PMID- 34441337
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240403
IS  - 2075-4418 (Print)
IS  - 2075-4418 (Electronic)
IS  - 2075-4418 (Linking)
VI  - 11
IP  - 8
DP  - 2021 Aug 3
TI  - Effect of Loading Changes on the Intraventricular Pressure Measured by Color 
      M-Mode Echocardiography in Rats.
LID - 10.3390/diagnostics11081403 [doi]
LID - 1403
AB  - Evaluation of diastolic function is a pivotal challenge due to limitations of the 
      conventional echocardiography, especially when the heart rate is rapid as in 
      rats. Currently, by using color M-mode echocardiography (CMME), intraventricular 
      pressure difference (IVPD) and intraventricular pressure gradient (IVPG) in early 
      diastole can be generated and are available as echocardiographic indices. These 
      indices are expected to be useful for the early diagnosis of heart failure (HF), 
      especially diastolic dysfunction. There have not been any studies demonstrating 
      changes in IVPD and IVPG in response to changes in loading conditions in rats. 
      Therefore, the present study aims to evaluate CMME-derived IVPD and IVPG changes 
      in rats under various loading conditions. Twenty rats were included, divided into 
      two groups for two different experiments, and underwent jugular vein 
      catheterization under inhalational anesthetics. Conventional echocardiography, 
      CMME, and 2D speckle tracking echocardiography were measured at the baseline 
      (BL), after intravenous infusion of milrinone (MIL, n = 10), and after the 
      infusion of hydroxyethyl starch (HES, n = 10). Left ventricular IVPD and IVPG 
      were calculated from color M-mode images and categorized into total, basal, 
      mid-to-apical, mid, and apical parts, and the percentage of the corresponding 
      part was calculated. In comparison to the BL, the ejection fraction, 
      mid-to-apical IVPG, mid IVPG, and apical IVPD were significantly increased after 
      MIL administration (p < 0.05); meanwhile, the end-diastolic volume, E-wave 
      velocity, total IVPD, and basal IVPD were significantly increased with the 
      administration of HES (p < 0.05). The increase in mid-to-apical IVPD, mid IVPD, 
      and apical IVPD indicated increased relaxation. A significant increase in basal 
      IVPD reflected volume overloading by HES. CMME-derived IVPD and IVPG are useful 
      tools for the evaluation of various loading conditions in rats. The approach used 
      in this study provides a model for continuous data acquisition in chronic cardiac 
      disease models without drug testing.
FAU - Yairo, Akira
AU  - Yairo A
AD  - Laboratory of Veterinary Surgery, Department of Veterinary Medicine, Tokyo 
      University of Agriculture and Technology, Tokyo 183-0054, Japan.
FAU - Mandour, Ahmed S
AU  - Mandour AS
AUID- ORCID: 0000-0001-6256-4539
AD  - Laboratory of Veterinary Surgery, Department of Veterinary Medicine, Tokyo 
      University of Agriculture and Technology, Tokyo 183-0054, Japan.
AD  - Department of Animal Medicine (Internal Medicine), Faculty of Veterinary 
      Medicine, Suez Canal University, Ismailia 41522, Egypt.
FAU - Matsuura, Katsuhiro
AU  - Matsuura K
AUID- ORCID: 0000-0002-6047-2865
AD  - Laboratory of Veterinary Surgery, Department of Veterinary Medicine, Tokyo 
      University of Agriculture and Technology, Tokyo 183-0054, Japan.
FAU - Yoshida, Tomohiko
AU  - Yoshida T
AD  - Laboratory of Veterinary Surgery, Department of Veterinary Medicine, Tokyo 
      University of Agriculture and Technology, Tokyo 183-0054, Japan.
FAU - Ma, Danfu
AU  - Ma D
AUID- ORCID: 0000-0002-6557-7703
AD  - Laboratory of Veterinary Surgery, Department of Veterinary Medicine, Tokyo 
      University of Agriculture and Technology, Tokyo 183-0054, Japan.
