SO  - Life (Basel). 2021 Aug 23;11(8):867. doi: 10.3390/life11080867.