PMID- 34443562
OWN - NLM
STAT- MEDLINE
DCOM- 20210906
LR  - 20210906
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 26
IP  - 16
DP  - 2021 Aug 17
TI  - Determination of Methylene Blue and Its Metabolite Residues in Aquatic Products 
      by High-Performance Liquid Chromatography-Tandem Mass Spectrometry.
LID - 10.3390/molecules26164975 [doi]
LID - 4975
AB  - A sensitive and reliable method was developed to determine methylene blue (MB) 
      and its metabolite residues, including azure A (AZA), azure B (AZB), and azure C 
      (AZC) in aquatic products by HPLC-MS/MS. The samples were extracted by 
      acetonitrile and cleaned up by alumina-neutral (ALN) cartridges. The analytes 
      were separated on a Sunfire C18 column (150 mm x 2.1 mm, 5 microm). The method was 
      validated according to the European criteria of Commission Decision 2002/657/CE. 
      Good linearity between 1-500 microg/L was obtained with correlation coefficients 
      (R(2)) greater than 0.99. The limit of quantification (LOQ) was 1.0 microg/kg. The 
      average recoveries at three levels of each compound (1, 5, and 10 microg/kg) were 
      demonstrated to be in the range of 71.8-97.5%, with relative standard deviations 
      (RSDs) from 1.05% to 8.63%. This method was suitable for the detection of 
      methylene blue and its metabolite residues in aquatic products.
FAU - Zhang, Xuan
AU  - Zhang X
AD  - East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Shanghai 200090, China.
AD  - Key Laboratory of East China Sea Fisheries Research Institute, Chinese Academy of 
      Fishery Sciences, Shanghai 200090, China.
FAU - Hui, Yunhua
AU  - Hui Y
AD  - East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Shanghai 200090, China.
FAU - Fang, Changling
AU  - Fang C
AD  - East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Shanghai 200090, China.
AD  - Key Laboratory of East China Sea Fisheries Research Institute, Chinese Academy of 
      Fishery Sciences, Shanghai 200090, China.
FAU - Wang, Yuan
AU  - Wang Y
AD  - East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Shanghai 200090, China.
FAU - Han, Feng
AU  - Han F
AD  - East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Shanghai 200090, China.
AD  - Key Laboratory of East China Sea Fisheries Research Institute, Chinese Academy of 
      Fishery Sciences, Shanghai 200090, China.
FAU - Lou, Xiaoyi
AU  - Lou X
AD  - East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Shanghai 200090, China.
AD  - Key Laboratory of East China Sea Fisheries Research Institute, Chinese Academy of 
      Fishery Sciences, Shanghai 200090, China.
FAU - Fodjo, Essy Kouadio
AU  - Fodjo EK
AUID- ORCID: 0000-0002-7915-7216
AD  - Physical Chemistry Laboratory, UFR SSMT, Universite Felix Houphouet Boigny, 
      Abidjan 22 BP 582, Cote d'Ivoire.
FAU - Cai, Youqiong
AU  - Cai Y
AD  - East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Shanghai 200090, China.
FAU - Kong, Cong
AU  - Kong C
AUID- ORCID: 0000-0001-8084-2133
AD  - East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Shanghai 200090, China.
AD  - Key Laboratory of East China Sea Fisheries Research Institute, Chinese Academy of 
      Fishery Sciences, Shanghai 200090, China.
LA  - eng
GR  - 19YF1459900/the Shanghai Sailing Program/
GR  - 2019T13/Central Public-interest Scientific Institution Basal Research Fund for 
      Chinese Academy of Tropical Agricultural Sciences/
PT  - Journal Article
DEP - 20210817
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - T42P99266K (Methylene Blue)
SB  - IM
MH  - *Chromatography, High Pressure Liquid
MH  - Drug Residues/analysis/metabolism
MH  - Limit of Detection
MH  - Linear Models
MH  - Methylene Blue/*analysis/metabolism
MH  - *Tandem Mass Spectrometry
PMC - PMC8401997
OTO - NOTNLM
OT  - aquatic products
OT  - disinfectant
OT  - high-performance liquid chromatography-tandem mass spectrometry
OT  - methylene blue
COIS- The authors declare no conflict of interest.
EDAT- 2021/08/28 06:00
MHDA- 2021/09/07 06:00
PMCR- 2021/08/17
CRDT- 2021/08/27 01:16
PHST- 2021/07/24 00:00 [received]
PHST- 2021/08/10 00:00 [revised]
PHST- 2021/08/13 00:00 [accepted]
PHST- 2021/08/27 01:16 [entrez]
PHST- 2021/08/28 06:00 [pubmed]
PHST- 2021/09/07 06:00 [medline]
PHST- 2021/08/17 00:00 [pmc-release]
AID - molecules26164975 [pii]
AID - molecules-26-04975 [pii]
AID - 10.3390/molecules26164975 [doi]
PST - epublish
SO  - Molecules. 2021 Aug 17;26(16):4975. doi: 10.3390/molecules26164975.