FAU - Kitpipatkun, Pitipat
AU  - Kitpipatkun P
AD  - Laboratory of Veterinary Surgery, Department of Veterinary Medicine, Tokyo 
      University of Agriculture and Technology, Tokyo 183-0054, Japan.
FAU - Kato, Konosuke
AU  - Kato K
AD  - Laboratory of Veterinary Surgery, Department of Veterinary Medicine, Tokyo 
      University of Agriculture and Technology, Tokyo 183-0054, Japan.
FAU - Cheng, Chieh-Jen
AU  - Cheng CJ
AUID- ORCID: 0000-0003-3376-5440
AD  - Laboratory of Veterinary Surgery, Department of Veterinary Medicine, Tokyo 
      University of Agriculture and Technology, Tokyo 183-0054, Japan.
FAU - El-Husseiny, Hussein M
AU  - El-Husseiny HM
AUID- ORCID: 0000-0003-1143-4018
AD  - Laboratory of Veterinary Surgery, Department of Veterinary Medicine, Tokyo 
      University of Agriculture and Technology, Tokyo 183-0054, Japan.
AD  - Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary 
      Medicine, Benha University, Moshtohor, Toukh, Elqaliobiya 13736, Egypt.
FAU - Tanaka, Takashi
AU  - Tanaka T
AUID- ORCID: 0000-0003-2844-2356
AD  - Laboratory of Veterinary Surgery, Department of Veterinary Medicine, Tokyo 
      University of Agriculture and Technology, Tokyo 183-0054, Japan.
FAU - Shimada, Kazumi
AU  - Shimada K
AUID- ORCID: 0000-0002-2514-695X
AD  - Laboratory of Veterinary Surgery, Department of Veterinary Medicine, Tokyo 
      University of Agriculture and Technology, Tokyo 183-0054, Japan.
FAU - Hamabe, Lina
AU  - Hamabe L
AD  - Laboratory of Veterinary Surgery, Department of Veterinary Medicine, Tokyo 
      University of Agriculture and Technology, Tokyo 183-0054, Japan.
FAU - Uemura, Akiko
AU  - Uemura A
AUID- ORCID: 0000-0003-2671-5074
AD  - Department of Veterinary Surgery, Division of Veterinary Research, Obihiro 
      University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Hokkaido, 
      Japan.
FAU - Takahashi, Ken
AU  - Takahashi K
AD  - Department of Pediatrics and Adolescent Medicine, Juntendo University Graduate 
      School of Medicine, Tokyo 113-8421, Japan.
FAU - Tanaka, Ryou
AU  - Tanaka R
AUID- ORCID: 0000-0001-9948-6490
AD  - Laboratory of Veterinary Surgery, Department of Veterinary Medicine, Tokyo 
      University of Agriculture and Technology, Tokyo 183-0054, Japan.
LA  - eng
GR  - JP18K08086/Japan Society for the Promotion of Science/
PT  - Journal Article
DEP - 20210803
PL  - Switzerland
TA  - Diagnostics (Basel)
JT  - Diagnostics (Basel, Switzerland)
JID - 101658402
PMC - PMC8391789
OTO - NOTNLM
OT  - color m-mode echocardiography
OT  - diastolic function
OT  - heart failure
OT  - intraventricular pressure
OT  - preload
COIS- The authors declare no competing interests.
EDAT- 2021/08/28 06:00
MHDA- 2021/08/28 06:01
PMCR- 2021/08/03
CRDT- 2021/08/27 01:09
PHST- 2021/06/21 00:00 [received]
PHST- 2021/07/28 00:00 [revised]
PHST- 2021/07/31 00:00 [accepted]
PHST- 2021/08/27 01:09 [entrez]
PHST- 2021/08/28 06:00 [pubmed]
PHST- 2021/08/28 06:01 [medline]
PHST- 2021/08/03 00:00 [pmc-release]
AID - diagnostics11081403 [pii]
AID - diagnostics-11-01403 [pii]
AID - 10.3390/diagnostics11081403 [doi]
PST - epublish
SO  - Diagnostics (Basel). 2021 Aug 3;11(8):1403. doi: 10.3390/diagnostics11081403.