PMID- 34441979
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210831
IS  - 2077-0383 (Print)
IS  - 2077-0383 (Electronic)
IS  - 2077-0383 (Linking)
VI  - 10
IP  - 16
DP  - 2021 Aug 19
TI  - Enhanced Expression but Decreased Specific Activity of Matrix Metalloproteinase 
      10 (MMP-10) in Comparison with Matrix Metalloproteinase 3 (MMP-3) in Human 
      Urinary Bladder Carcinoma.
LID - 10.3390/jcm10163683 [doi]
LID - 3683
AB  - Human urinary bladder cancer is a huge worldwide oncological problem causing many 
      deaths every year. The degradation of extracellular matrix (ECM) induced by 
      molecules such as matrix metalloproteinases (MMPs) is one of the main factors 
      influencing the process of metastasis origination. The MMP expression is tied to 
      tumor aggressiveness, stage, and patient prognosis. The cleavage of constituent 
      proteins is initiated and prolonged by matrix metalloproteinases, such as MMP-3 
      and MMP-10. The aim of this study was to evaluate the expression and activity of 
      both MMPs in human urinary bladder cancer occurring at various stages of the 
      disease. Tissue samples from patients with urinary bladder cancer were analyzed. 
      Samples were collected from patients with a low- and high-grade cancer. Control 
      tissue was collected from the site opposite to the tumor. DNA content, MMPs 
      content, and activity of MMP-3 and MMP-10 were measured using ELISA and Western 
      blot techniques. MMP-3 and MMP-10 occur in high molecular complexes in human 
      urinary bladder in healthy and cancerous tissues. Particularly, in high-grade 
      tumors, the content of MMP-10 prevails over MMP-3. The actual and specific 
      activities vary in both grades of urinary bladder cancer; however, the highest 
      activity for MMP-3 and MMP-10 was found in low-grade tissues. In conclusion, 
      MMP-10 had a higher content, but a lower activity in all investigated tissues 
      compared to MMP-3. Generally, obtained results demonstrated a contrary 
      participation of MMP-3 and MMP-10 in ECM remodeling what may be crucial in the 
      pathogenesis of human urinary bladder carcinoma.
FAU - Kudelski, Jacek
AU  - Kudelski J
AD  - Department of Urology, Medical University of Bialystok, M. Sklodowskiej-Curie 24A 
      St., 15-276 Bialystok, Poland.
FAU - Mlynarczyk, Grzegorz
AU  - Mlynarczyk G
AD  - Department of Urology, Medical University of Bialystok, M. Sklodowskiej-Curie 24A 
      St., 15-276 Bialystok, Poland.
AD  - Department of Medical Biochemistry, Medical University of Bialystok, Adama 
      Mickiewicza 2C St., 15-089 Bialystok, Poland.
FAU - Gudowska-Sawczuk, Monika
AU  - Gudowska-Sawczuk M
AUID- ORCID: 0000-0003-4854-7155
AD  - Department of Biochemical Diagnostics, Medical University of Bialystok, 
      Waszyngtona 15A St., 15-269 Bialystok, Poland.
FAU - Mroczko, Barbara
AU  - Mroczko B
AUID- ORCID: 0000-0002-4075-8479
AD  - Department of Biochemical Diagnostics, Medical University of Bialystok, 
      Waszyngtona 15A St., 15-269 Bialystok, Poland.
AD  - Department of Neurodegeneration Diagnostics, Medical University of Bialystok, 
      Waszyngtona 15A St., 15-269 Bialystok, Poland.
FAU - Darewicz, Barbara
AU  - Darewicz B
AD  - Department of Urology, Medical University of Bialystok, M. Sklodowskiej-Curie 24A 
      St., 15-276 Bialystok, Poland.
FAU - Bruczko-Goralewska, Marta
AU  - Bruczko-Goralewska M
AD  - Department of Medical Biochemistry, Medical University of Bialystok, Adama 
      Mickiewicza 2C St., 15-089 Bialystok, Poland.
FAU - Sobolewski, Krzysztof
AU  - Sobolewski K
AD  - Department of Medical Biochemistry, Medical University of Bialystok, Adama 
      Mickiewicza 2C St., 15-089 Bialystok, Poland.
FAU - Romanowicz, Lech
AU  - Romanowicz L
AD  - Department of Medical Biochemistry, Medical University of Bialystok, Adama 
      Mickiewicza 2C St., 15-089 Bialystok, Poland.
LA  - eng
PT  - Journal Article
DEP - 20210819
PL  - Switzerland
TA  - J Clin Med
JT  - Journal of clinical medicine
JID - 101606588
PMC - PMC8397099
OTO - NOTNLM
OT  - MMP-10
OT  - MMP-3
OT  - biomarker
OT  - cancer
OT  - urinary bladder carcinoma
COIS- The authors declare no conflict of interest.
EDAT- 2021/08/28 06:00
MHDA- 2021/08/28 06:01
PMCR- 2021/08/19
CRDT- 2021/08/27 01:11
PHST- 2021/06/22 00:00 [received]
PHST- 2021/08/04 00:00 [revised]
PHST- 2021/08/16 00:00 [accepted]
PHST- 2021/08/27 01:11 [entrez]
PHST- 2021/08/28 06:00 [pubmed]
PHST- 2021/08/28 06:01 [medline]
PHST- 2021/08/19 00:00 [pmc-release]
AID - jcm10163683 [pii]
AID - jcm-10-03683 [pii]
AID - 10.3390/jcm10163683 [doi]
PST - epublish
SO  - J Clin Med. 2021 Aug 19;10(16):3683. doi: 10.3390/jcm10163683.